2013
Transmitting the allosteric signal in methylglyoxal synthase
Falahati H, Pazhang M, Zareian S, Ghaemi N, Rofougaran R, Hofer A, Rezaie AR, Khajeh K. Transmitting the allosteric signal in methylglyoxal synthase. Protein Engineering Design And Selection 2013, 26: 445-452. PMID: 23592737, DOI: 10.1093/protein/gzt014.Peer-Reviewed Original ResearchConceptsAllosteric signalMethylglyoxal synthaseEnzyme methylglyoxal synthaseSite of phosphateActive site amino acidsSite-directed mutagenesisSite amino acidsAllosteric networkDynamic proteinsTerminal tailNew salt bridgesSer-55Gly-56Allosteric mechanismActive siteAsp-10Val-101Arg-97AllosteryThermus spAmino acidsPhosphoryl groupHomotropic allosterySalt bridgeModel system
2010
Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5
Pazhang M, Khajeh K, Asghari SM, Falahati H, Naderi-Manesh H. Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5. Applied Biochemistry And Biotechnology 2010, 162: 1519-1528. PMID: 20419481, DOI: 10.1007/s12010-010-8933-0.Peer-Reviewed Original ResearchConceptsMethylglyoxal synthaseThermus spAmino acid sequenceGel filtration experimentsAcid sequenceHexameric enzymeAllosteric inhibitorsQuaternary structureEnzyme subunitMolecular massAmino acidsArg-150GH5Q-SepharoseGenesTMGSSpEnzymeSynthasePresence of phosphateOptimum activityHill coefficientCloningSubunitsPolypeptide