2001
Ex vivo evaluation of PBMNCs collected with a new cell separator
Snyder E, O'Donnell L, Dengler T, Pomper G, Velleca M, Dincecco D, Baril L, Min K, Gudino M, Bender J. Ex vivo evaluation of PBMNCs collected with a new cell separator. Transfusion 2001, 41: 940-949. PMID: 11452164, DOI: 10.1046/j.1537-2995.2001.41070940.x.Peer-Reviewed Original ResearchMeSH KeywordsBlood DonorsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DivisionCell SeparationCytotoxicity Tests, ImmunologicEndothelium, VascularE-SelectinHematopoietic Stem Cell TransplantationHumansInfant, NewbornKiller Cells, NaturalLeukapheresisLeukocytes, MononuclearTime FactorsUmbilical VeinsConceptsDendritic cellsNK cellsCell separatorHealthy volunteer blood donorsMNC subsetsEndothelial cellsVolunteer blood donorsB cell subsetsCell surface markersAmicus SeparatorNK cytotoxicityBlood donorsSelectin expressionAppropriate cell surface markersCD8CD4Ex vivoSurface markersCD19CD3Study designProliferation assaysSelectin inductionGrowth factorFunctional studies
1997
Cytoskeletal rearrangement mediates human microvascular endothelial tight junction modulation by cytokines
Blum M, Toninelli E, Anderson J, Balda M, Zhou J, O'Donnell L, Pardi R, Bender J. Cytoskeletal rearrangement mediates human microvascular endothelial tight junction modulation by cytokines. American Journal Of Physiology 1997, 273: h286-h294. PMID: 9249502, DOI: 10.1152/ajpheart.1997.273.1.h286.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineActinsBucladesineCells, CulturedCytokinesCytoskeletonElectrophysiologyEndothelium, VascularHumansInfant, NewbornInterferon-gammaMaleMembrane PotentialsMembrane ProteinsMicrocirculationPhosphoproteinsSkinTight JunctionsTumor Necrosis Factor-alphaUmbilical VeinsZonula Occludens-1 ProteinConceptsTransmonolayer electrical resistanceSpecialized intercellular structuresTight junctionsF-actin cytoskeletonLarge vessel ECActin cytoskeletonEndothelial cell typesMicrovascular endothelial cellsCytoskeletal rearrangementsStriking fragmentationF-actinProtein distributionCytochalasin DCell typesIntercellular structuresBarrier regulationCytoskeletal alterationsConfocal microscopyMVEC monolayersZO-1Macromolecular fluxCentral roleEC permeabilityEndothelial cellsPrimary cultures
1991
Endothelial cell class II HLA expression induced by cytotoxic lymphocytes is regulated by genetically determined differences in CD11a/CD18.
Bender J, Tackett L, Pardi R. Endothelial cell class II HLA expression induced by cytotoxic lymphocytes is regulated by genetically determined differences in CD11a/CD18. Transplantation Proceedings 1991, 23: 99-101. PMID: 1671312.Peer-Reviewed Original Research
1990
T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells.
Bender J, Pardi R, Engleman E. T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 6949-6953. PMID: 2402485, PMCID: PMC54659, DOI: 10.1073/pnas.87.18.6949.Peer-Reviewed Original ResearchConceptsMicrovascular endothelial cellsEndothelial cellsNK cellsNatural killer cell targetsNatural killer cellsAllogeneic endothelial cellsAntigen-specific cytotoxicityNK cell linesCD3/T cell receptorLevel of adherenceEC linesHuman microvascular endothelial cellsT cell receptorOrgan allograftsGamma chain geneKiller cellsLymphoid cellsSpecific cytotoxicityCell targetsNegative cellsCell linesHuman erythroleukemia cellsSelective cytotoxicityTi moleculesCytotoxicity