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Postdoctoral Fellow
Biography
Arthur Stem received his Ph.D. in Toxicology from the University of Colorado Anschutz Medical Campus. His dissertation centered on contributions of environmental toxicants to the pathogenesis of chronic kidney disease of unknown etiology. He joined the Vasilis lab in 2024 and is using his background in environmental and mechanistic toxicology to elucidate how environmental exposures influence the development of liver disease.
Last Updated on February 07, 2025.
Education & Training
- PhD
- University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences, Toxicology (2024)
- BS
- University of Arkansas at Little Rock, Biology (2014)
- AS
- National Park Community College, Science (2011)
Research
Publications
2026
Quantitative Spatial Analysis of PFAS in Nine Biological Tissues from Mouse via MALDI-TIMS-MSI
Nikolopoulou V, Stem A, Alayyoub M, Vasiliou V, Aalizadeh R. Quantitative Spatial Analysis of PFAS in Nine Biological Tissues from Mouse via MALDI-TIMS-MSI. Environmental Science And Technology 2026, 60: 16419-16434. PMID: 42219700, DOI: 10.1021/acs.est.5c14803.Peer-Reviewed Original ResearchConceptsmass spectrometry imagingMatrix-Assisted Laser Desorption Ionizationion mobility separationmobility separationionization efficiencymatrix-assisted laser desorption ionization matriceslaser desorption ionizationanalysis of PFASseffects of ammonium fluoridepolyfluoroalkyl substancesdesorption ionizationin situ analytical methodsionization agentsammonium fluorideionizationquantitative spatial analysisanalytical methodseparationadverse health effectschemicalionshigh-throughputsynthetic chemicalswidespread classhealth effects23 Interaction between sugarcane ash-derived silica nanoparticles and pesticides leads to mitochondrial impairment in human proximal tubular kidney cells
Chesterman C, Stem A, Anderson C, Brown J. 23 Interaction between sugarcane ash-derived silica nanoparticles and pesticides leads to mitochondrial impairment in human proximal tubular kidney cells. Annals Of Work Exposures And Health 2026, 70: wxag024.081. DOI: 10.1093/annweh/wxag024.081.Peer-Reviewed Original Researchproximal convoluted tubuleskidney biopsychronic kidney disease of unknown etiologyexposure to silica nanoparticlesdisease of unknown etiologydose-dependent mannertreated cellsSeahorse XF Analyzerconcentration-dependent mannerparticulate matterproximal tubular kidney cellsunknown etiologyPCT cellssugarcane ashtubular kidney cellsconvoluted tubuleskidney damagemitochondrial functionHK-2levels of pesticidesoxygen consumption rateamount of pesticideskidney cellsurinekidneyIntegrated Multi-Omics Reveals Synergistic Hepatotoxicity of Ethanol and PFOS Co-Exposure
Stem A, Alayyoub M, Aalizadeh R, Nikolopoulou V, Lisgara A, Shvartsman A, Anitha M, Patterson A, Coble R, Rushing B, Sumner S, Vasiliou V. Integrated Multi-Omics Reveals Synergistic Hepatotoxicity of Ethanol and PFOS Co-Exposure. Chemico-Biological Interactions 2026, 434: 112101. PMID: 42036057, DOI: 10.1016/j.cbi.2026.112101.Peer-Reviewed Original ResearchConceptsalcohol-associated liver diseasesusceptible to alcohol-induced liver injuryalcohol-induced liver injurysystemic metabolic disturbancesexposure to perfluorooctanesulfonic acidfatty acid b-oxidationLieber-DeCarli modelbile acid transporterimpaired lipid handlingmechanism of hepatotoxicityconcurrent ethanolpotential modifiersethanol intakeplasma metabolomesynergistic hepatotoxicityinjury-related responsesmetabolomic profileshepatocellular injurycommensal anaerobesmetabolic disturbancesliver injurygut-liverliver diseaseco-exposuremicrovesicular steatosisExposure to Per- and Polyfluoroalkyl Substances and Liver Cancer: A Systematic Review of Animal and Epidemiological Studies
Tanghal R, Beglarian E, Stem A, Aung M, Alderete T, Ducatman A, Vasiliou V, McConnell R, Conti D, Chatzi L. Exposure to Per- and Polyfluoroalkyl Substances and Liver Cancer: A Systematic Review of Animal and Epidemiological Studies. Environmental Science And Technology 2026, 60: 2816-2831. PMID: 41543329, PMCID: PMC12875370, DOI: 10.1021/acs.est.5c05535.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptssystematic reviewliver cancermortality rates of liver cancerlifestyle risk factorsrate of liver cancerepidemiological studiesliver cancer riskpublic health policiesanimal studiesmortality ratehuman epidemiologic findingshepatocarcinogenic effectshealth policycancer riskliver tumor incidencereview of animalinclusion criteriaepidemiological evidencetumor incidenceprospective studyexposure to per-liver toxicitypositive associationenvironmental exposuresrisk factors
2025
Investigating the Quantitative Structure–Ionization Efficiency Relationship of Small Molecules and Lipids in the Presence of Ammonium Fluoride in MALDI-TIMS-QTOF Mass Spectrometry Imaging
Nikolopoulou V, Stem A, Vasiliou V, Aalizadeh R. Investigating the Quantitative Structure–Ionization Efficiency Relationship of Small Molecules and Lipids in the Presence of Ammonium Fluoride in MALDI-TIMS-QTOF Mass Spectrometry Imaging. Journal Of The American Society For Mass Spectrometry 2025, 36: 2460-2469. PMID: 41147643, DOI: 10.1021/jasms.5c00211.Peer-Reviewed Original ResearchConceptsionization efficiencyammonium fluoridepresence of ammonium fluoridemass spectrometry imagingsmall moleculesisotopically labeled metabolitesnegative polarity modeanalysis of analytesendogenous compoundschemical structureintermediate productsionizationcompoundsmoleculesrelationship modelfluorideMALDImetabolitesanalytescomatrixquantitationmatrixpolarization modesstructuredihydrochlorideSynergistic toxicity in alcohol-associated liver disease and PFAS exposure
Stem A, Tieghi R, Chatzi V, Kleinstreuer N, Valvi D, Thompson D, Vasiliou V. Synergistic toxicity in alcohol-associated liver disease and PFAS exposure. Toxicological Sciences 2025, 208: 9-31. PMID: 40737496, PMCID: PMC12599875, DOI: 10.1093/toxsci/kfaf110.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statementsalcohol-associated liver diseaseliver diseasechronic ethanol intakecentral mechanismshepatotoxic effectspolyfluoroalkyl substanceshigh-risk populationoxidative stressdysregulated lipid metabolismethanol intakeliver functionliver injuryacetaldehyde-induced cytotoxicityhepatic functionhepatocellular damageoxidative stress inductionliver pathologyglobal morbiditypathogenic pathwayspolyfluoroalkyl substances exposuresexposure to polyfluoroalkyl substancesfatty acid oxidationhealthcare accesseffects of polyfluoroalkyl substancesmultiple mechanismsIntegrating gut microbiome and neuroplasticity genomics in alcohol use disorder therapy
Koutromanos I, Legaki E, Dovrolis N, Vassilopoulos E, Stem A, Vasiliou V, Tzavellas E, Gazouli M. Integrating gut microbiome and neuroplasticity genomics in alcohol use disorder therapy. Human Genomics 2025, 19: 78. PMID: 40646629, PMCID: PMC12255058, DOI: 10.1186/s40246-025-00793-y.Peer-Reviewed Original ResearchConceptsgut microbiotashort-chain fatty acid (SCFA)-producing bacteriametabolic pathwaysstress-related metabolic pathwayshost-microbiota interactionsmicrobial metabolic pathwaysmulti-omics approachgut barrier integritygene expression changesgene expression profilesneuroplasticity-related genesrRNA sequencinggut microbiomefamily genesgenomic signaturesmicrobiome compositionmicrobial compositionAUD patientsmicrobial dysbiosistreatment responsefunctional pathwaysgene expressionmicrobiomegenesexpression changesAdvancing translational exposomics: bridging genome, exposome and personalized medicine
Sarigiannis D, Karakitsios S, Anesti O, Stem A, Valvi D, Sumner S, Chatzi L, Snyder M, Thompson D, Vasiliou V. Advancing translational exposomics: bridging genome, exposome and personalized medicine. Human Genomics 2025, 19: 48. PMID: 40307849, PMCID: PMC12044731, DOI: 10.1186/s40246-025-00761-6.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsexposome-wide association studybridge genomicslifestyle exposuresenhancing causal inferencepublic health decision-makingenvironmental health researchhealth decision-makingmulti-omics technologiesgenomic variationgenomic dataassociation studieshealth outcomesbioinformatics approachhealth researchprecision preventiongenetic variabilityexposome dataexposure-response relationshipMulti-Omicsgenomeinternal exposomevulnerable populationscomplex diseasesdisease phenotypepublic healthcKit Ligand (Stem Cell Factor)-Induced Immune Response via an Alternative Receptor in Human Cells
Hwang J, Reinert A, Stem A, Shim S, Kim S, Kim H, Yoon M, Lee S, Song D, Yoo H, Han S, Choi M, Brown J, Kim S. cKit Ligand (Stem Cell Factor)-Induced Immune Response via an Alternative Receptor in Human Cells. Journal Of Inflammatory And Infectious Medicine 2025, 5. DOI: 10.53941/jiim.2025.100005.Peer-Reviewed Original ResearchHMC-1 cellsHMC-1human THP-1 monocytic cellshematopoietic stem cellsstem cell factormast cell growthalternative receptorsTHP-1 monocytic cellsIL-8stem 2cell growthhuman cellsbone marrow hematopoietic stem cellsmouse immune cellsimmune responsemarrow hematopoietic stem cellschemokine interleukin-8inflammatory chemokine interleukin-8human keratinocyte HaCaT cellsimmunological activityhuman IL-8keratinocyte HaCaT cellsmonocytic cellsproduction of inflammatory moleculescell factorModulation of the thiol redox proteome by sugarcane ash-derived silica nanoparticles: insights into chronic kidney disease of unknown etiology
Stem A, Michel C, Harris P, Rogers K, Gibb M, Roncal-Jimenez C, Reisdorph R, Johnson R, Roede J, Fritz K, Brown J. Modulation of the thiol redox proteome by sugarcane ash-derived silica nanoparticles: insights into chronic kidney disease of unknown etiology. Particle And Fibre Toxicology 2025, 22: 3. PMID: 39910563, PMCID: PMC11800628, DOI: 10.1186/s12989-025-00619-8.Peer-Reviewed Original ResearchMeSH KeywordsBasic Helix-Loop-Helix Transcription FactorsCell LineChronic Kidney Diseases of Uncertain EtiologyHumansKidney Tubules, ProximalNanoparticlesOccupational ExposureOxidation-ReductionOxidative StressProteomeProteomicsReactive Oxygen SpeciesReceptors, Aryl HydrocarbonRenal Insufficiency, ChronicSaccharumSilicon DioxideSulfhydryl CompoundsConceptsproximal convoluted tubulesactivation of aryl hydrocarbon receptorexposure to silica nanoparticlesdisease of unknown etiologyreactive oxygen specieskidney proximal convoluted tubuleincreased reactive oxygen species generationchronic kidney diseasesignaling pathwayIntroductionChronic kidney diseaseHK-2 cellsreactive oxygen species generationprofile in vitropotential mechanisms of toxicitymembrane hyperpolarizationCKD progressionunknown etiologygeneration of reactive oxygen speciesmitochondrial membrane hyperpolarizationoxygen species generationaryl hydrocarbon receptorenergy metabolismkidney diseaseconvoluted tubulesmechanisms of toxicity
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