2013
Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion
Rivera-Molina F, Toomre D. Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion. Journal Of Cell Biology 2013, 201: 673-680. PMID: 23690179, PMCID: PMC3664709, DOI: 10.1083/jcb.201212103.Peer-Reviewed Original ResearchConceptsLive-cell imagingVesicle fusionEndocytic recycling compartmentAttachment protein receptorsSNARE fusion machineryExocyst complexMembrane traffickingFusion machineryRecycling compartmentCell cortexSec8Cell protrusionsPlasma membraneVesicle attachmentFusion poreMembrane expansionProtein receptorsCell polarizationFluorescence recoverySpatiotemporal dynamicsExocystUbiquitous roleVesiclesTraffickingMorphological criteria
2007
The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts
Destaing O, Sanjay A, Itzstein C, Horne WC, Toomre D, De Camilli P, Baron R. The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts. Molecular Biology Of The Cell 2007, 19: 394-404. PMID: 17978100, PMCID: PMC2174183, DOI: 10.1091/mbc.e07-03-0227.Peer-Reviewed Original ResearchConceptsKinase activityActin cloudDynamic actin-rich structuresSrc family kinase activityDynamics of podosomesActin-rich structuresF-actin coresTyrosine kinase activityAbsence of SrcSrc mutantActin fluxActin dynamicsPodosome numbersEssential regulatorLife spanPodosomesActin polymerizationPodosome organizationF-actinPodosome structuresSrc inhibitorSrcFluorescence recoveryPodosome beltSpecific functions