2021
Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila
Gonzalez LE, Tang X, Lin H. Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila. Genetics 2021, 219: iyab091. PMID: 34142134, PMCID: PMC8757300, DOI: 10.1093/genetics/iyab091.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsGermline developmentPiwi knockdownPIWI proteinsEarly embryosPiwi/piRNA complexesPIWI/piRNA pathwayFemale progenyPrimordial germ cell developmentGermline sex determinationLoss of PiwiGermline stem cellsDrosophila early embryosGerm cell developmentGerm cell proliferationGonad coalescencePiRNA complexesPiRNA pathwayPiRNA poolTransposon suppressionZygotic genesEmbryonic germlineEmbryonic functionEarly embryogenesisPiwi
2016
Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins
Peng JC, Valouev A, Liu N, Lin H. Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins. Nature Genetics 2016, 48: 283-291. PMID: 26780607, PMCID: PMC4767590, DOI: 10.1038/ng.3486.Peer-Reviewed Original Research
2015
Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis
Gonzalez J, Qi H, Liu N, Lin H. Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis. Cell Reports 2015, 12: 150-161. PMID: 26119740, PMCID: PMC4497877, DOI: 10.1016/j.celrep.2015.06.004.Peer-Reviewed Original ResearchConceptsGermline stem cellsDrosophila testisSomatic cyst cellsPIWI-piRNA pathwayStem cell maintenanceCell-autonomous functionStem cellsGerm cell differentiationStem cell typesFasciclin 3GSC daughtersGermline functionSomatic rolePiwi expressionCyst cellsCell maintenanceEpigenetic mechanismsPiwiGenes keyNuclear localizationKey regulatorMolecular mechanismsCell differentiationGonadal developmentCell types
2014
Piwi Is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary
Ma X, Wang S, Do T, Song X, Inaba M, Nishimoto Y, Liu LP, Gao Y, Mao Y, Li H, McDowell W, Park J, Malanowski K, Peak A, Perera A, Li H, Gaudenz K, Haug J, Yamashita Y, Lin H, Ni JQ, Xie T. Piwi Is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. PLOS ONE 2014, 9: e90267. PMID: 24658126, PMCID: PMC3962343, DOI: 10.1371/journal.pone.0090267.Peer-Reviewed Original ResearchConceptsGerm cell differentiationDrosophila ovaryGermline stem cellsSomatic cellsCell differentiationGerm cellsPiRNA productionMultiple cell typesGSC maintenanceTransposable elementsGerm lineLineage developmentCell typesPiRNA pathway componentsGerm cell maintenanceCell lineage developmentUndifferentiated germ cellsCell-specific knockdownEscort cellsGSC establishmentGSC lossPiwi knockdownPiRNA pathwayGenome stabilityTE transcripts
2010
Identification of Piwil2-Like (PL2L) Proteins that Promote Tumorigenesis
Ye Y, Yin DT, Chen L, Zhou Q, Shen R, He G, Yan Q, Tong Z, Issekutz AC, Shapiro CL, Barsky SH, Lin H, Li JJ, Gao JX. Identification of Piwil2-Like (PL2L) Proteins that Promote Tumorigenesis. PLOS ONE 2010, 5: e13406. PMID: 20975993, PMCID: PMC2958115, DOI: 10.1371/journal.pone.0013406.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsBase SequenceCell Line, TumorCell Transformation, NeoplasticDNA PrimersFemaleHumansImmunohistochemistryMaleMiceMice, Inbred C57BLNeoplasm MetastasisNeoplasmsNF-kappa BPrecancerous ConditionsProteinsProto-Oncogene Proteins c-bcl-2Reverse Transcriptase Polymerase Chain ReactionRNA InterferenceRNA, MessengerSTAT3 Transcription FactorStem CellsTransplantation, HeterologousUp-RegulationConceptsGermline stem cellsStem cellsAGO gene familyCell cycle entryPrecancerous stem cellsTumor developmentTumor cell survivalBCL2 gene expressionGene familyCycle entryGene expressionTumor cellsNF-κBCell survivalAnticancer drug developmentPIWIL2S phaseGene exonsNovel insightsProteinMouse tumor cellsCancer developmentNovel monoclonal antibodyTranscriptsTypes of humanA Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal
Smulders-Srinivasan TK, Szakmary A, Lin H. A Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal. Genetics 2010, 186: 573-583. PMID: 20647505, PMCID: PMC2954472, DOI: 10.1534/genetics.110.119081.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsChromatinChromosomal Proteins, Non-HistoneDNA-Binding ProteinsDrosophilaDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHedgehog ProteinsMalePolycomb-Group ProteinsPolymerase Chain ReactionRepressor ProteinsRNA, Small InterferingRNA-Induced Silencing ComplexSignal TransductionStem Cell NicheStem CellsConceptsGermline stem cellsNiche cellsEpigenetic programmingStem Cell Self-RenewalEnhancer of PolycombTrithorax group genesPotential genetic interactionsCell Self-RenewalNovel epigenetic mechanismStem cellsDrosophila ovaryGSC divisionChromatin factorsNiche signalingChromatin proteinsGenetic interactionsPiwi expressionStem cell defectEpigenetic regulationPiwiEpigenetic mechanismsGroup genesRNA mechanismExpression of hedgehogSelf-Renewal
2009
The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster
Szakmary A, Reedy M, Qi H, Lin H. The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster. Journal Of Cell Biology 2009, 185: 613-627. PMID: 19433453, PMCID: PMC2711570, DOI: 10.1083/jcb.200903034.Peer-Reviewed Original ResearchConceptsYB proteinsNiche cellsYb bodiesElectron-dense spheresDrosophila melanogasterGermline stem cell maintenanceMale germline stem cellsGermline stem cellsStem cell maintenanceStem cellsC-terminal regionSomatic stem cellsN-terminal regionD. melanogasterNovel organellePiwi expressionGermline cellsDouble mutantCell maintenanceNovel lociSomatic cellsCytoplasmic spotsMelanogasterCytoplasmic structuresGermline loss
2008
Cell biology of stem cells: an enigma of asymmetry and self-renewal
Lin H. Cell biology of stem cells: an enigma of asymmetry and self-renewal. Journal Of Cell Biology 2008, 180: 257-260. PMID: 18227277, PMCID: PMC2213586, DOI: 10.1083/jcb.200712159.Peer-Reviewed Original ResearchConceptsCell biologyStem cellsIntrinsic cell polarityDrosophila melanogaster neuroblastsGermline stem cellsStem cell divisionStem cell systemCell cycle regulatorsSkin stem cellsNiche signalingCell polarityAsymmetric divisionDaughter cellsCell divisionCycle regulatorsStem cell researchBiologyCell researchCell systemCellsCrucial roleTherapeutic applicationsDivisionDiverse typesCentrosomes
2006
Sex-lethal is a target of Bruno-mediated translational repression in promoting the differentiation of stem cell progeny during Drosophila oogenesis
Wang Z, Lin H. Sex-lethal is a target of Bruno-mediated translational repression in promoting the differentiation of stem cell progeny during Drosophila oogenesis. Developmental Biology 2006, 302: 160-168. PMID: 17067567, PMCID: PMC1904479, DOI: 10.1016/j.ydbio.2006.09.016.Peer-Reviewed Original ResearchConceptsBruno response elementCystoblast differentiationTranslational repressionElectrophoresis mobility shift assaysGermline stem cellsPotential mRNA targetsMobility shift assaysStem cell progenySex-lethalDrosophila ovaryDrosophila oogenesisMutant phenotypeBioinformatics approachMRNA targetsShift assaysType RNACDNA constructsUntranslated regionCell progenyResponse elementStem cellsNovel targetDifferentiationRepressionMS11
2005
The Division of Drosophila Germline Stem Cells and Their Precursors Requires a Specific Cyclin
Wang Z, Lin H. The Division of Drosophila Germline Stem Cells and Their Precursors Requires a Specific Cyclin. Current Biology 2005, 15: 328-333. PMID: 15723793, DOI: 10.1016/j.cub.2005.02.016.Peer-Reviewed Original ResearchConceptsPrimordial germ cellsGSC divisionG2 cyclinsDrosophila germline stem cellsCyclin BGermline stem cellsCell-autonomous functionSpecific cell cycle regulatorsStem cell biologyStem cell cycleStem cellsCell cycle regulatorsSomatic lineagesFemale GSCsMutant defectsB cyclinsRedundant rolesSpecific cyclinsCell biologyDistinct functionsCycle regulatorsCell cycleGerm cellsCycBCyclin ARegulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation
Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Current Biology 2005, 15: 171-178. PMID: 15668175, DOI: 10.1016/j.cub.2005.01.005.Peer-Reviewed Original ResearchConceptsGermline stem cellsPiwi functionDrosophila ovarian germline stem cellsOvarian germline stem cellsStem Cell Self-RenewalFunction of PiwiDifferentiated daughter cellsCell Self-RenewalGerm cell divisionBam proteinCystoblast differentiationBam expressionMutant ovariesNiche signalsDouble mutantDaughter cellsDifferentiation genesPiwiCell divisionNiche cellsRegulatory relationshipsSelf-RenewalGerm cellsMutantsCystoblasts
2004
Germline stem cells in the Drosophila ovary descend from pole cells in the anterior region of the embryonic gonad
Asaoka M, Lin H. Germline stem cells in the Drosophila ovary descend from pole cells in the anterior region of the embryonic gonad. Development 2004, 131: 5079-5089. PMID: 15459101, DOI: 10.1242/dev.01391.Peer-Reviewed Original ResearchConceptsGermline stem cellsPrimordial germ cellsEmbryonic gonadsOnset of oogenesisSomatic cellsNiche cellsGSC fatePole cellsStem cellsSpecific somatic cellsStem cell biologyTissue stem cellsDE-cadherinLarval gonadsPGC proliferationCell biologyLate larvalAdult ovariesOogenesisGerm cellsDrosophilaGonadsNanos Maintains Germline Stem Cell Self-Renewal by Preventing Differentiation
Wang Z, Lin H. Nanos Maintains Germline Stem Cell Self-Renewal by Preventing Differentiation. Science 2004, 303: 2016-2019. PMID: 14976263, DOI: 10.1126/science.1093983.Peer-Reviewed Original ResearchConceptsGermline stem cellsPrimordial germ cellsGermline cystsStem Cell Self-RenewalTranslational repressor NanosCell Self-RenewalStem cellsDrosophila ovaryGene regulationExtrinsic signalingPrecocious entrySelf-RenewalGerm cellsCell typesDifferentiation factorCellsCystoblastsOogenesisSignalingDifferentiationRegulationOvariesTranslation
2003
Screens for piwi Suppressors in Drosophila Identify Dosage-Dependent Regulators of Germline Stem Cell Division
Smulders-Srinivasan TK, Lin H. Screens for piwi Suppressors in Drosophila Identify Dosage-Dependent Regulators of Germline Stem Cell Division. Genetics 2003, 165: 1971-1991. PMID: 14704180, PMCID: PMC1462913, DOI: 10.1093/genetics/165.4.1971.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedArgonaute ProteinsCell DivisionChromosomesDrosophilaDrosophila ProteinsFemaleGene DosageGene Expression Regulation, DevelopmentalGenes, LethalGerm CellsInfertility, MaleMaleProteinsRNA-Induced Silencing ComplexStem CellsSuppression, GeneticTranscription, GeneticZygoteConceptsStem cell divisionGermline stem cell divisionGenes/sequencesCell divisionFemale-specific lethalityDosage-dependent regulatorGermline stem cellsStem cell maintenanceDrosophila third chromosomeFamily of genesDosage-sensitive mannerPiwi mutantsZygotic functionMutant backgroundPiwi genesThird chromosomeMale germlineMale sterilitySuppressor mutationsSuch genesCell maintenanceGenetic regulationPlant kingdomTranscription factorsTranscriptional level
2002
The stem-cell niche theory: lessons from flies
Lin H. The stem-cell niche theory: lessons from flies. Nature Reviews Genetics 2002, 3: 931-940. PMID: 12459723, DOI: 10.1038/nrg952.Peer-Reviewed Original ResearchConceptsStem cell nicheSomatic stem cellsStem cellsTesticular stem cell nicheGermline stem cellsTerminal filament cellsSelf-renewing divisionsOogenic functionEpidermal growth factor receptorUnpaired ligandAdherens junctionsMammalian systemsTesticular nicheOvarian nicheGrowth factor receptorHub cellsPathway functionNiche theoryFilament cellsGermlineCell adhesionNicheFactor receptorPathwayFlies