2020
Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis
Uyhazi KE, Yang Y, Liu N, Qi H, Huang XA, Mak W, Weatherbee SD, de Prisco N, Gennarino VA, Song X, Lin H. Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 7851-7862. PMID: 32198202, PMCID: PMC7148564, DOI: 10.1073/pnas.1916471117.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell Self RenewalEmbryonic DevelopmentGene Expression RegulationMammalsMicePluripotent Stem CellsRNA StabilityRNA, MessengerRNA-Binding ProteinsConceptsEmbryonic stem cellsTarget messenger RNAsPumilio proteinsPUM proteinsMessenger RNAEssential functionsStem cell maintenanceDistinct regulatory mechanismsEmbryonic day 8.5ESC pluripotencyTranslational regulatorPluripotency genesGene regulationEarly embryogenesisDifferentiation genesPosttranscriptional levelHigh homologyMRNA stabilityRegulatory mechanismsDouble mutant micePluripotencyDay 8.5Morula stagePluripotency markersEmbryogenesis
2019
Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea
Wang C, Yang ZZ, Guo FH, Shi S, Han XS, Zeng A, Lin H, Jing Q. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. Journal Of Biological Chemistry 2019, 294: 9873-9887. PMID: 31076507, PMCID: PMC6597837, DOI: 10.1074/jbc.ra118.004445.Peer-Reviewed Original ResearchConceptsPIWI proteinsPIWI-interacting RNA (piRNA) biogenesisPlanarian adult stem cellsHeat shock protein 40 family membersDifferent evolutionary lineagesPlanarian Schmidtea mediterraneaStem cell maintenanceStem cell regulationCo-immunoprecipitation assaysStem cellsSomatic stem cellsControl of proteinAdult stem cellsHuman gastric cancer cellsPiRNA biogenesisRNA biogenesisEvolutionary lineagesPiwi-like RNASchmidtea mediterraneaTwo-hybridSMEDWI-1Planarian speciesCell maintenanceGastric cancer cellsPlanarian regeneration
2018
A critical role for nucleoporin 358 (Nup358) in transposon silencing and piRNA biogenesis in Drosophila
Parikh RY, Lin H, Gangaraju VK. A critical role for nucleoporin 358 (Nup358) in transposon silencing and piRNA biogenesis in Drosophila. Journal Of Biological Chemistry 2018, 293: 9140-9147. PMID: 29735528, PMCID: PMC6005430, DOI: 10.1074/jbc.ac118.003264.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsArgonaute ProteinsDNA Transposable ElementsDrosophilaDrosophila ProteinsFemaleGene Expression RegulationGene SilencingGenomic InstabilityGerm CellsMaleMolecular ChaperonesNuclear Pore Complex ProteinsProtein Interaction MapsRNA, Small InterferingTranscription, GeneticConceptsPIWI-interacting RNAsPing-pong cycleNuclear pore complexPiRNA biogenesisGermline knockdownPiRNA pathwayAntisense Piwi-interacting RNAsPiRNA precursor transcriptionSmall noncoding RNAsPiwi functionSilence transposonsPIWI proteinsShort hairpin RNACritical roleArgonaute 3Pore complexNoncoding RNAsGenomic instabilityNuclear localizationGene expressionTransposonNup358Germ cellsBiogenesisHairpin RNA
2014
Retrotransposons and pseudogenes regulate mRNAs and lncRNAs via the piRNA pathway in the germline
Watanabe T, Cheng EC, Zhong M, Lin H. Retrotransposons and pseudogenes regulate mRNAs and lncRNAs via the piRNA pathway in the germline. Genome Research 2014, 25: 368-380. PMID: 25480952, PMCID: PMC4352877, DOI: 10.1101/gr.180802.114.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPiRNA pathwayRetrotransposon sequencesIntergenic regionMammalian PIWI-interacting RNAsRNA regulatory networkLate spermatocytesVivo functional analysisDegradation of mRNAUTR of mRNAsSlicer activityEukaryotic genomesLncRNA transcriptomeRegulatory networksRegulatory sequencesRepetitive sequencesPseudogenesMRNA stabilityFunctional analysisLncRNAsWidespread expressionSpermatid stageRetrotransposonsMRNATransposonPosttranscriptional Regulation of Gene Expression by Piwi Proteins and piRNAs
Watanabe T, Lin H. Posttranscriptional Regulation of Gene Expression by Piwi Proteins and piRNAs. Molecular Cell 2014, 56: 18-27. PMID: 25280102, PMCID: PMC4185416, DOI: 10.1016/j.molcel.2014.09.012.Peer-Reviewed Original ResearchMeSH KeywordsArgonaute ProteinsGene Expression RegulationModels, GeneticRNA Processing, Post-TranscriptionalRNA StabilityRNA, MessengerRNA, Small InterferingSex Determination ProcessesConceptsPIWI-interacting RNAsPIWI proteinsPosttranscriptional regulationPiRNA biogenesisPiRNA pathwayTransposon RNARNA regulationSex determinationCell maintenancePosttranscriptional levelGene expressionDevelopmental transitionsBiological processesProteinRNAGametogenesisRegulationRecent findingsMRNAPotential importancePsuedogenesBiogenesisEmbryogenesisTransposonPathwayNoncoding RNAs in the regulation of DNA replication
Ge XQ, Lin H. Noncoding RNAs in the regulation of DNA replication. Trends In Biochemical Sciences 2014, 39: 341-343. PMID: 25027733, PMCID: PMC4265214, DOI: 10.1016/j.tibs.2014.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA ReplicationGene Expression RegulationHumansRNA, UntranslatedTranscription, Genetic
2013
PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells
Juliano CE, Reich A, Liu N, Götzfried J, Zhong M, Uman S, Reenan RA, Wessel GM, Steele RE, Lin H. PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 111: 337-342. PMID: 24367095, PMCID: PMC3890812, DOI: 10.1073/pnas.1320965111.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsCell DifferentiationCell LineageCell SeparationCytoplasmEpithelial CellsFlow CytometryGene Expression RegulationHydraPhylogenyRecombinant ProteinsRNARNA InterferenceRNA Processing, Post-TranscriptionalRNA, Small InterferingSpecies SpecificityStem CellsTranscriptomeTransgenesConceptsPIWI-interacting RNAsPIWI proteinsStem/progenitor cellsProgenitor cellsPIWI-piRNA pathwayPing-pong signatureSomatic stem/progenitor cellsStem cell functionalitySomatic stem cellsInterstitial lineageNonbilaterian animalsPiwi functionPiRNA biogenesisAnimal germlineTransposon transcriptsSimple metazoanCnidarian HydraSmall RNAsEndodermal lineagesRNA functionPosttranscriptional regulatorsEpithelial lineageLineagesLikely actsPiwi
2011
Small Noncoding RNAs in the Germline
Saxe JP, Lin H. Small Noncoding RNAs in the Germline. Cold Spring Harbor Perspectives In Biology 2011, 3: a002717. PMID: 21669983, PMCID: PMC3181032, DOI: 10.1101/cshperspect.a002717.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsFemaleGene Expression RegulationGerm CellsMaleModels, BiologicalRNA, Small UntranslatedSignal TransductionConceptsRNA pathwaysSmall noncoding RNAsTarget mRNAsNoncoding RNAsSmall RNA pathwaysPiRNA pathwayGermline specificationMicroRNA pathwayMiRNA pathwayGene regulationMRNA translationSomatic tissuesEpigenetic programmingDiverse functionsGene expressionRegulatory functionsGermlinePotent regulatorPathwayRNAMRNARegulationPiwiBiogenesisTransposon
2009
MicroRNAs: key regulators of stem cells
Gangaraju VK, Lin H. MicroRNAs: key regulators of stem cells. Nature Reviews Molecular Cell Biology 2009, 10: 116-125. PMID: 19165214, PMCID: PMC4118578, DOI: 10.1038/nrm2621.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineageGene Expression RegulationHumansMicroRNAsRNA, Small InterferingSequence Analysis, DNASignal TransductionStem CellsTranscription, GeneticConceptsPIWI-interacting RNAsAdult tissue stem cellsStem cell processesTissue stem cellsSmall RNAsStem cellsCell processesNext-generation sequencing technologiesKey protein factorsLong non-coding RNAsNon-coding RNAsNumber of miRNAsGeneration sequencing technologyTranscriptional machineryNew miRNAsAsymmetric divisionDaughter cellsTarget mRNAsProtein factorsMaster regulatorSequencing technologiesKey regulatorUntranslated regionDifferentiation pathwayMiRNAs
2006
MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis
Grivna ST, Pyhtila B, Lin H. MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 13415-13420. PMID: 16938833, PMCID: PMC1569178, DOI: 10.1073/pnas.0605506103.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsGene Expression RegulationMaleMiceMice, Inbred StrainsMicroRNAsMicroscopy, FluorescenceProtein BiosynthesisProteinsRNARNA, MessengerSpermatogenesisTestisConceptsPIWI-interacting RNAsSmall RNAsTranslational machineryMRNA cap-binding complexArgonaute family proteinsCap-binding complexPIWI family membersPresence of DicerRound spermatidsSubset of miRNAsCytosolic ribonucleoproteinTranslational repressionGene regulationFamily proteinsSubfamily proteinsCytoplasmic proteinsTarget mRNAsMIWIPosttranscriptional levelPolysomal fractionsPolysome fractionsMature spermGene expressionImportant regulatorSpermiogenesis
2002
miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis
Deng W, Lin H. miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis. Developmental Cell 2002, 2: 819-830. PMID: 12062093, DOI: 10.1016/s1534-5807(02)00165-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArgonaute ProteinsChromosome MappingCloning, MolecularCyclic AMP Response Element ModulatorCytoplasmDNA-Binding ProteinsGene Expression RegulationInfertility, MaleLIM Domain ProteinsMaleMiceMice, KnockoutMolecular Sequence DataProtein IsoformsProteinsRepressor ProteinsRNA, MessengerSpermatogenesisTestisTranscription FactorsConceptsTarget genesPiwi family genesCytoplasmic protein essentialRound spermatid stageDiverse organismsPiwi genesTranslational regulationFamily genesProtein essentialCytoplasmic proteinsMaster regulatorMurine homologMIWIGenesSpermatid stageStem cellsMRNASpermiogenesisCREMSpermatogenesisSpermatogenic arrestPiwiHomologEncodesCloning