John Greg Howe PhD

Associate Professor of Laboratory Medicine; Director, Molecular Diagnostics Laboratory

Research Interests

Molecular Diagnostics; Hematopathology

Research Summary

The mission of the Molecular Diagnostics Laboratory is to develop and bring into clinical use already published molecular pathology assays as well as to develop new and innovative molecular tests for the diagnosis of disease and the monitoring of therapy. Various tests have been developed and are in clinical use such as detection of mutations in the prothrombin and Factor V genes that are associated with coagulation abnormalities and tests to determine monoclonality in patients with suspected hematological malignancies. Previously, we have developed an in situ hybridization assay to test for the presence of Epstein-Barr virus (EBV) in fixed tissue and have used this test to study the role of EBV in several diseases. Using the in situ hybridization method in conjunction with flow cytometry we have been able to demonstrate the utility of a method to quantitate the number of EBV containing cells. We have applied quantitative polymerase chain reaction (QPCR) methods to the measurement of EBV viral load in allogenic transplant patients and have further refined the method with the development of a real-time quantitative EBV PCR assay.

Selected Publications

  • Ornstein, DL, Bifulco, CB, Braddock, DT and JG Howe. Histopathologic and Molecular Aspects of CD56+ NK/T-Cell Lymphoma of the Testis. International Journal of Surgical Pathology (2008). 16:291-300.
  • Ernst, LM, Crouch, J, Rinder, H, JG Howe (2005) Bacterial Etiology for Chronic Villitis is not supported by polymerase chain reaction for 16S rRNA DNA, Pediatric and Developmental Pathology, 8, 647-653
  • Rhoden KJ, Johnson C, Brandao G, Howe JG, Smith BR, Tallini G (Dec 2004) Real-time quantitative RT-PCR identifies distinct c-RET, RET/PTC1 and RET/PTC3 expression patterns in papillary thyroid carcinoma., Laboratory Investigation; a Journal of Technical Methods and Pathology, 84(12)1557-70
  • Wang S, Li N, Heald P, Fisk JM, Fadare O, Howe JG, McNiff JM, Smith BR (Nov 2004) Flow cytometric DNA ploidy analysis of peripheral blood from patients with sezary syndrome: detection of aneuploid neoplastic T cells in the blood is associated with large cell transformation in tissue., American Journal of Clinical Pathology, 122(5)774-82
  • Vielemeyer O, Crouch JY, Edberg SC, Howe JG, Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B., Journal of Clinical Microbiology, 42(2):847-9, 2004
  • Howe JG, Crouch J, Cooper D, Smith BR, Real-time quantitative reverse transcription-PCR for cyclin D1 mRNA in blood, marrow, and tissue specimens for diagnosis of mantle cell lymphoma, Clinical Chemistry, 50(1):80-7, 2004

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