2007
Development and Physiological Regulation of Intestinal Lipid Absorption. II. Dietary lipid absorption, complex lipid synthesis, and the intracellular packaging and secretion of chylomicrons
Mansbach CM, Gorelick F. Development and Physiological Regulation of Intestinal Lipid Absorption. II. Dietary lipid absorption, complex lipid synthesis, and the intracellular packaging and secretion of chylomicrons. AJP Gastrointestinal And Liver Physiology 2007, 293: g645-g650. PMID: 17627968, DOI: 10.1152/ajpgi.00299.2007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChylomicronsDietary FatsEndoplasmic ReticulumHumansIntestinal AbsorptionLipid MetabolismTriglyceridesConceptsLipid absorptionSecretion of chylomicronsEpidemic of obesityDietary lipid absorptionDietary fat absorptionIntestinal lipid absorptionFatty acid transportersFatty acid uptakeDiscovery of familiesLiver fatty acidEndoplasmic reticulumAcid transportersClinical importanceFat absorptionPhysiological functionsIntracellular packagingComplex lipid synthesisLipid synthesisChylomicronsAcid uptakePhysiological regulationRecent advancesAcyltransferasesGolgiObesity
2002
COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle
Siddiqi SA, Gorelick FS, Mahan JT, Mansbach CM. COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle. Journal Of Cell Science 2002, 116: 415-427. PMID: 12482926, DOI: 10.1242/jcs.00215.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesApolipoprotein B-48Apolipoproteins BCells, CulturedChylomicronsCOP-Coated VesiclesEndoplasmic ReticulumEpithelial CellsGolgi ApparatusIntestinal AbsorptionIntestinal MucosaLipid MetabolismMaleMembrane FusionMicroscopy, ElectronMonomeric GTP-Binding ProteinsProtein TransportRatsSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsPre-chylomicron transport vesicleCOPII proteinsEndoplasmic reticulumTransport vesiclesBudding of vesiclesSucrose density centrifugationNascent proteinsCargo proteinsProtein vesiclesMembrane proteinsGolgi complexProteinase K treatmentProteinSec24VesiclesGolgiSar1Intestinal GolgiDensity centrifugationReticulumK treatmentTriton XSec13/31Rbet1COPII
1990
Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein.
Cohen ME, Reinlib L, Watson AJ, Gorelick F, Rys-Sikora K, Tse M, Rood RP, Czernik AJ, Sharp GW, Donowitz M. Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8990-8994. PMID: 2174171, PMCID: PMC55086, DOI: 10.1073/pnas.87.22.8990.Peer-Reviewed Original ResearchConceptsDependent protein kinase IIProtein kinase IIKinase IIMembrane proteinsSynapsin IDependent protein kinase activityDependent protein kinaseBrush border membranePhosphorylation of sitesProtein kinase activityInhibitor peptideCaM kinase IIBrush border membrane proteinsBorder membraneSpecific inhibitor peptidePhosphopeptide mappingBrush borderProtein kinaseMicrovillus membrane proteinsKinase activityIleal brush-border membraneVillus cell brush border membraneApical membraneKinaseVesicle preparations
1986
Phytohemagglutinin from red kidney bean (Phaseolus vulgaris) inhibits sodium and chloride absorption in the rabbit ileum
Dobbins J, Laurenson J, Gorelick F, Banwell J. Phytohemagglutinin from red kidney bean (Phaseolus vulgaris) inhibits sodium and chloride absorption in the rabbit ileum. Gastroenterology 1986, 90: 1907-1913. PMID: 3009261, DOI: 10.1016/0016-5085(86)90260-x.Peer-Reviewed Original ResearchConceptsRabbit ileumTissue conductanceCyclic guanosine monophosphateEffects of phytohemagglutininIndicator of absorptionAmino acid absorptionSerosal calciumMucosal levelSerosal additionCyclic adenosine monophosphatePhytohemagglutininControl tissuesChloride absorptionCrypt cellsAcid absorptionIleumRabbit colonDietary lectinsGuanosine monophosphateEpithelial surfaceVillus cellsElectrolyte transportLuminal borderNet NaMucosal solution