2024
Saponins from Allii Macrostemonis Bulbus attenuate atherosclerosis by inhibiting macrophage foam cell formation and inflammation
Zhao S, Guo H, Qiu L, Zhong C, Xue J, Qin M, Zhang Y, Xu C, Xie Y, Yu J. Saponins from Allii Macrostemonis Bulbus attenuate atherosclerosis by inhibiting macrophage foam cell formation and inflammation. Scientific Reports 2024, 14: 12917. PMID: 38839811, PMCID: PMC11153636, DOI: 10.1038/s41598-024-61209-w.Peer-Reviewed Original ResearchConceptsBone marrow-derived macrophagesMarrow-derived macrophagesFoam cell formationMacrophage foam cell formationLipid-loweringCell formationApolipoprotein E-deficientHigh-fat diet feedingInhibit macrophage foam cell formationClinical management of atherosclerosisAtherosclerosis plaque formationAnti-atherosclerotic effectsAnti-inflammatory propertiesManagement of atherosclerosisClinical managementAttenuate inflammationLPS-induced inflammatory responseAllii Macrostemonis BulbusCD36 expressionTherapeutic strategiesIn vitro experimentsInflammatory responseAnti-coagulationE deficiencyHigh-fatSaponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway
Liu L, Qiu L, Xue J, Zhong C, Qin M, Zhang Y, Xu C, Xie Y, Yu J. Saponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway. Molecules 2024, 29: 1239. PMID: 38542876, PMCID: PMC10976117, DOI: 10.3390/molecules29061239.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Acute lung injuryEndothelial inflammationLung injuryNF-kBAugmented expression of adhesion moleculesLung injury in vivoAcute lung infectionAcute lung injury in vivoExpression of adhesion moleculesSecretion of pro-inflammatory cytokinesVascular cell adhesion molecule-1 expressionTumor necrosis factor-aCell adhesion molecule-1Septic acute lung injuryNF-kB p65 phosphorylationAdhesion molecule-1Coronary artery diseaseInjury in vivoPro-inflammatory cytokinesHuman umbilical vein endothelial cellsInflammatory mediators lipopolysaccharideExpression of pro-inflammatory factorsUmbilical vein endothelial cellsPro-inflammatory factors
2023
Tiliroside Attenuates NLRP3 Inflammasome Activation in Macrophages and Protects against Acute Lung Injury in Mice
Zhong C, Yang J, Deng K, Lang X, Zhang J, Li M, Qiu L, Zhong G, Yu J. Tiliroside Attenuates NLRP3 Inflammasome Activation in Macrophages and Protects against Acute Lung Injury in Mice. Molecules 2023, 28: 7527. PMID: 38005247, PMCID: PMC10673355, DOI: 10.3390/molecules28227527.Peer-Reviewed Original ResearchConceptsAMP-activated protein kinaseNLRP3 inflammasome activationInflammasome activationReduction of mitochondrial reactive oxygen speciesMitochondrial reactive oxygen speciesMultiprotein signaling complexesMitochondrial membrane potentialImprovement of mitochondrial membrane potentialNLRP3-related diseasesAmeliorating mitochondrial damageSignaling complexProtein kinaseReactive oxygen speciesRegulating NLRP3 inflammasome activationNLRP3 inflammasomeMitochondrial damageInnate immunityDysregulated NLRP3 inflammasome activationAttenuated NLRP3 inflammasome activationNOD-like receptor family pyrin domainMembrane potentialOxygen speciesPyrin domainFamily pyrin domainDietary plantsTiliroside Protects against Lipopolysaccharide-Induced Acute Kidney Injury via Intrarenal Renin–Angiotensin System in Mice
Yi X, Xu C, Yang J, Zhong C, Yang H, Tang L, Song S, Yu J. Tiliroside Protects against Lipopolysaccharide-Induced Acute Kidney Injury via Intrarenal Renin–Angiotensin System in Mice. International Journal Of Molecular Sciences 2023, 24: 15556. PMID: 37958538, PMCID: PMC10648967, DOI: 10.3390/ijms242115556.Peer-Reviewed Original ResearchConceptsAcute kidney injuryLPS-induced acute kidney injuryIntrarenal renin-angiotensin systemRenin-angiotensin systemTubular cell apoptosisAng IIKidney injuryPlasma Ang IIKidney injury markersAmeliorated renal dysfunctionCell apoptosisMale C57BL/6 miceSeveral inflammatory diseasesLPS-injected miceOxidative stressLPS-induced AKI miceLipopolysaccharide (LPS)-induced acute kidney injuryInhibiting oxidative stressRenal dysfunctionRenal expressionRenoprotective actionC57BL/6 miceRenoprotective effectsFibrosis markersInjury markersDifferential regulation of autophagy on urine-concentrating capability through modulating the renal AQP2 expression and renin-angiotensin system in mice
Xu C, Yi X, Tang L, Wang H, Chu S, Yu J. Differential regulation of autophagy on urine-concentrating capability through modulating the renal AQP2 expression and renin-angiotensin system in mice. American Journal Of Physiology. Renal Physiology 2023, 325: f503-f518. PMID: 37589054, DOI: 10.1152/ajprenal.00018.2023.Peer-Reviewed Original ResearchConceptsUrine-concentrating capabilityAquaporin-2Renal cortexAngiotensin-converting enzymeAng IIDownregulation of aquaporin-2Mechanism of autophagyEffect of autophagyRenal pathophysiologyWater deprivationRenal AQP2 expressionRenal aquaporin-2Function of autophagyRegulation of autophagyUrine-concentrating abilityRenin-angiotensin systemKidneys of miceAQP2 expressionAldosterone levelsWD miceAngiotensin IICapability of miceRapa administrationRenal medullaMiceEffects of maternal hypothyroidism on postnatal cardiomyocyte proliferation and cardiac disease responses of the progeny
Li Y, Johnson J, Yang Y, Yu D, Kubo H, Berretta R, Wang T, Zhang X, Foster M, Yu J, Tilley D, Houser S, Chen X. Effects of maternal hypothyroidism on postnatal cardiomyocyte proliferation and cardiac disease responses of the progeny. AJP Heart And Circulatory Physiology 2023, 325: h702-h719. PMID: 37539452, PMCID: PMC10659327, DOI: 10.1152/ajpheart.00320.2023.Peer-Reviewed Original ResearchConceptsTransverse aortic constrictionMaternal hypothyroidismCM proliferationIn vitro studiesPressure overloadAdult heartDisease responseEffects of maternal hypothyroidismPathological remodelingHeart weightCM numbersBone morphogenetic protein 10Postnatal cardiomyocyte proliferationIn vivoBody weightProgeny of mothersCardiac pathological remodelingCardiac myocytesMyocyte proliferationPostnatal dayAortic constrictionPostnatal cardiomyocytesProtein 10Synthetic thyroxinePostnatal developmentPulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization
Yang X, Wu M, Yan X, Zhang C, Luo Y, Yu J. Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization. Molecules 2023, 28: 3682. PMID: 37175092, PMCID: PMC10179893, DOI: 10.3390/molecules28093682.Peer-Reviewed Original ResearchConceptsBone marrow-derived macrophagesAnti-tumor effectsM2 macrophage polarizationAnti-tumor activityB16-F0 cellsPolarization of M2 macrophagesTumor progressionMacrophage polarizationExpression of M2 marker genesIn vivoM2 macrophagesConditioned mediumLung metastasis of melanomaIn vitroPhosphorylated STAT6Bone marrow suppressionMRNA levelsMetastasis of melanomaSuppress tumor progressionDown-regulationMarrow-derived macrophagesMetastasis of tumorsSTAT6 signaling pathwayMarrow suppressionM2 marker genesCombining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype
Li Y, Kubo H, Yu D, Yang Y, Johnson J, Eaton D, Berretta R, Foster M, McKinsey T, Yu J, Elrod J, Chen X, Houser S. Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype. AJP Heart And Circulatory Physiology 2023, 324: h443-h460. PMID: 36763506, PMCID: PMC9988529, DOI: 10.1152/ajpheart.00594.2022.Peer-Reviewed Original ResearchConceptsSuberoylanilide hydroxamic acidHFpEF phenotypeHigh-fat dietAtrial natriuretic peptideEjection fractionHeart failureCM hypertrophyMouse modelL-NAMEMyocardial fibrosisTesting of novel therapeutic strategiesTherapeutic strategiesSuberoylanilide hydroxamic acid treatmentPathological stressorsHistone deacetylasesIncreased atrial natriuretic peptideFeatures of HFpEFL-NAME treatmentDiastolic filling pressureWild-typeNovel therapeutic strategiesMonths of high-fat dietInvasive hemodynamicsDiastolic dysfunctionWT mice
2022
Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue
Leigh T, Kawai T, Preston K, Kelemen S, Okune R, St Paul A, Corbett C, Peluzzo A, Yu J, Scalia R, Autieri M. Deletion of LDLRAP1 Induces Atherosclerotic Plaque Formation, Insulin Resistance, and Dysregulated Insulin Response in Adipose Tissue. American Journal Of Pathology 2022, 192: 1092-1108. PMID: 35460615, PMCID: PMC9253916, DOI: 10.1016/j.ajpath.2022.03.014.Peer-Reviewed Original ResearchConceptsLow density lipoprotein receptor adaptor protein 1Visceral adipose tissueInsulin resistanceAdipose tissueAdaptor protein 1Metabolic regulatory rolesHigh-fat Western dietComorbidities of obesityWild-type controlsGene expression signaturesIncreased plasma cholesterolReduced glucose uptakeIncreased CD36 expressionIncreased plaque burdenPreclinical modelsLipid storageChow dietRegulatory roleVascular inflammationCD36 expressionWestern dietAtherosclerotic plaque formationMetabolic profileAkt phosphorylationEnergy homeostasis
2020
An allosteric site on MKP5 reveals a strategy for small-molecule inhibition
Gannam Z, Min K, Shillingford SR, Zhang L, Herrington J, Abriola L, Gareiss PC, Pantouris G, Tzouvelekis A, Kaminski N, Zhang X, Yu J, Jamali H, Ellman JA, Lolis E, Anderson KS, Bennett AM. An allosteric site on MKP5 reveals a strategy for small-molecule inhibition. Science Signaling 2020, 13 PMID: 32843541, PMCID: PMC7569488, DOI: 10.1126/scisignal.aba3043.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric SiteAmino Acid SequenceAnimalsCell DifferentiationCell LineDual-Specificity PhosphatasesEnzyme InhibitorsFemaleHigh-Throughput Screening AssaysHumansKineticsMiceMice, KnockoutMitogen-Activated Protein Kinase PhosphatasesMyoblastsProtein BindingSequence Homology, Amino AcidSignal TransductionSmall Molecule LibrariesConceptsDystrophic muscle diseaseMitogen-activated protein kinaseMuscle diseaseTGF-β1Promising therapeutic targetP38 mitogen-activated protein kinaseTherapeutic strategiesTherapeutic targetSmall molecule inhibitionSmad2 phosphorylationDiseasePotential targetSmall-molecule screenInhibitorsTreatmentInhibition