2024
Therapeutic potential of extracellular vesicles from diverse sources in cancer treatment
Lin H, Zhou J, Ding T, Zhu Y, Wang L, Zhong T, Wang X. Therapeutic potential of extracellular vesicles from diverse sources in cancer treatment. European Journal Of Medical Research 2024, 29: 350. PMID: 38943222, PMCID: PMC11212438, DOI: 10.1186/s40001-024-01937-x.Peer-Reviewed Original ResearchConceptsPotential of extracellular vesiclesExtracellular vesiclesTumor growthDissemination of primary tumorsCancer treatmentBiological characteristics of extracellular vesiclesTherapeutic potential of extracellular vesiclesRegulate tumor growthEffective cancer treatmentCharacteristics of extracellular vesiclesCytoplasmic proteinsEngineered extracellular vesiclesPrimary tumorComplex diseasesDistant organsCancer therapyCancer developmentCell differentiationImmune responseInfluence cancerCancerTherapeutic potentialIntercellular communicationExcessive proliferationOncogenic factorResearch progress on astrocyte-derived extracellular vesicles in the pathogenesis and treatment of neurodegenerative diseases
Zhu Y, Wang F, Xia Y, Wang L, Lin H, Zhong T, Wang X. Research progress on astrocyte-derived extracellular vesicles in the pathogenesis and treatment of neurodegenerative diseases. Reviews In The Neurosciences 2024, 0 PMID: 38889403, DOI: 10.1515/revneuro-2024-0043.Peer-Reviewed Original ResearchAstrocyte-derived extracellular vesiclesProteins associated with neurodegenerative diseasesTreatment of neurodegenerative diseasesExtracellular vesiclesNeurodegenerative disordersNeurodegenerative diseasesHuntington's diseaseAggregation of pathogenic proteinsAlzheimer's diseaseTransfer of biomoleculesAmyotrophic lateral sclerosisCell to cellDiverse target cellsIntercellular communicationPathogenic proteinsContext of neurological disordersIntercellular transportAberrant accumulationPotential dual roleCentral nervous systemDevelopment of neurological disordersVesiclesProteinLateral sclerosisNeurological disordersRole of mesenchymal stem cell-derived exosomes in the regeneration of different tissues
Huang D, Shen H, Xie F, Hu D, Jin Q, Hu Y, Zhong T. Role of mesenchymal stem cell-derived exosomes in the regeneration of different tissues. Journal Of Biological Engineering 2024, 18: 36. PMID: 38845032, PMCID: PMC11155050, DOI: 10.1186/s13036-024-00431-6.Peer-Reviewed Original ResearchMesenchymal stem cellsMSC-ExosTissue regenerationMesenchymal stem cell-derived exosomesStem cell-derived exosomesImmune effector cellsCell-derived exosomesIschemic heart diseaseMSC-derived exosomesEffector cellsLung injuryArticular cartilageTissue applicationsLiver fibrosisInflammatory diseasesImmune responseHeart diseaseAnti-inflammatory compoundsStem cellsIntercellular communicationClinical applicationDiseaseExosomesTissueLiverExtracellular vesicles containing MFGE8 from colorectal cancer facilitate macrophage efferocytosis
Ma Z, Sun Y, Yu Y, Xiao W, Xiao Z, Zhong T, Xiang X, Li Z. Extracellular vesicles containing MFGE8 from colorectal cancer facilitate macrophage efferocytosis. Cell Communication And Signaling 2024, 22: 295. PMID: 38802814, PMCID: PMC11131254, DOI: 10.1186/s12964-024-01669-9.Peer-Reviewed Original ResearchConceptsProteomic analysisExtracellular vesiclesSignaling pathwayCRC cell linesFlow cytometric analysisMacrophage efferocytosisCRC cellsClear apoptotic cellsCell linesMass spectrometry-based proteomic analysisCRISPR-Cas9 gene editing systemCytometric analysisInvolvement of extracellular vesiclesEfferocytosis in vitroCancer Genome AtlasGene editing systemTumor microenvironmentCRISPR-Cas9Apoptotic cellsCell surfacePromotion of drug resistanceMFGE8Genome AtlasProtein moleculesEditing systemMicrobial extracellular vesicles contribute to antimicrobial resistance
Jiang B, Lai Y, Xiao W, Zhong T, Liu F, Gong J, Huang J. Microbial extracellular vesicles contribute to antimicrobial resistance. PLOS Pathogens 2024, 20: e1012143. PMID: 38696356, PMCID: PMC11065233, DOI: 10.1371/journal.ppat.1012143.Peer-Reviewed Original ResearchConceptsMicrobial extracellular vesiclesAntimicrobial resistanceExtracellular vesiclesBacterial extracellular vesiclesDrug-resistant microbesAntimicrobial resistance crisisParasite extracellular vesiclesResistance crisisGlobal antimicrobial resistance crisisAntimicrobial approachesAntimicrobial therapyVesiclesFungalMicrobesResistanceTumor-derived extracellular vesicles regulate macrophage polarization: role and therapeutic perspectives
Wang L, Wang W, Hu D, Liang Y, Liu Z, Zhong T, Wang X. Tumor-derived extracellular vesicles regulate macrophage polarization: role and therapeutic perspectives. Frontiers In Immunology 2024, 15: 1346587. PMID: 38690261, PMCID: PMC11058222, DOI: 10.3389/fimmu.2024.1346587.Peer-Reviewed Original ResearchConceptsTumor-derived extracellular vesiclesTumor developmentBreast carcinomaLung carcinomaExtracellular vesiclesM2 phenotypeMacrophage polarizationM2 polarizationPromote tumor developmentStimulate macrophage polarizationAnti-tumor activityM2 to M1 macrophagesAnti-inflammatory effectsInduced M2 polarizationPancreatic carcinomaPro-tumorColorectal carcinomaTumor progressionHepatocellular carcinomaCarcinomaColorectal cancerRegulating tumor developmentCell ratioM1 macrophagesPromote M2 polarizationResearch progress on ferroptosis in the pathogenesis and treatment of neurodegenerative diseases
Wang L, Fang X, Ling B, Wang F, Xia Y, Zhang W, Zhong T, Wang X. Research progress on ferroptosis in the pathogenesis and treatment of neurodegenerative diseases. Frontiers In Cellular Neuroscience 2024, 18: 1359453. PMID: 38515787, PMCID: PMC10955106, DOI: 10.3389/fncel.2024.1359453.Peer-Reviewed Original ResearchNeurodegenerative diseasesHuntington's diseaseAlzheimer's diseaseAmyotrophic lateral sclerosisSignaling networksDisease-related researchTreatment of neurodegenerative diseasesCell deathPathogenic cascadeIron-dependentNeurodegenerative disordersBreakdown of cellsNeurodegenerative conditionsFerroptosisLateral sclerosisNeuronal lossParkinson's diseaseCellsHuntingtonTherapeutic approachesAlzheimerLipid peroxidationDiseaseMechanismLipidOxymatrine Inhibition of Hepatitis B Virus Replication Through ERK1/2 Pathway and HNF1α and HNF4α Block <em>in vitro</em>.
Wang F, Wang X, Ling B, Lu H, Huang J, Zhong T. Oxymatrine Inhibition of Hepatitis B Virus Replication Through ERK1/2 Pathway and HNF1α and HNF4α Block in vitro. Journal Of College Of Physicians And Surgeons Pakistan 2024, 34: 329-335. PMID: 38462870, DOI: 10.29271/jcpsp.2024.03.329.Peer-Reviewed Original ResearchConceptsTranscription factor HNF1AHepatitis B virusDNA copy numberHNF4A expressionCell-free culture mediumInhibition of hepatitis B virus replicationProtein translationRNA gene expressionDNA replicationHepatitis B virus DNA replicationHepatitis B virus DNAPhosphorylation of ERK1/2Copy numberGene transcriptionReal-time PCRERK1/2 protein expressionB virus replicationGene expressionReplication of hepatitis B virusEnzyme-linked immunosorbent assay kitHepatitis B virus replicationCell-based modelsSignaling factorsHBV DNA copy numberMolecular mechanismsGlycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis
Guo Q, Zhao M, Wang Q, Lu T, Luo P, Chen L, Xia F, Pang H, Shen S, Cheng G, Dai C, Meng Y, Zhong T, Qiu C, Wang J. Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis. Biomedicine & Pharmacotherapy 2024, 173: 116304. PMID: 38401519, DOI: 10.1016/j.biopha.2024.116304.Peer-Reviewed Original ResearchNon-small cell lung cancerCell lung cancerMitochondrial apoptosisActivity-based protein profilingLung cancerTreatment of non-small cell lung cancerGlycyrrhetinic acidInhibit non-small cell lung cancerCombination of histopathologyCaspase-3Proteomic validationTarget of GAIn vivo resultsProtein profilesPeroxiredoxin 6Clinical applicationIn vitroApoptosisPotential targetCancerPrdx6Application of GAPromoterResearch progress of CTC, ctDNA, and EVs in cancer liquid biopsy
Wang X, Wang L, Lin H, Zhu Y, Huang D, Lai M, Xi X, Huang J, Zhang W, Zhong T. Research progress of CTC, ctDNA, and EVs in cancer liquid biopsy. Frontiers In Oncology 2024, 14: 1303335. PMID: 38333685, PMCID: PMC10850354, DOI: 10.3389/fonc.2024.1303335.Peer-Reviewed Original ResearchCirculating tumor DNAExtracellular vehiclesLiquid biopsyTumor-derived materialCirculating cancer cellsLiquid biopsy biomarkersOriginal tumorTumor DNATumor cellsClinical trialsPrognosis of neoplastic diseasesCancer cellsPrognosis predictionTherapeutic monitoringClinical utilityNeoplastic diseaseBiopsyTumorPrecision medicinePrognosisCancer diagnosisBiomarkersCancerDiagnosisCellsSmall extracellular vesicles promote the formation of the pre-metastatic niche through multiple mechanisms in colorectal cancer
Wu J, Wang X, Li Z, Yi X, Hu D, Wang Q, Zhong T. Small extracellular vesicles promote the formation of the pre-metastatic niche through multiple mechanisms in colorectal cancer. Cell Cycle 2024, 23: 131-149. PMID: 38341861, PMCID: PMC11037293, DOI: 10.1080/15384101.2024.2311501.Peer-Reviewed Original ResearchPre-metastatic nicheEpithelial-to-mesenchymal transitionSmall extracellular vesiclesPre-metastatic niche formationExtracellular vesiclesStromal cell activationPromote immunosuppressionPromote lymphangiogenesisMetastatic potentialColorectal cancerVascular permeabilityMetastatic invasionCell activationInflammatory factorsGlobal malignancyUpregulated inflammatory factorsMultiple mechanismsPotential mechanismsRemodelingNetwork remodeling
2023
ZIP14 Affects the Proliferation, Apoptosis, and Migration of Cervical Cancer Cells by Regulating the P38 MAPK Pathway
Jiang L, Xie T, Xia Y, Li F, Zhong T, Lai M. ZIP14 Affects the Proliferation, Apoptosis, and Migration of Cervical Cancer Cells by Regulating the P38 MAPK Pathway. Current Cancer Drug Targets 2023, 24: 779-790. PMID: 37990424, DOI: 10.2174/0115680096250711231024063841.Peer-Reviewed Original ResearchMolecular underpinningsP38 MAPK pathway inhibitor SB203580Mitogen-activated protein kinase pathwayP38 mitogen-activated protein kinase (MAPK) pathwayCervical cancerTarget gene identificationCC cellsKEGG pathway analysisProtein kinase pathwayGene Expression OmnibusP38 MAPK pathwayProgression of CCGene identificationTarget genesMolecular basisKinase pathwayPathogenesis of CCInhibitor SB203580Pathway analysisCC cell migrationMAPK pathwayMajor public health concernVivo tumorigenesisCervical cancer cellsTumorigenesis assaysCharacteristics of hearing loss-associated gene mutations: A multi-center study of 119,606 neonates in Gannan
Zhao M, Luo X, Zhao Q, Yang T, Zhang W, Chen Z, Zeng S, Chen W, Zhang H, Wang Q, Wang W, Zhang X, Zhong T. Characteristics of hearing loss-associated gene mutations: A multi-center study of 119,606 neonates in Gannan. International Journal Of Pediatric Otorhinolaryngology 2023, 174: 111744. PMID: 37801830, DOI: 10.1016/j.ijporl.2023.111744.Peer-Reviewed Original ResearchConceptsMulti-center studyHearing screeningOtoacoustic emissionsGenetic screeningHereditary HLPrevalent variantCommon congenital disabilityGene mutationsAuditory brainstem responseCongenital HLConcurrent hearingRisk factorsBrainstem responseDetection rateHigh riskHigh incidenceIdentification of individualsEducational sequelaeGJB2 c.NeonatesSLC26A4 c.Gene variantsCongenital disabilitiesSerious burdenMolecular etiology
2021
The Essential Function of SETDB1 in Homologous Chromosome Pairing and Synapsis during Meiosis
Cheng EC, Hsieh CL, Liu N, Wang J, Zhong M, Chen T, Li E, Lin H. The Essential Function of SETDB1 in Homologous Chromosome Pairing and Synapsis during Meiosis. Cell Reports 2021, 34: 108575. PMID: 33406415, PMCID: PMC8513770, DOI: 10.1016/j.celrep.2020.108575.Peer-Reviewed Original ResearchConceptsEarly meiosisEarly meiotic prophase IFunction of SETDB1Homologous chromosome pairingMeiotic prophase IHistone-lysine N-methyltransferaseMeiotic silencingSurvival of spermatocytesGermline developmentBouquet formationHomologous chromosomesLineage genesChromosome pairingBivalent formationPericentromeric regionProphase IApoptosis of spermatocytesSETDB1Essential functionsHomologous bivalentsH3K9me3Meiotic arrestMeiosisSpermatocytesN-methyltransferase
2020
Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons
Xiang Y, Tanaka Y, Patterson B, Hwang SM, Hysolli E, Cakir B, Kim KY, Wang W, Kang YJ, Clement EM, Zhong M, Lee SH, Cho YS, Patra P, Sullivan GJ, Weissman SM, Park IH. Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons. Molecular Cell 2020, 79: 84-98.e9. PMID: 32526163, PMCID: PMC7375197, DOI: 10.1016/j.molcel.2020.05.016.Peer-Reviewed Original ResearchConceptsMECP2 mutant neuronsEnhancer-promoter interactionsRett syndromeRTT-like phenotypesChromatin bindingMeCP2 functionMethyl-CpGAbnormal transcriptionRTT etiologyMutant neuronsBET inhibitorsPotential therapeutic opportunitiesMECP2 mutationsProtein 2Human brain organoidsFunctional phenotypeJQ1BRD4Therapeutic opportunitiesBrain organoidsFunction underliesMutationsPhenotypeHuman brain culturesCritical driverPublisher Correction: MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors
Chen X, Burkhardt DB, Hartman AA, Hu X, Eastman AE, Sun C, Wang X, Zhong M, Krishnaswamy S, Guo S. Publisher Correction: MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors. Nature Communications 2020, 11: 681. PMID: 31996673, PMCID: PMC6989496, DOI: 10.1038/s41467-020-14428-4.Peer-Reviewed Original Research
2019
MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors
Chen X, Burkhardt DB, Hartman AA, Hu X, Eastman AE, Sun C, Wang X, Zhong M, Krishnaswamy S, Guo S. MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors. Nature Communications 2019, 10: 5767. PMID: 31852898, PMCID: PMC6920141, DOI: 10.1038/s41467-019-13666-5.Peer-Reviewed Original ResearchAnimalsCell CycleCell DifferentiationCell ProliferationCell Transformation, NeoplasticCyclin D1Disease Models, AnimalFemaleGene Expression Regulation, LeukemicGene Knock-In TechniquesHumansKaplan-Meier EstimateLeukemia, Myeloid, AcuteMaleMice, TransgenicMyeloid Progenitor CellsMyeloid-Lymphoid Leukemia ProteinOncogene Proteins, FusionPiperazinesPrimary Cell CulturePrognosisPyridinesEngineering of human brain organoids with a functional vascular-like system
Cakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Kang YJ, Chapeton K, Patterson B, Yuan Y, He CS, Raredon MSB, Dengelegi J, Kim KY, Sun P, Zhong M, Lee S, Patra P, Hyder F, Niklason LE, Lee SH, Yoon YS, Park IH. Engineering of human brain organoids with a functional vascular-like system. Nature Methods 2019, 16: 1169-1175. PMID: 31591580, PMCID: PMC6918722, DOI: 10.1038/s41592-019-0586-5.Peer-Reviewed Original ResearchConceptsHuman cortical organoidsBlood-brain barrier characteristicsTrans-endothelial electrical resistanceVasculature-like structuresHuman brain organoidsHuman brain developmentCortical organoidsFunctional maturationPrenatal brainBrain diseasesBrain developmentHuman embryonic stem cellsBlood vesselsBrain organoidsTight junctionsDiseaseStem cellsOrganoidsVariant 2Nutrient transportersNutrient deliveryCellsEndotheliumMicrovasculatureMKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation
Hu X, Liu ZZ, Chen X, Schulz VP, Kumar A, Hartman AA, Weinstein J, Johnston JF, Rodriguez EC, Eastman AE, Cheng J, Min L, Zhong M, Carroll C, Gallagher PG, Lu J, Schwartz M, King MC, Krause DS, Guo S. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nature Communications 2019, 10: 1695. PMID: 30979898, PMCID: PMC6461646, DOI: 10.1038/s41467-019-09636-6.Peer-Reviewed Original ResearchConceptsCell fate reprogrammingChromatin accessibilityActin cytoskeletonSomatic cell reprogrammingPluripotency transcription factorsGlobal chromatin accessibilityGenomic accessibilityCytoskeleton (LINC) complexCell reprogrammingCytoskeletal genesTranscription factorsReprogrammingPluripotencyChromatinCytoskeletonMKL1Unappreciated aspectPathwayNuclear volumeNucleoskeletonSUN2CellsActivationGenesExpressionhESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids
Xiang Y, Tanaka Y, Cakir B, Patterson B, Kim KY, Sun P, Kang YJ, Zhong M, Liu X, Patra P, Lee SH, Weissman SM, Park IH. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids. Cell Stem Cell 2019, 24: 487-497.e7. PMID: 30799279, PMCID: PMC6853597, DOI: 10.1016/j.stem.2018.12.015.Peer-Reviewed Original ResearchConceptsReciprocal projectionsThree-dimensional organoid modelsForebrain disorderHuman brain developmentCortical organoidsHuman thalamusPeripheral tissuesThalamusRelated disordersThalamic developmentSingle-cell RNA sequencingBrain developmentHuman embryonic stem cellsCircuit organizationCortexOrganoid modelsRegion-specific organoidsTelencephalic fateStem cellsOrganoid techniquesOrganoidsDisordersRNA sequencingRelay hubDisease