2024
Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Baldini M, Zhang C, Yu J. Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients. Methods In Molecular Biology 2024, 2782: 113-122. PMID: 38622396, DOI: 10.1007/978-1-0716-3754-8_8.Peer-Reviewed Original ResearchConceptsMitochondria-associated membranesEndoplasmic reticulumIntercellular adhesion molecule-1Vascular cell adhesion molecule-1Adhesion molecule-1Toll-like receptorsEndothelial cellsPercoll step gradientMolecule-1Pattern recognition receptorsLipid traffickingER stressCell adhesion molecule-1Innate immune cellsLevels of pattern recognition receptorsStep gradientSubcellular fractionsExpressing adhesion moleculesExpression levels of pattern recognition receptorsRecognition receptorsImmune cellsCalcium signalingExpression levelsEndothelial functionE-selectinSaponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway
Liu L, Qiu L, Xue J, Zhong C, Qin M, Zhang Y, Xu C, Xie Y, Yu J. Saponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway. Molecules 2024, 29: 1239. PMID: 38542876, PMCID: PMC10976117, DOI: 10.3390/molecules29061239.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Acute lung injuryEndothelial inflammationLung injuryNF-kBAugmented expression of adhesion moleculesLung injury in vivoAcute lung infectionAcute lung injury in vivoExpression of adhesion moleculesSecretion of pro-inflammatory cytokinesVascular cell adhesion molecule-1 expressionTumor necrosis factor-aCell adhesion molecule-1Septic acute lung injuryNF-kB p65 phosphorylationAdhesion molecule-1Coronary artery diseaseInjury in vivoPro-inflammatory cytokinesHuman umbilical vein endothelial cellsInflammatory mediators lipopolysaccharideExpression of pro-inflammatory factorsUmbilical vein endothelial cellsPro-inflammatory factors
2023
Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization
Yang X, Wu M, Yan X, Zhang C, Luo Y, Yu J. Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization. Molecules 2023, 28: 3682. PMID: 37175092, PMCID: PMC10179893, DOI: 10.3390/molecules28093682.Peer-Reviewed Original ResearchConceptsBone marrow-derived macrophagesAnti-tumor effectsM2 macrophage polarizationAnti-tumor activityB16-F0 cellsPolarization of M2 macrophagesTumor progressionMacrophage polarizationExpression of M2 marker genesIn vivoM2 macrophagesConditioned mediumLung metastasis of melanomaIn vitroPhosphorylated STAT6Bone marrow suppressionMRNA levelsMetastasis of melanomaSuppress tumor progressionDown-regulationMarrow-derived macrophagesMetastasis of tumorsSTAT6 signaling pathwayMarrow suppressionM2 marker genes
2020
Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis
Fu H, Sun Y, Shao Y, Saredy J, Cueto R, Liu L, Drummer C, Johnson C, Xu K, Lu Y, Li X, Meng S, Xue E, Tan J, Jhala N, Yu D, Zhou Y, Bayless K, Yu J, Rogers T, Hu W, Snyder N, Sun J, Qin X, Jiang X, Wang H, Yang X. Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis. Frontiers In Immunology 2020, 11: 595813. PMID: 33154757, PMCID: PMC7591706, DOI: 10.3389/fimmu.2020.595813.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesIL-35Ischemia-induced angiogenesisHindlimb ischemiaOmics data analysisApoE-/- controlsLoss-of-functionEndothelial cellsExtracellular matrix remodeling proteinsRegenerative angiogenesisExtracellular matrix proteinsGain-of-functionRNA-seqExtracellular matrix re-organizationIL-6STMatrix remodeling proteinsTranscriptome analysisLate phaseTube formation <i>IL-12RB2Interleukin (ILTranscriptome analysis of endothelial cellsIL-12 familyMatrix proteinsAnalysis of endothelial cellsAn allosteric site on MKP5 reveals a strategy for small-molecule inhibition
Gannam Z, Min K, Shillingford SR, Zhang L, Herrington J, Abriola L, Gareiss PC, Pantouris G, Tzouvelekis A, Kaminski N, Zhang X, Yu J, Jamali H, Ellman JA, Lolis E, Anderson KS, Bennett AM. An allosteric site on MKP5 reveals a strategy for small-molecule inhibition. Science Signaling 2020, 13 PMID: 32843541, PMCID: PMC7569488, DOI: 10.1126/scisignal.aba3043.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric SiteAmino Acid SequenceAnimalsCell DifferentiationCell LineDual-Specificity PhosphatasesEnzyme InhibitorsFemaleHigh-Throughput Screening AssaysHumansKineticsMiceMice, KnockoutMitogen-Activated Protein Kinase PhosphatasesMyoblastsProtein BindingSequence Homology, Amino AcidSignal TransductionSmall Molecule LibrariesConceptsDystrophic muscle diseaseMitogen-activated protein kinaseMuscle diseaseTGF-β1Promising therapeutic targetP38 mitogen-activated protein kinaseTherapeutic strategiesTherapeutic targetSmall molecule inhibitionSmad2 phosphorylationDiseasePotential targetSmall-molecule screenInhibitorsTreatmentInhibition