Ho Lab Photos
2023/11/16 Congrats to Michelle and Sam who won the CROI New Investigator Award!
Announcement of Michelle and Sam winning the CROI New Investigator award on the CROI website.
2023/11/2 Single-cell epigenetic, transcriptional, and protein profiling of latent and active HIV-1 reservoir revealed that IKZF3 promotes HIV-1 persistence” at Immunity by Yulong.
Single-cell multiomic DOGMA-seq captures in vivo states of HIV-1 DNA+ and HIV-1 RNA+ cells. HIV-1 latently infected cells are distinct at the DNA, RNA, and protein levels. HIV-1-infected cells have four distinct cell states: IRF, cytotoxic, AP-1, and cell death. We found that IKZF3 (Aiolos) promotes the proliferation of HIV-1-infected cells
10/12/2023 Ho Lab at the NIH Strategies for an HIV Cure Meeting
Our lab visited NIH for the Strategies for an HIV Cure Meeting. Ya-Chi gave a talk on behalf of BEAT-HIV Collaboratory. Yulong and Timmy each presented a poster. Kristen presented a poster as a BEAT-HIV young investigator awardee.
2023/8/3 “Single-cell multiomic understanding of HIV-1 reservoir at epigenetic, transcriptional, and protein levels”
Current Opinion HIV and AIDS by Michelle and Yulong
Understanding the heterogenous and rare HIV reservoir requires single-cell multiomics. What are ECCITEseq, FINDseq, PhePseq, ASAPseq, DOGMAseq? Why and how? Principles, method and bioinformatic guide reviewed by our postdocs Michelle Wong and Yulong Wei.
2023/7/25 New Approaches to measuring HIV reservoir, Plenary at 2023 IAS
Ya-Chi gave a plenary talk at the International AIDS Society Annual Meeting, Brisbane, Australia.
2023/7/14 Lab Retreat – HIV life cycle
Learning HIV life cycle by recapitulating Dr. David Goodsell’s beautiful and evidence-based illustrations in our lab retreat and discovering impressive talent of our lab members!
2023/6/12 “Integration site-dependent HIV-1 promoter activity shapes host chromatin interaction”
at Genome Research by Jack
Using DOGMAseq (single-cell RNAseq and ATACseq), HIV-1 4C, HiC, HiChIP, CRISPRa, CRISPRi, we found that HIV-1 promoter activity increased host chromatin accessibility, increased HIV-1-host chromatin interactions in an integration site–dependent manner, within the existing chromatin boundaries. This manuscript recapitulated Jack's Uta von Schwedler Prize talk at CSHL Retro 2022. Congrats to Jack, the first and corresponding author of this manuscript!
https://genome.cshlp.org/content/early/2023/06/09/gr.277698.123
2023/5/8 Ho Lab Networking hour
Spatial transcriptomics by hand-made waffles, ice cream, and sprinkles.
2023/5/2 Ho Lab at the REACH Martin Delaney Collaboratory Annual Meeting, Rockefeller University
The lab enjoyed stimulating talks with amazing scientists at the REACH Martin Delaney Collaboratory Annual Meeting.
2023/2/5
Ya-Chi is elected as a member of the American Society for Clinical Investigation
2023/2/22 Kristen presented “Spatial mapping of SIV-infected rhesus macaque lymph node environment during rebound”
As a New Investigator Awardee at CROI 2023, Seattle.
2023/2/1 Yulong gave a late-breaker talk
“The single-cell epigenetic, transcriptional, and protein states of HIV-infected cells” at CROI 2023, Seattle.
2022/10/31 Congratulations to Dr. Jack Collora for an exceptional PhD journey!
We choose to do it not because it's easy, but because it's hard. Jack pioneered single-cell multiomics in HIV and discovered cytotoxic CD4 as a major reservoir, with exceptional curiosity, altruism, excellence, and resilience. Jack graduated in 5.5 years as a 1st author of Immunity, a co-first author of Journal of Virology, a first and corresponding author of Genome Research, and a Uta von Schwedler Prize awardee. Thanks to the committee & congrats *Dr.* Collora!
2022/6/22 “Inhibition of a Chromatin and Transcription Modulator, SLTM, Increases HIV-1 Reactivation Identified by a CRISPR Inhibition Screen”
at Journal of Virology, by Savannah, Jack, and Rache
Our CRISPRi screen found HIV silencing factors: chromatin modulators & nuclear exosome decay NEXT (ZCCHC8 & RBM7) and PAXT (ZFC3H1). SLTM knockdown in clinical samples reduces the frequency of HIV+ cells.
2022/6/7 Ho Lab retreat – Glass blowing
Mind-blowing lab retreat by glass blowing! Now we have single-particle virion made of glass (idea by undergrad Sam), fantastic beakers for maxiprep, and unique colors such as mPumpkin.
2022/5/25 Jack received Uta von Schwedler Prize, CSHL Retroviruses
Jack gave a talk at the Cold Spring Harbor Laboratory Retroviruses meeting “The HIV-host chromatin interaction landscape” as the Uta von Schwedler Prize awardee and a poster, featured as the back cover of the meeting program book.
2022/5/13 Ho Lab Networking Hour
High-resolution, high-dimensional, multi-omic understanding of individual lab members by hosting an in-person happy hour!
2022/5/11 Visiting Boston!
Visited Sizun Jiang lab at CVVR/Beth Israel and Jonathan Li, Lichterfeld, Tsibris, Kurizkes labs at BWH for very stimulating discussions!
2022/5/5 Ho Lab visiting REACH Martin Delaney HIV Cure Collaboratory annual meeting
Thanks to an exciting program and collaboration led by Drs. Brad Jones and Marina Caskey!
2022/4/18 Yulong received AIDS 2022 Scholarship
For the IAS annual meeting at Montreal, Canada.
2022/4/8 Ya-Chi gave a Keynote Dr. Thomas A. and Joyce E. Pearson Endowed lecture
At the Medical Scientist Research Symposium at University of Rochester.
2022/3/22 “Single-cell multiomics reveals persistence of HIV-1 in expanded cytotoxic T cell clones”
at Immunity by Jack
Jack pioneered single-cell multiomic profiling of HIV-infected cells at their native states without ex vivo activation, for the first time in the field, and revealed that HIV persists by hiding in proliferative cytotoxic CD4+ T cell clones driven by antigen & TNF. Granzyme B inhibitor Serpin B9 may shield HIV-infected CD4+ CTLs from CD8+ CTL killing.
https://pubmed.ncbi.nlm.nih.gov/35320704/
2022/3/14 Yulong received the Natural Sciences and Engineering Research Council of Canada (NSERC) postdoctoral fellowship
Congrats to Yulong for receiving the prestigious award!
2022/2/14 Jack and Yulong received CROI New Investigator Scholarshop!
CROI 2022: Jack, Yulong, and Rachel are presenting 1 talk and 3 posters.
2022/1/28 Yulong received AIDS Cancer Specimen Repository (ACSR) Young Investigator Pilot Award!
If you torture the data long enough, it will confess," a bioinformatician who excelled at single-cell wet-lab methods now works on impact of HIV on epigenetic regulations of tumor immunity.
2021/12/10 Lab lunch - celebrating a fun year with amazing folks!
2021/12/10 Ho Lab Secret Santa
-Yulong (bioinformatician): "If you torture the data hard enough, it will confess." - from Afeez
-Amare (finding HIV): Where's Waldo book depicts HIV reservoir: if you can't find it, it doesn't mean it doesn't exist! - from Jack
-Kristen got a blanket that is in the same pattern as her spatial transcriptomics device DBiTseq. When she puts it on, she looks like a tissue under a spatial transcriptomics device. - from Jimmy
-Jack needs to glue diamonds pixel by pixel on his UMAP plot on a Seurat painting. - from Ya-Chi
-Rachel got a med school survival kit from Amare and Sam got a undergrad survival kit from Rachel.
2021 Ho Lab Annual Retreat - Workshop for Innovative Technologies
We spent an afternoon working on using innovative technologies (such as glass fusion) and creativity for scientific projects.
2020/12/11 Ho Lab Secret Santa under COVID-19
2020/9/4 Ho Lab HIV Trivia!
2019/12/13 Ho Lab Secret Santa: You never know how much your lab mates know and care about you (and how creative they can be)!
2019/12/6 Jack won the Ugly Sweater Contest at the Yale Micro Annual Gathering!
2019/9/13 Ho Lab presented 6 posters at the Yale Micro Retreat - and Runxia won the Best Poster Award!
Samana leaving for medical school!
2019 BEAT-HIV Annual Meeting, Wistar Institute, Philadelphia
Inspired by spectacular talks and exciting discussions with HIV basic scientists, clinicians and the community board. This is how the new generation of HIV scientists grow up!
2019 Ho Lab Annual Retreat - unleash creativity!
Left to right, top to bottom:
Ya-Chi: "We came, we sequence, we cure."
Savannah: "HIV we are coming for you!"
Jack: "It's just a side project." (He has many projects.)
Ryan: "Grow your ideas!" (He holds a plant grower.)
Runxia: "Professional FISHer!" (She does RNA FISH everyday.)
Kristen: "Refuse to fear - instead work to understand"
Samana: "Rise and Pipette"(She has 6 96-well plates to work on next week)
Jimmy: "We belong"
(Jack has many many projects)
(Samana is working on many 96 well plates)
(Ryan is holding a plant grower)
(Kristen is figuring out very challenging projects.)
(Savannah will continue to work on all aspects of HIV research.)
The Ho lab attending the NIH Strategies for an HIV Cure Meeting, 2018
PhD student Jack presented a poster at the NIH Strategies for an HIV Cure Meeting under BEAT-HIV Travel Award.
Postdoc Runxia presented a poster at the NIH Strategies for an HIV Cure Meeting under BEAT-HIV Travel Award.
Postdoc Jimmy presented a poster at the NIH Strategies for an HIV Cure Meeting under BEAT-HIV Travel Award.
2018 NIH Strategies for an HIV Cure Meeting
2018 AIDS (International AIDS Society Annual Meeting), Amsterdam
2018 Yale HIV Symposium
BEAT - Martin Delaney HIV Collaboratory Annual Meeting
http://beat-hiv.org/
2018 Cold Spring Harbor Meetings on Retroviruses
2018 Cold Spring Harbor Laboratory Meetings on Retroviruses - Ya-Chi recording TWiV (This Week in Virology) for Andy Kaplan Prize
- Topics discussed: CSHL meetings, HIV-1 RNA SortSeq, career advice for young scientists
2018 Cold Spring Harbor Meetings on Retroviruses
2018 Annual Ho Lab Retreat
2018/5/18 Ho Lab Retreat agenda:
10 am Chalk talks from all lab members
1 pm Webinar with collaborators
3 pm Enhance collaborations and explore intellectual potentials at Escape Room