Quantification of Biomolecular Dynamics Inside Real and Synthetic Nuclear Pore Complexes Using Time-Resolved Atomic Force Microscopy
Stanley GJ, Akpinar B, Shen Q, Fisher PDE, Lusk CP, Lin C, Hoogenboom BW. Quantification of Biomolecular Dynamics Inside Real and Synthetic Nuclear Pore Complexes Using Time-Resolved Atomic Force Microscopy. ACS Nano 2019, 13: 7949-7956. PMID: 31241896, PMCID: PMC6660115, DOI: 10.1021/acsnano.9b02424.Peer-Reviewed Original ResearchConceptsNuclear pore complexAtomic force microscopyTransport barrierForce microscopyBiomolecular dynamicsPore complexNative nuclear pore complexesDynamics of biomoleculesNanometer length scaleDNA origami scaffoldMs temporal resolutionCollective transitionsMacromolecular trafficSelective gatewaySuch proteinsBiomolecular systemsOrigami scaffoldCohesive interactionsObserved dynamicsSuch experimentsTime scalesProteinBiological systemsLength scalesDrift correction