Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Baldini M, Zhang C, Yu J. Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients. Methods In Molecular Biology 2024, 2782: 113-122. PMID: 38622396, DOI: 10.1007/978-1-0716-3754-8_8.Peer-Reviewed Original ResearchConceptsMitochondria-associated membranesEndoplasmic reticulumIntercellular adhesion molecule-1Vascular cell adhesion molecule-1Adhesion molecule-1Toll-like receptorsEndothelial cellsPercoll step gradientMolecule-1Pattern recognition receptorsLipid traffickingER stressCell adhesion molecule-1Innate immune cellsLevels of pattern recognition receptorsStep gradientSubcellular fractionsExpressing adhesion moleculesExpression levels of pattern recognition receptorsRecognition receptorsImmune cellsCalcium signalingExpression levelsEndothelial functionE-selectinEdgeworthia gardneri (Wall.) Meisn. Ethanolic Extract Attenuates Endothelial Activation and Alleviates Cardiac Ischemia-Reperfusion Injury
Lang X, Zhong C, Su L, Qin M, Xie Y, Shan D, Cui Y, Shi M, Li M, Quan H, Qiu L, Zhong G, Yu J. Edgeworthia gardneri (Wall.) Meisn. Ethanolic Extract Attenuates Endothelial Activation and Alleviates Cardiac Ischemia-Reperfusion Injury. Molecules 2024, 29: 1068. PMID: 38474581, PMCID: PMC10935089, DOI: 10.3390/molecules29051068.Peer-Reviewed Original ResearchConceptsExtracellular regulated protein kinasesEndothelium activationMitogen-activated protein kinaseI/R injuryEndothelial cellsCardiac ischemia-reperfusion injuryIschemia-reperfusion (I/RAdhesion to endothelial cellsIschemia-reperfusion injuryExpression of pro-inflammatory mediatorsNuclear factor kappa BCardiac I/R injuryPro-inflammatory mediatorsIn vivo findingsP38 mitogen-activated protein kinaseFactor kappa BPro-inflammatory activityI/R-induced inflammationMyocardial I/R injuryEndothelial activationCardiac injuryC-Jun <i>N</i>-terminal kinase (JTherapeutic efficacyFirm adhesion to endothelial cellsProtein kinase