2012
Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
Curwin AJ, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Molecular Biology Of The Cell 2012, 23: 2327-2338. PMID: 22553351, PMCID: PMC3374751, DOI: 10.1091/mbc.e11-09-0826.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsBlotting, WesternCathepsin ACell MembraneCofilin 1EndocytosisGlucan Endo-1,3-beta-D-GlucosidaseGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, FluorescenceMutationProtein TransportProton-Translocating ATPasesR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-Golgi NetworkVacuolesConceptsTrans-Golgi networkLate Golgi membranesCarboxypeptidase YSecretory cargoGolgi membranesLate Golgi compartmentMechanisms of cargoSoluble secretory proteinsRole of cofilinATPase Pma1Yeast orthologueSynthetic sicknessProtein cofilinSpecific cargoMutant cellsMammalian cellsGolgi compartmentSorting defectsPlasma membraneSecretory proteinsGolgi apparatusATPase 1CofilinCell surfacePMR1
2009
Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2009, 187: 1055-1069. PMID: 20026655, PMCID: PMC2806282, DOI: 10.1083/jcb.200908040.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkADF/cofilinActin-depolymerizing factorSecretory proteinsSecretory cargoCofilin knockdownMass spectrometry-based protein profilingMammalian tissue culture cellsGolgi-resident proteinsSorting of proteinsEndogenous secretory proteinsIntegral membrane proteinsSoluble secretory proteinsStable isotope labelingTissue culture cellsDrosophila melanogasterActin remodelingMammalian cellsMembrane proteinsGolgi membranesProtein profilingIsotope labelingCofilinCell surfaceAmino acids