2013
Asn441 plays a key role in folding and function of the Na+/I– symporter (NIS)
Li W, Nicola JP, Amzel LM, Carrasco N. Asn441 plays a key role in folding and function of the Na+/I– symporter (NIS). The FASEB Journal 2013, 27: 3229-3238. PMID: 23650190, PMCID: PMC3714583, DOI: 10.1096/fj.13-229138.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAsparagineBinding SitesBiological TransportCell LineCell MembraneChlorocebus aethiopsCOS CellsHumansImmunoblottingIodineMicroscopy, ConfocalModels, MolecularMolecular Sequence DataMutationProtein FoldingProtein Structure, SecondaryProtein Structure, TertiarySequence Homology, Amino AcidSymportersTransport Vesicles
2007
Amino Acid Residues in Transmembrane Segment IX of the Na+/I– Symporter Play a Role in Its Na+ Dependence and Are Critical for Transport Activity*
De la Vieja A, Reed MD, Ginter CS, Carrasco N. Amino Acid Residues in Transmembrane Segment IX of the Na+/I– Symporter Play a Role in Its Na+ Dependence and Are Critical for Transport Activity*. Journal Of Biological Chemistry 2007, 282: 25290-25298. PMID: 17606623, DOI: 10.1074/jbc.m700147200.Peer-Reviewed Original ResearchConceptsMembrane/cytosol interfaceActive I(-) transportAmino acid residuesPlasma membrane glycoproteinsBinding/translocationProtein familySegment IXNIS mutationPosition 354Asp-369NIS functionAcid residuesTransport activityMembrane glycoproteinsAmino acidsFunctional significanceResiduesSymporterI- transportKey roleSide chainsTranslocationTransportersThrHelix