Ongoing Projects

Memory B cells that differentiate into antigen presenting and antibody-producing cells are important in natural and vaccine-mediated protection and are targets of B cell-directed therapies for malignancy and autoimmune disease. Thus, there is great interest in elucidating their unique biological properties in order to understand how they form, function and modulate recall responses. However, progress in elucidating these properties has been limited, particularly in mice, by the lack of adequate markers to distinguish memory cells. Our group has had a long-standing interest in this area and has made a number of contributions.

Using mouse systems developed in our laboratory that overcome significant barriers to the study of B cell memory, we compared gene expression between memory B cells and their naïve precursors using Affymetrix microarrays. We confirmed the differential expression of several conceptually important families at the mRNA and protein level. These initial findings were the foundation of several research projects. Some key findings and areas of ongoing research include:

• Memory B cells are wired to signal differently than their naïve precursors.
• There are subsets of murine memory B cells that form a spectrum from more naïve-like to more-memory-like.
• Specific pathways regulate self-renewal and differentiation to antibody forming cells.
• Specific pathways regulate the quality of the T-dependant B cell immune response.