2015
Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding
Kayisli O, Kayisli UA, Basar M, Semerci N, Schatz F, Lockwood CJ. Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding. PLOS ONE 2015, 10: e0137855. PMID: 26436918, PMCID: PMC4593551, DOI: 10.1371/journal.pone.0137855.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsMedroxyprogesterone acetateGuinea pigsUterine bleedingCultured human endometrial stromal cellsStromal cellsGlandular cellsProgestin only contraceptivesEndometrial blood flowOvariectomized guinea pigsMajor side effectsEffective family planning methodsGlucocorticoid receptor activationGR transcriptional activityFragile blood vesselsFamily planning methodsIngenuity Pathway AnalysisFKBP51 expressionFunctional withdrawalMicroarray resultsOnly contraceptivesGlucocorticoid withdrawalEndometrial inflammationEffects of tibolone and its metabolites on prolactin and insulin-like growth factor binding protein-1 expression in human endometrial stromal cells
Guzel E, Buchwalder L, Basar M, Kayisli U, Ocak N, Bozkurt I, Lockwood CJ, Schatz F. Effects of tibolone and its metabolites on prolactin and insulin-like growth factor binding protein-1 expression in human endometrial stromal cells. Gynecological Endocrinology 2015, 31: 414-418. PMID: 25856298, DOI: 10.3109/09513590.2015.1014788.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternContraceptive Agents, FemaleDexamethasoneEndometriumEnzyme-Linked Immunosorbent AssayEstradiolEstrenesEstrogen Receptor ModulatorsEstrogensFemaleFuransGlucocorticoidsHormone AntagonistsHumansInsulin-Like Growth Factor Binding Protein 1Medroxyprogesterone AcetateMifepristoneNorpregnanesNorpregnenesProlactinReal-Time Polymerase Chain ReactionRNA, MessengerStromal CellsConceptsHuman endometrial stromal cellsCultured human endometrial stromal cellsInsulin-like growth factorEndometrial stromal cellsIGFBP-1RT/PCRTibolone administrationStromal cellsGrowth factorEffects of tiboloneProlactin expressionProtein-1 expressionEndometrial atrophyTibolone metabolitesWestern blot analysisEndometrial bleedingMedroxyprogesterone acetateProlactin levelsTiboloneSpecific ELISAChronic effectsProlactin outputProtein 1ELISA resultsBlot analysis
2014
Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells
Shapiro JP, Basar M, Kayisli UA, Guzeloglu-Kayisli O, Huang S, Suarez AA, Ozer HG, Schatz F, Lockwood CJ. Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells. Contraception 2014, 91: 253-260. PMID: 25529278, PMCID: PMC4395468, DOI: 10.1016/j.contraception.2014.11.005.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsUterine bleedingStanniocalcin-1Cultured human endometrial stromal cellsStromal cellsMicroarray analysisEndometrial biopsyTubal metaplasiaMRNA microarray analysisSide effectsLAPCComplex biological responseEtonogestrelHypoxia treatmentProgestinsHypoxiaPrimary culturesPotential mediatorsPotential targetProtein mediatorsBleedingExpression levelsMedium supernatantLong-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis
Guzeloglu-Kayisli O, Basar M, Shapiro J, Semerci N, Huang J, Schatz F, Lockwood C, Kayisli U. Long-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis. The Journal Of Clinical Endocrinology & Metabolism 2014, 99: e1957-e1966. PMID: 25029423, PMCID: PMC4184079, DOI: 10.1210/jc.2014-1770.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCaspase 3Cell ProliferationCells, CulturedContraceptive Agents, FemaleC-Reactive ProteinCulture Media, ConditionedCytochromes cEndometriumEndothelial CellsEstradiolFemaleHumansMedroxyprogesterone AcetateMicrovesselsNerve Tissue ProteinsParacrine CommunicationProgestinsReactive Oxygen SpeciesStromal CellsConceptsHuman endometrial endothelial cellsNeuronal pentraxin 1Abnormal uterine bleedingUterine bleedingMedroxyprogesterone acetateReactive oxygen species generationCultured human endometrial endothelial cellsOxygen species generationHuman endometrial stromal cellsUterine blood flowEndometrium of womenEndometrial endothelial cellsEndometrial stromal cellsProgesterone receptor proteinCaspase-3Elevated reactive oxygen species (ROS) generationHESC-CMsNovel paracrine mechanismEndothelial cell apoptosisSpecies generationFragile microvesselsCytosolic cytochrome c levelsParacrine mechanismsBlood flowLAPC
2006
Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells
Selam B, Kayisli UA, Akbas GE, Basar M, Arici A. Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells. Biology Of Reproduction 2006, 75: 203-209. PMID: 16687653, DOI: 10.1095/biolreprod.105.045716.Peer-Reviewed Original ResearchConceptsFASLG expressionEndometrial stromal cellsStromal cellsChemokine ligand 2Protein expressionSemiquantitative RT-PCR analysisRT-PCR analysisFAS ligand expressionHuman endometriumCellular growthWestern blot analysisRate of apoptosisCultured endometrial stromal cellsExtracellular matrixJurkat cellsIncreased apoptotic rateHuman endometrial stromal cellsApoptotic moleculesLevels of CCL2Blot analysisDynamic tissueNumerous hormonesApoptosisHuman endometrial cellsSame cells