Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Baldini M, Zhang C, Yu J. Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients. Methods In Molecular Biology 2024, 2782: 113-122. PMID: 38622396, DOI: 10.1007/978-1-0716-3754-8_8.Peer-Reviewed Original ResearchConceptsMitochondria-associated membranesEndoplasmic reticulumIntercellular adhesion molecule-1Vascular cell adhesion molecule-1Adhesion molecule-1Toll-like receptorsEndothelial cellsPercoll step gradientMolecule-1Pattern recognition receptorsLipid traffickingER stressCell adhesion molecule-1Innate immune cellsLevels of pattern recognition receptorsStep gradientSubcellular fractionsExpressing adhesion moleculesExpression levels of pattern recognition receptorsRecognition receptorsImmune cellsCalcium signalingExpression levelsEndothelial functionE-selectinSaponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway
Liu L, Qiu L, Xue J, Zhong C, Qin M, Zhang Y, Xu C, Xie Y, Yu J. Saponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway. Molecules 2024, 29: 1239. PMID: 38542876, PMCID: PMC10976117, DOI: 10.3390/molecules29061239.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Acute lung injuryEndothelial inflammationLung injuryNF-kBAugmented expression of adhesion moleculesLung injury in vivoAcute lung infectionAcute lung injury in vivoExpression of adhesion moleculesSecretion of pro-inflammatory cytokinesVascular cell adhesion molecule-1 expressionTumor necrosis factor-aCell adhesion molecule-1Septic acute lung injuryNF-kB p65 phosphorylationAdhesion molecule-1Coronary artery diseaseInjury in vivoPro-inflammatory cytokinesHuman umbilical vein endothelial cellsInflammatory mediators lipopolysaccharideExpression of pro-inflammatory factorsUmbilical vein endothelial cellsPro-inflammatory factors