2012
Cellular imaging using total internal reflection fluorescence microscopy: theory and instrumentation.
Toomre D. Cellular imaging using total internal reflection fluorescence microscopy: theory and instrumentation. Cold Spring Harbor Protocols 2012, 2012: 414-24. PMID: 22474668, DOI: 10.1101/pdb.top068650.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyRefractive indexHigh numerical aperture objective lensNumerical aperture objective lensThinner optical planeDifferent refractive indicesDynamic cellular processesFluorescence microscopyCell fluorescent microscopyTransient intermediate statesConventional epifluorescence microscopeEvanescent fieldMembrane traffickingDeep imagingObjective lensCellular processesCytoskeleton remodelingCell cortexOptical planeCell signalingAxial resolutionOptical principlesOrganelle levelMolecular manipulation
2011
3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework
Yang Q, Karpikov A, Toomre D, Duncan JS. 3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework. IEEE Transactions On Image Processing 2011, 20: 2248-2259. PMID: 21324778, DOI: 10.1109/tip.2011.2114359.Peer-Reviewed Original ResearchConceptsEvanescent fieldDifferent penetration depthsTIRF imagesPenetration depthSamples of microtubulesLaser beamTotal internal reflection fluorescence microscopyAxial resolutionReflection fluorescence microscopyLarge radiusIncident angleMulti-angle total internal reflection fluorescence microscopyElectron microscopy imagesTIRF dataSmall radiusTracking of microtubulesMicroscopy imagesFluorescence microscopyMicroscopyRadiusReconstruction resultsCurvilinear characteristicsZ-dimensionExperimental calibrationMicrotubule curvature
2010
A New Wave of Cellular Imaging
Toomre D, Bewersdorf J. A New Wave of Cellular Imaging. Annual Review Of Cell And Developmental Biology 2010, 26: 285-314. PMID: 20929313, DOI: 10.1146/annurev-cellbio-100109-104048.Peer-Reviewed Original ResearchConceptsTransient intermediate statesDiffraction limitTotal internal reflection fluorescence microscopyNanoscopy techniquesEmission depletionStructured illuminationAxial resolutionOptical principlesNanometer resolutionUse of scanningIntermediate stateSingle moleculesCellular imagingReflection fluorescence microscopyBiological applications