2017
Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells
Whirledge S, Kisanga EP, Taylor RN, Cidlowski JA. Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells. Endocrinology 2017, 158: 4076-4092. PMID: 28938408, PMCID: PMC5695839, DOI: 10.1210/en.2017-00361.Peer-Reviewed Original ResearchConceptsPioneer factor FOXA1Polymerase 2Molecular mechanismsRecruitment of GRThousands of genesExpression of lefty1GR-dependent inductionGene expression analysisHuman endometrial cellsGlucocorticoid receptorGlucocorticoid-mediated inductionEndometrial cellsRegulatory regionsGlucocorticoid receptor signalingMaster regulatorSpecific genesExpression analysisPrimary human endometrial cellsGene expressionHuman uterine cellsGlucocorticoid-dependent inductionIntracellular signalingEmbryo implantationFOXA2Receptor recruitmentGenerating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor
Whirledge SD, Jewell CM, Barber LM, Xu X, Katen KS, Garantziotis S, Cidlowski JA. Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor. Laboratory Investigation 2017, 97: 1282-1295. PMID: 28759007, PMCID: PMC5759773, DOI: 10.1038/labinvest.2017.76.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdultAmino Acid SubstitutionBlack PeopleCell Line, TumorCells, CulturedCohort StudiesFemaleGene Expression RegulationGenetic Association StudiesGlucocorticoidsHispanic or LatinoHumansMacrophagesMaleNorth CarolinaPolymorphism, Single NucleotideProspective StudiesReceptors, GlucocorticoidRecombinant ProteinsRegistriesSignal TransductionWhite PeopleConceptsGene expression profilesGene expressionExpression profilesGlucocorticoid sensitivityT polymorphismWide gene expressionPrimary macrophagesInherent transcriptional activityHigher serum cortisol levelsLow-dose dexamethasoneProspective cohort studyProtein expressionSelf-reported allergiesDominant negative regulatorSerum cortisol levelsReceptor protein expressionSelf-reported diseasesGlucocorticoid receptor geneHuman glucocorticoid receptor geneMinor allele frequencyNon-Hispanic participantsAlternative splicingMRNA stabilityReceptor stabilityTranscriptional activity
2013
Global Gene Expression Analysis in Human Uterine Epithelial Cells Defines New Targets of Glucocorticoid and Estradiol Antagonism1
Whirledge S, Xu X, Cidlowski JA. Global Gene Expression Analysis in Human Uterine Epithelial Cells Defines New Targets of Glucocorticoid and Estradiol Antagonism1. Biology Of Reproduction 2013, 89: 66, 1-17. PMID: 23843231, PMCID: PMC4435104, DOI: 10.1095/biolreprod.113.111054.Peer-Reviewed Original ResearchConceptsImmune systemUterine endometrial cancer cell lineUterine epitheliumEndometrial cancer cell linesHuman uterine epithelial cellsMaintenance of pregnancyEffects of glucocorticoidsImmune cell traffickingSynthetic glucocorticoid dexamethasoneCell linesEpithelial-like cell lineFemale reproductive systemPresence of DexGlucocorticoid-regulated genesIngenuity Pathway AnalysisUterine epithelial cellsCancer cell linesInflammatory responseReproductive hormonesEstrogen receptorGlucocorticoid signalingEmbryo implantationGlucocorticoid dexamethasoneGlucocorticoid receptorGlucocorticoids