2021
Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells.
Pakdel M, Pacheco-Fernandez N, von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells. Bio-protocol 2021, 11: e3958. PMID: 33855118, PMCID: PMC8032492, DOI: 10.21769/bioprotoc.3958.Peer-Reviewed Original ResearchProtein traffickingVesicle traffickingSecretory pathwayGolgi vesicle traffickingSelective hooks (RUSH) systemHeLa cellsLive-cell microscopyMammalian cellsProtein cargoLive-cell monitoringCargo vesiclesVesicle trackingTraffickingRUSH systemSorting assaysWide-field microscopyPhysiological conditionsVesiclesMore detail processesPathwayCargoCellsFinal destinationProteinSecretory
2018
Exploring new routes for secretory protein export from the trans-Golgi network
Pakdel M, von Blume J. Exploring new routes for secretory protein export from the trans-Golgi network. Molecular Biology Of The Cell 2018, 29: 235-240. PMID: 29382805, PMCID: PMC5996961, DOI: 10.1091/mbc.e17-02-0117.Peer-Reviewed Original ResearchConceptsSecretory cargoSecretory proteinsCytoplasmic actin cytoskeletonMajor sorting stationTrans-Golgi networkGolgi residentsProtein exportGolgi networkActin cytoskeletonATPase SPCA1Cellular compartmentsTissue homeostasisSorting stationSoluble proteinTGNLysosomal hydrolasesOverall pathwayLuminal CaRecent discoveryProteinCargoSpecific carrierSortingCellsFinal destination
2017
Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network
Blank B, von Blume J. Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network. European Journal Of Cell Biology 2017, 96: 383-390. PMID: 28372832, DOI: 10.1016/j.ejcb.2017.03.001.Peer-Reviewed Original ResearchConceptsMolecular functionsCargo moleculesCytoplasmic actin cytoskeletonSpecific cargo moleculesTransmembrane cargo receptorsTrans-Golgi networkMannose-6-phosphate receptorCargo receptorsGolgi networkActin cytoskeletonATPase SPCA1Secreted proteinsPlasma membraneSecretory proteinsExtracellular proteasesCab45Sorting mechanismSurface receptorsProteinExtracellular spaceSpecific carrierPossible roleFinal destinationSortingReceptors