Fluorescence-Activated Cell Sorting Facility

Our mission is to provide comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation.

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. Any suspended particle or cell from 0.5–150 micrometers in size is suitable for analysis. Cells from solid tissue must be disaggregated before analysis. Sorting allows us to capture and collect cells of interest for further analysis. Once collected, the cells can be analyzed microscopically, biochemically, or functionally.

To learn more, view Invitrogen's Introduction to Flow Cytometry tutorial.



This facility is co-sponsored by the Yale Cancer Center and the Yale Rheumatic Disease Research Core Center.

Location

FACS Facility is located:

  • The 6th floor of The Anlyan Center for Medical Research
    (TAC rooms S613, 617, 633, 533, 522)
  • Amistad building (in rooms 105 and 416)
  • LEPH (in room 901B)
  • Room 2320H at 300 George Street.

Flow Cytometry Facility News and Resources

Several improvements to the facility that are now available. 

  • Administration of a FlowJo site license for Yale.
  • Remote data access via Box@Yale. 
  • March 1-20th- Amistad FACSCalibur room 105 renovation in order to have more room to house both Calibur and Amistad LSRII. 
  • March 27-mid-April-Amistad room 416 renovation and addition of a Baker BioProtect IV Safety cabinet.
  • In an effort to better support translational research, the Amistad and LEPH sorters will be both be upgraded  to create equivalent laser configurations.