2015
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis. Cell 2015, 160: 1246-1260. PMID: 25748654, PMCID: PMC4380877, DOI: 10.1016/j.cell.2015.02.038.Peer-Reviewed Original ResearchConceptsSingle guide RNAsGenome-wide CRISPR screenGenome-scale libraryCRISPR/Cas9-mediated lossLate-stage primary tumorsEffects of mutationsGenetic screenTumor growthCRISPR screensFunction screenMouse cancer cell linesCancer evolutionGene phenotypeDiverse phenotypesFunction mutationsCancer cell linesGenesCell linesPrimary screenCell poolSpecific lossPrimary tumor growthSmall poolMutationsPhenotype
2014
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell 2014, 159: 440-455. PMID: 25263330, PMCID: PMC4265475, DOI: 10.1016/j.cell.2014.09.014.Peer-Reviewed Original Research