Recent Publications

• A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs.Brown C, Ghosh S, McAllister R, Kumar M, Walker G, Sun E, Aman T, Panda A, Kumar S, Li W, Coleman J, Liu Y, Rothman JE, Bhattacharyya M, Gupta K. bioRxiv. 2024 Aug 4; 2024 Aug 4. PMID: 38405833.
• Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging.Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman JE, Toomre D, Bewersdorf J. Cell. 2024 Mar 28. PMID: 38552613.
• Biallelic NAA60 variants with impaired n-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications.Chelban V, Aksnes H, Maroofian R, LaMonica LC, Seabra L, Siggervåg A, Devic P, Shamseldin HE, Vandrovcova J, Murphy D, Richard AC, Quenez O, Bonnevalle A, Zanetti MN, Kaiyrzhanov R, Salpietro V, Efthymiou S, Schottlaender LV, Morsy H, Scardamaglia A, Tariq A, Pagnamenta AT, Pennavaria A, Krogstad LS, Bekkelund ÅK, Caiella A, Glomnes N, Brønstad KM, Tury S, Moreno De Luca A, Boland-Auge A, Olaso R, Deleuze JF, Anheim M, Cretin B, Vona B, Alajlan F, Abdulwahab F, Battini JL, İpek R, Bauer P, Zifarelli G, Gungor S, Kurul SH, Lochmuller H, Da'as SI, Fakhro KA, Gómez-Pascual A, Botía JA, Wood NW, Horvath R, Ernst AM, Rothman JE, McEntagart M, Crow YJ, Alkuraya FS, Nicolas G, Arnesen T, Houlden H. Nat Commun. 2024 Mar 13; 2024 Mar 13. PMID: 38480682.
• Synaptophysin chaperones the assembly of 12 SNAREpins under each ready-release vesicle.Bera M, Radhakrishnan A, Coleman J, K Sundaram RV, Ramakrishnan S, Pincet F, Rothman JE. Proc Natl Acad Sci U S A. 2023 Nov 7; 2023 Oct 30. PMID: 37903271.
• Diacylglycerol-dependent hexamers of the SNARE-assembling chaperone Munc13-1 cooperatively bind vesicles.Li F, Grushin K, Coleman J, Pincet F, Rothman JE. Proc Natl Acad Sci U S A. 2023 Oct 31; 2023 Oct 26. PMID: 37883433.
• Starting at Go: Protein structure prediction succumbs to machine learning.Rothman JE. Proc Natl Acad Sci U S A. 2023 Sep 26; 2023 Sep 21. PMID: 37732752.
• Turbocharging synaptic transmission.Rothman JE, Grushin K, Bera M, Pincet F. FEBS Lett. 2023 Sep; 2023 Sep 7. PMID: 37643878.
• Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery.Kalyana Sundaram RV, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst AM, Gupta K, Rothman JE, Krishnakumar SS. Proc Natl Acad Sci U S A. 2023 Aug 22; 2023 Aug 17. PMID: 37590407.
• Synaptophysin Chaperones the Assembly of 12 SNAREpins under each Ready-Release Vesicle.Bera M, Radhakrishnan A, Coleman J, Sundaram RVK, Ramakrishnan S, Pincet F, Rothman JE. bioRxiv. 2023 Jul 6; 2023 Jul 6. PMID: 37461465.
• Novel Roles for Diacylglycerol in Synaptic Vesicle Priming and Release Revealed by Complete Reconstitution of Core Protein Machinery.Sundaram RVK, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst AM, Gupta K, Rothman JE, Krishnakumar SS. bioRxiv. 2023 Jun 7; 2023 Jun 7. PMID: 37333317.
• Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer.Panda A, Giska F, Duncan AL, Welch AJ, Brown C, McAllister R, Hariharan P, Goder JND, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman JE, Gupta K. Nat Methods. 2023 Jun; 2023 Apr 27. PMID: 37106230.
• The gripping story of integrins.Rothman JE. Cell. 2022 Oct 13; 2022 Sep 28. PMID: 36174578.
• Synaptotagmin rings as high-sensitivity regulators of synaptic vesicle docking and fusion.Zhu J, McDargh ZA, Li F, Krishnakumar SS, Rothman JE, O'Shaughnessy B. Proc Natl Acad Sci U S A. 2022 Sep 20; 2022 Sep 14. PMID: 36103579.
• Molecular determinants of complexin clamping and activation function.Bera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Elife. 2022 Apr 20; 2022 Apr 20. PMID: 35442188.
• Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release.Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE. Proc Natl Acad Sci U S A. 2022 Feb 15. PMID: 35135883.
• Cooperation of Conical and Polyunsaturated Lipids to Regulate Initiation and Processing of Membrane Fusion.François-Martin C, Bacle A, Rothman JE, Fuchs PFJ, Pincet F. Front Mol Biosci. 2021; 2021 Oct 21. PMID: 34746239.
• Vesicle capture by membrane-bound Munc13-1 requires self-assembly into discrete clusters.Li F, Kalyana Sundaram RV, Gatta AT, Coleman J, Ramakrishnan S, Krishnakumar SS, Pincet F, Rothman JE. FEBS Lett. 2021 Sep; 2021 Jul 24. PMID: 34227103.
• Combining genomic and epidemiological data to compare the transmissibility of SARS-CoV-2 lineages.Petrone ME, Rothman JE, Breban MI, Ott IM, Russell A, Lasek-Nesselquist E, Kelly K, Omerza G, Renzette N, Watkins AE, Kalinich CC, Alpert T, Brito AF, Earnest R, Tikhonova IR, Castaldi C, Kelly JP, Shudt M, Plitnick J, Schneider E, Murphy S, Neal C, Laszlo E, Altajar A, Pearson C, Muyombwe A, Downing R, Razeq J, Niccolai L, Wilson MS, Anderson ML, Wang J, Liu C, Hui P, Mane S, Taylor BP, Hanage WP, Landry ML, Peaper DR, Bilguvar K, Fauver JR, Vogels CBF, Gardner LM, Pitzer VE, St George K, Adams MD, Grubaugh ND. medRxiv. 2021 Jul 2; 2021 Jul 2. PMID: 34230938.
• Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution.Hao X, Allgeyer ES, Lee DR, Antonello J, Watters K, Gerdes JA, Schroeder LK, Bottanelli F, Zhao J, Kidd P, Lessard MD, Rothman JE, Cooley L, Biederer T, Booth MJ, Bewersdorf J. Nat Methods. 2021 Jun; 2021 May 31. PMID: 34059828.
• Nascent fusion pore opening monitored at single-SNAREpin resolution.Heo P, Coleman J, Fleury JB, Rothman JE, Pincet F. Proc Natl Acad Sci U S A. 2021 Feb 2. PMID: 33495324.
• Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals.Radhakrishnan A, Li X, Grushin K, Krishnakumar SS, Liu J, Rothman JE. Proc Natl Acad Sci U S A. 2021 Feb 2. PMID: 33468631.
• Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.Kalyana Sundaram RV, Jin H, Li F, Shu T, Coleman J, Yang J, Pincet F, Zhang Y, Rothman JE, Krishnakumar SS. FEBS Lett. 2021 Feb; 2020 Dec 5. PMID: 33222163.
• Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain.Gruget C, Bello O, Coleman J, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH Jr. Sci Rep. 2020 Oct 22; 2020 Oct 22. PMID: 33093513.
• The golgin family exhibits a propensity to form condensates in living cells.Ziltener P, Rebane AA, Graham M, Ernst AM, Rothman JE. FEBS Lett. 2020 Oct; 2020 Aug 2. PMID: 32668013.
• TANGO1 membrane helices create a lipid diffusion barrier at curved membranes.Raote I, Ernst AM, Campelo F, Rothman JE, Pincet F, Malhotra V. Elife. 2020 May 26; 2020 May 26. PMID: 32452385.
• Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis.Ramakrishnan S, Bera M, Coleman J, Rothman JE, Krishnakumar SS. Elife. 2020 May 13; 2020 May 13. PMID: 32401194.
• Liquid-liquid phase separation of the Golgi matrix protein GM130.Rebane AA, Ziltener P, LaMonica LC, Bauer AH, Zheng H, López-Montero I, Pincet F, Rothman JE, Ernst AM. FEBS Lett. 2020 Apr; 2019 Dec 26. PMID: 31833055.
• Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE. Proc Natl Acad Sci U S A. 2020 Feb 18; 2020 Feb 3. PMID: 32015138.
• Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J. Nat Methods. 2020 Feb; 2020 Jan 6. PMID: 31907447.
• Munc13-1 MUN domain and Munc18-1 cooperatively chaperone SNARE assembly through a tetrameric complex.Shu T, Jin H, Rothman JE, Zhang Y. Proc Natl Acad Sci U S A. 2020 Jan 14; 2019 Dec 30. PMID: 31888993.
• Jim's View: Is the Golgi stack a phase-separated liquid crystal?Rothman JE. FEBS Lett. 2019 Oct; 2019 Sep 27. PMID: 31562650.
• Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack.Tiwari N, Graham M, Liu X, Yue X, Zhu L, Meshram D, Choi S, Qian Y, Rothman JE, Lee I. Sci Rep. 2019 Aug 28; 2019 Aug 28. PMID: 31462665.
• Jim's view: What makes transformative basic science possible?Rothman JE. FEBS Lett. 2019 Aug; 2019 Jul 12. PMID: 31301063.
• Jim's view: Patience vs urgency.Rothman JE. FEBS Lett. 2019 Aug; 2019 Jul 12. PMID: 31301062.
• PDXK mutations cause polyneuropathy responsive to pyridoxal 5'-phosphate supplementation.Chelban V, Wilson MP, Warman Chardon J, Vandrovcova J, Zanetti MN, Zamba-Papanicolaou E, Efthymiou S, Pope S, Conte MR, Abis G, Liu YT, Tribollet E, Haridy NA, Botía JA, Ryten M, Nicolaou P, Minaidou A, Christodoulou K, Kernohan KD, Eaton A, Osmond M, Ito Y, Bourque P, Jepson JEC, Bello O, Bremner F, Cordivari C, Reilly MM, Foiani M, Heslegrave A, Zetterberg H, Heales SJR, Wood NW, Rothman JE, Boycott KM, Mills PB, Clayton PT, Houlden H. Ann Neurol. 2019 Aug; 2019 Jul 1. PMID: 31187503.
• AMPA receptor GluA2 subunit defects are a cause of neurodevelopmental disorders.Salpietro V, Dixon CL, Guo H, Bello OD, Vandrovcova J, Efthymiou S, Maroofian R, Heimer G, Burglen L, Valence S, Torti E, Hacke M, Rankin J, Tariq H, Colin E, Procaccio V, Striano P, Mankad K, Lieb A, Chen S, Pisani L, Bettencourt C, Männikkö R, Manole A, Brusco A, Grosso E, Ferrero GB, Armstrong-Moron J, Gueden S, Bar-Yosef O, Tzadok M, Monaghan KG, Santiago-Sim T, Person RE, Cho MT, Willaert R, Yoo Y, Chae JH, Quan Y, Wu H, Wang T, Bernier RA, Xia K, Blesson A, Jain M, Motazacker MM, Jaeger B, Schneider AL, Boysen K, Muir AM, Myers CT, Gavrilova RH, Gunderson L, Schultz-Rogers L, Klee EW, Dyment D, Osmond M, Parellada M, Llorente C, Gonzalez-Peñas J, Carracedo A, Van Haeringen A, Ruivenkamp C, Nava C, Heron D, Nardello R, Iacomino M, Minetti C, Skabar A, Fabretto A, Raspall-Chaure M, Chez M, Tsai A, Fassi E, Shinawi M, Constantino JN, De Zorzi R, Fortuna S, Kok F, Keren B, Bonneau D, Choi M, Benzeev B, Zara F, Mefford HC, Scheffer IE, Clayton-Smith J, Macaya A, Rothman JE, Eichler EE, Kullmann DM, Houlden H. Nat Commun. 2019 Jul 12; 2019 Jul 12. PMID: 31300657.
• Jim's view: Why basic science?Rothman JE. FEBS Lett. 2019 Jul; 2019 Jul 12. PMID: 31301070.
• Structural basis for the clamping and Ca(2+) activation of SNARE-mediated fusion by synaptotagmin.Grushin K, Wang J, Coleman J, Rothman JE, Sindelar CV, Krishnakumar SS. Nat Commun. 2019 Jun 3; 2019 Jun 3. PMID: 31160571.
• Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D-Printed Microfluidic Setup.Heo P, Ramakrishnan S, Coleman J, Rothman JE, Fleury JB, Pincet F. Small. 2019 May; 2019 Apr 12. PMID: 30977975.
• Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags.Erdmann RS, Baguley SW, Richens JH, Wissner RF, Xi Z, Allgeyer ES, Zhong S, Thompson AD, Lowe N, Butler R, Bewersdorf J, Rothman JE, St Johnston D, Schepartz A, Toomre D. Cell Chem Biol. 2019 Apr 18; 2019 Feb 7. PMID: 30745239.
• Mutations in the Neuronal Vesicular SNARE VAMP2 Affect Synaptic Membrane Fusion and Impair Human Neurodevelopment.Salpietro V, Malintan NT, Llano-Rivas I, Spaeth CG, Efthymiou S, Striano P, Vandrovcova J, Cutrupi MC, Chimenz R, David E, Di Rosa G, Marce-Grau A, Raspall-Chaure M, Martin-Hernandez E, Zara F, Minetti C, Bello OD, De Zorzi R, Fortuna S, Dauber A, Alkhawaja M, Sultan T, Mankad K, Vitobello A, Thomas Q, Mau-Them FT, Faivre L, Martinez-Azorin F, Prada CE, Macaya A, Kullmann DM, Rothman JE, Krishnakumar SS, Houlden H. Am J Hum Genet. 2019 Apr 4; 2019 Mar 28. PMID: 30929742.
• SNARE machinery is optimized for ultrafast fusion.Manca F, Pincet F, Truskinovsky L, Rothman JE, Foret L, Caruel M. Proc Natl Acad Sci U S A. 2019 Feb 12; 2019 Jan 30. PMID: 30700546.
• Synaptotagmin oligomers are necessary and can be sufficient to form a Ca(2+) -sensitive fusion clamp.Ramakrishnan S, Bera M, Coleman J, Krishnakumar SS, Pincet F, Rothman JE. FEBS Lett. 2019 Jan; 2019 Jan 18. PMID: 30570144.
• Symmetrical organization of proteins under docked synaptic vesicles.Li X, Radhakrishnan A, Grushin K, Kasula R, Chaudhuri A, Gomathinayagam S, Krishnakumar SS, Liu J, Rothman JE. FEBS Lett. 2019 Jan; 2019 Jan 18. PMID: 30561792.
• Jim's view: analog to digital conversion in biology.Rothman JE. FEBS Lett. 2018 Dec; 2018 Dec 15. PMID: 30552677.
• S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi.Ernst AM, Syed SA, Zaki O, Bottanelli F, Zheng H, Hacke M, Xi Z, Rivera-Molina F, Graham M, Rebane AA, Björkholm P, Baddeley D, Toomre D, Pincet F, Rothman JE. Dev Cell. 2018 Nov 19. PMID: 30458139.
• FEBS Letters Special Issue on Exocytosis and Endocytosis.Karatekin E, Rothman JE. FEBS Lett. 2018 Nov. PMID: 30417372.
• Assessing photodamage in live-cell STED microscopy.Kilian N, Goryaynov A, Lessard MD, Hooker G, Toomre D, Rothman JE, Bewersdorf J. Nat Methods. 2018 Oct. PMID: 30275592.
• Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS. Proc Natl Acad Sci U S A. 2018 Aug 7; 2018 Jul 23. PMID: 30038018.
• Jim's View: "Playing Billiards with Science".Rothman JE. FEBS Lett. 2018 Jul; 2018 Jun 19. PMID: 29920662.
• High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis.Ramakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. Langmuir. 2018 May 22; 2018 May 9. PMID: 29694054.
• Rearrangements under confinement lead to increased binding energy of Synaptotagmin-1 with anionic membranes in Mg(2+) and Ca(2).Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH Jr. FEBS Lett. 2018 May; 2018 Apr 10. PMID: 29578584.
• Two Disease-Causing SNAP-25B Mutations Selectively Impair SNARE C-terminal Assembly.Rebane AA, Wang B, Ma L, Qu H, Coleman J, Krishnakumar S, Rothman JE, Zhang Y. J Mol Biol. 2018 Feb 16; 2017 Oct 19. PMID: 29056461.
• Jim's View: "Some Thoughts for Young Scientists".Rothman JE. FEBS Lett. 2018 Feb; 2018 Jan 15. PMID: 29336035.
• PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex Assembly.Coleman J, Jouannot O, Ramakrishnan SK, Zanetti MN, Wang J, Salpietro V, Houlden H, Rothman JE, Krishnakumar SS. Cell Rep. 2018 Jan 16. PMID: 29346777.
• Otoferlin acts as a Ca(2+) sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.Michalski N, Goutman JD, Auclair SM, Boutet de Monvel J, Tertrais M, Emptoz A, Parrin A, Nouaille S, Guillon M, Sachse M, Ciric D, Bahloul A, Hardelin JP, Sutton RB, Avan P, Krishnakumar SS, Rothman JE, Dulon D, Safieddine S, Petit C. Elife. 2017 Nov 7; 2017 Nov 7. PMID: 29111973.
• Hypothesis - buttressed rings assemble, clamp, and release SNAREpins for synaptic transmission.Rothman JE, Krishnakumar SS, Grushin K, Pincet F. FEBS Lett. 2017 Nov; 2017 Oct 31. PMID: 28983915.
• Mutations in Membrin/GOSR2 Reveal Stringent Secretory Pathway Demands of Dendritic Growth and Synaptic Integrity.Praschberger R, Lowe SA, Malintan NT, Giachello CNG, Patel N, Houlden H, Kullmann DM, Baines RA, Usowicz MM, Krishnakumar SS, Hodge JJL, Rothman JE, Jepson JEC. Cell Rep. 2017 Oct 3. PMID: 28978487.
• Land-locked mammalian Golgi reveals cargo transport between stable cisternae.Dunlop MH, Ernst AM, Schroeder LK, Toomre DK, Lavieu G, Rothman JE. Nat Commun. 2017 Sep 5; 2017 Sep 5. PMID: 28874656.
• ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP.Yue X, Bao M, Christiano R, Li S, Mei J, Zhu L, Mao F, Yue Q, Zhang P, Jing S, Rothman JE, Qian Y, Lee I. FEBS Lett. 2017 Sep; 2017 Aug 21. PMID: 28777890.
• Circular oligomerization is an intrinsic property of synaptotagmin.Wang J, Li F, Bello OD, Sindelar CV, Pincet F, Krishnakumar SS, Rothman JE. Elife. 2017 Aug 29; 2017 Aug 29. PMID: 28850328.
• Long time-lapse nanoscopy with spontaneously blinking membrane probes.Takakura H, Zhang Y, Erdmann RS, Thompson AD, Lin Y, McNellis B, Rivera-Molina F, Uno SN, Kamiya M, Urano Y, Rothman JE, Bewersdorf J, Schepartz A, Toomre D. Nat Biotechnol. 2017 Aug; 2017 Jul 3. PMID: 28671662.
• A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi.Bottanelli F, Kilian N, Ernst AM, Rivera-Molina F, Schroeder LK, Kromann EB, Lessard MD, Erdmann RS, Schepartz A, Baddeley D, Bewersdorf J, Toomre D, Rothman JE. Mol Biol Cell. 2017 Jun 15; 2017 Apr 20. PMID: 28428254.
• Mutations in NKX6-2 Cause Progressive Spastic Ataxia and Hypomyelination.Chelban V, Patel N, Vandrovcova J, Zanetti MN, Lynch DS, Ryten M, Botía JA, Bello O, Tribollet E, Efthymiou S, Davagnanam I, Bashiri FA, Wood NW, Rothman JE, Alkuraya FS, Houlden H. Am J Hum Genet. 2017 Jun 1. PMID: 28575651.
• Entropic forces drive self-organization and membrane fusion by SNARE proteins.Mostafavi H, Thiyagarajan S, Stratton BS, Karatekin E, Warner JM, Rothman JE, O'Shaughnessy B. Proc Natl Acad Sci U S A. 2017 May 23; 2017 May 10. PMID: 28490503.
• Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.Salpietro V, Lin W, Delle Vedove A, Storbeck M, Liu Y, Efthymiou S, Manole A, Wiethoff S, Ye Q, Saggar A, McElreavey K, Krishnakumar SS, Pitt M, Bello OD, Rothman JE, Basel-Vanagaite L, Hubshman MW, Aharoni S, Manzur AY, Wirth B, Houlden H. Ann Neurol. 2017 Apr; 2017 Mar 29. PMID: 28253535.
• Low energy cost for optimal speed and control of membrane fusion.François-Martin C, Rothman JE, Pincet F. Proc Natl Acad Sci U S A. 2017 Feb 7; 2017 Jan 23. PMID: 28115718.
• Stability, folding dynamics, and long-range conformational transition of the synaptic t-SNARE complex.Zhang X, Rebane AA, Ma L, Li F, Jiao J, Qu H, Pincet F, Rothman JE, Zhang Y. Proc Natl Acad Sci U S A. 2016 Dec 13; 2016 Nov 28. PMID: 27911771.
• Kinetic barriers to SNAREpin assembly in the regulation of membrane docking/priming and fusion.Li F, Tiwari N, Rothman JE, Pincet F. Proc Natl Acad Sci U S A. 2016 Sep 20; 2016 Sep 6. PMID: 27601655.
• Ultra-High Resolution 3D Imaging of Whole Cells.Huang F, Sirinakis G, Allgeyer ES, Schroeder LK, Duim WC, Kromann EB, Phan T, Rivera-Molina FE, Myers JR, Irnov I, Lessard M, Zhang Y, Handel MA, Jacobs-Wagner C, Lusk CP, Rothman JE, Toomre D, Booth MJ, Bewersdorf J. Cell. 2016 Aug 11; 2016 Jul 7. PMID: 27397506.
• Ring-like oligomers of Synaptotagmins and related C2 domain proteins.Zanetti MN, Bello OD, Wang J, Coleman J, Cai Y, Sindelar CV, Rothman JE, Krishnakumar SS. Elife. 2016 Jul 19; 2016 Jul 19. PMID: 27434670.
• FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments.Pincet F, Adrien V, Yang R, Delacotte J, Rothman JE, Urbach W, Tareste D. PLoS One. 2016; 2016 Jul 7. PMID: 27387979.
• Small cargoes pass through synthetically glued Golgi stacks.Dancourt J, Zheng H, Bottanelli F, Allgeyer ES, Bewersdorf J, Graham M, Liu X, Rothman JE, Lavieu G. FEBS Lett. 2016 Jun; 2016 May 31. PMID: 27174538.
• Self-assembly of size-controlled liposomes on DNA nanotemplates.Yang Y, Wang J, Shigematsu H, Xu W, Shih WM, Rothman JE, Lin C. Nat Chem. 2016 May; 2016 Mar 21. PMID: 27102682.
• A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion.Xu W, Nathwani B, Lin C, Wang J, Karatekin E, Pincet F, Shih W, Rothman JE. J Am Chem Soc. 2016 Apr 6; 2016 Mar 23. PMID: 26938705.
• Using ApoE Nanolipoprotein Particles To Analyze SNARE-Induced Fusion Pores.Bello OD, Auclair SM, Rothman JE, Krishnakumar SS. Langmuir. 2016 Mar 29; 2016 Mar 18. PMID: 26972604.
• Two-colour live-cell nanoscale imaging of intracellular targets.Bottanelli F, Kromann EB, Allgeyer ES, Erdmann RS, Wood Baguley S, Sirinakis G, Schepartz A, Baddeley D, Toomre DK, Rothman JE, Bewersdorf J. Nat Commun. 2016 Mar 4; 2016 Mar 4. PMID: 26940217.
• Accelerating SNARE-Mediated Membrane Fusion by DNA-Lipid Tethers.Xu W, Wang J, Rothman JE, Pincet F. Angew Chem Int Ed Engl. 2015 Nov 23; 2015 Oct 6. PMID: 26439984.
• Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock.Motta I, Gohlke A, Adrien V, Li F, Gardavot H, Rothman JE, Pincet F. Langmuir. 2015 Jun 30; 2015 Jun 15. PMID: 26038815.
• Re-visiting the trans insertion model for complexin clamping.Krishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Elife. 2015 Apr 2; 2015 Apr 2. PMID: 25831964.
• The principle of membrane fusion in the cell (Nobel lecture).Rothman JE. Angew Chem Int Ed Engl. 2014 Nov 17; 2014 Aug 1. PMID: 25087728.
• The Golgi ribbon structure facilitates anterograde transport of large cargoes.Lavieu G, Dunlop MH, Lerich A, Zheng H, Bottanelli F, Rothman JE. Mol Biol Cell. 2014 Oct 1; 2014 Aug 7. PMID: 25103235.
• Calcium sensitive ring-like oligomers formed by synaptotagmin.Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Proc Natl Acad Sci U S A. 2014 Sep 23; 2014 Sep 8. PMID: 25201968.
• Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins.Zorman S, Rebane AA, Ma L, Yang G, Molski MA, Coleman J, Pincet F, Rothman JE, Zhang Y. Elife. 2014 Sep 1; 2014 Sep 1. PMID: 25180101.
• Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo.Cho RW, Kümmel D, Li F, Baguley SW, Coleman J, Rothman JE, Littleton JT. Proc Natl Acad Sci U S A. 2014 Jul 15; 2014 Jun 30. PMID: 24982161.
• A half-zippered SNARE complex represents a functional intermediate in membrane fusion.Li F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F. J Am Chem Soc. 2014 Mar 5; 2014 Feb 18. PMID: 24533674.
• Membrane adhesion dictates Golgi stacking and cisternal morphology.Lee I, Tiwari N, Dunlop MH, Graham M, Liu X, Rothman JE. Proc Natl Acad Sci U S A. 2014 Feb 4; 2014 Jan 21. PMID: 24449908.
• Conformational dynamics of calcium-triggered activation of fusion by synaptotagmin.Krishnakumar SS, Kümmel D, Jones SJ, Radoff DT, Reinisch KM, Rothman JE. Biophys J. 2013 Dec 3. PMID: 24314081.
• Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes.Pellett PA, Dietrich F, Bewersdorf J, Rothman JE, Lavieu G. Elife. 2013 Oct 1; 2013 Oct 1. PMID: 24137546.
• COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function.Thiam AR, Antonny B, Wang J, Delacotte J, Wilfling F, Walther TC, Beck R, Rothman JE, Pincet F. Proc Natl Acad Sci U S A. 2013 Aug 13; 2013 Jul 30. PMID: 23901109.
• Stapled Golgi cisternae remain in place as cargo passes through the stack.Lavieu G, Zheng H, Rothman JE. Elife. 2013 Jun 4; 2013 Jun 4. PMID: 23755362.
• Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores.Shi L, Howan K, Shen QT, Wang YJ, Rothman JE, Pincet F. Nat Protoc. 2013 May; 2013 Apr 18. PMID: 23598444.
• Single reconstituted neuronal SNARE complexes zipper in three distinct stages.Gao Y, Zorman S, Gundersen G, Xi Z, Ma L, Sirinakis G, Rothman JE, Zhang Y. Science. 2012 Sep 14; 2012 Aug 16. PMID: 22903523.
• Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells.Karatekin E, Rothman JE. Nat Protoc. 2012 Apr 19; 2012 Apr 19. PMID: 22517259.
• SNARE proteins: one to fuse and three to keep the nascent fusion pore open.Shi L, Shen QT, Kiel A, Wang J, Wang HW, Melia TJ, Rothman JE, Pincet F. Science. 2012 Mar 16. PMID: 22422984.
• Molecular mechanism of protein folding in the cell.Rothman JE, Schekman R. Cell. 2011 Sep 16; 2011 Sep 9. PMID: 21907398.
• Complexin cross-links prefusion SNAREs into a zigzag array.Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Nat Struct Mol Biol. 2011 Jul 24; 2011 Jul 24. PMID: 21785414.
• Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic state.Li F, Pincet F, Perez E, Giraudo CG, Tareste D, Rothman JE. Nat Struct Mol Biol. 2011 Jul 24; 2011 Jul 24. PMID: 21785413.
• A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion.Krishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. Nat Struct Mol Biol. 2011 Jul 24; 2011 Jul 24. PMID: 21785412.
• The future of Golgi research.Rothman JE. Mol Biol Cell. 2010 Nov 15. PMID: 21079007.