Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Baldini M, Zhang C, Yu J. Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients. Methods In Molecular Biology 2024, 2782: 113-122. PMID: 38622396, DOI: 10.1007/978-1-0716-3754-8_8.Peer-Reviewed Original ResearchConceptsMitochondria-associated membranesEndoplasmic reticulumIntercellular adhesion molecule-1Vascular cell adhesion molecule-1Adhesion molecule-1Toll-like receptorsEndothelial cellsPercoll step gradientMolecule-1Pattern recognition receptorsLipid traffickingER stressCell adhesion molecule-1Innate immune cellsLevels of pattern recognition receptorsStep gradientSubcellular fractionsExpressing adhesion moleculesExpression levels of pattern recognition receptorsRecognition receptorsImmune cellsCalcium signalingExpression levelsEndothelial functionE-selectin