2001
Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge
Kahn J, Roberts A, Weibel C, Buonocore L, Rose J. Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge. Journal Of Virology 2001, 75: 11079-11087. PMID: 11602747, PMCID: PMC114687, DOI: 10.1128/jvi.75.22.11079-11087.2001.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, IntranasalAnimalsAntigens, ViralBase SequenceCell LineCricetinaeFemaleMiceMice, Inbred BALB CMolecular Sequence DataRespiratory Syncytial Virus InfectionsVaccines, AttenuatedVaccines, SyntheticVesicular stomatitis Indiana virusViral ProteinsViral VaccinesVirus ReplicationConceptsRespiratory syncytial virusRSV challengeRecombinant vesicular stomatitis virusVesicular stomatitis virusRSV FRespiratory syncytial virus GRSV-specific antibodiesDetectable neutralizing antibodiesDetectable serum antibodiesStomatitis virusIntranasal vaccinationRSV vaccineRSV GProtective immunitySyncytial virusDetectable serumNeutralizing antibodiesSerum antibodiesMouse modelRSV proteinsComplete protectionReplication competentAntibodiesG glycoproteinVirus
2000
Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice
Grigera P, Marzocca M, Capozzo A, Buonocore L, Donis R, Rose J. Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice. Virus Research 2000, 69: 3-15. PMID: 10989181, DOI: 10.1016/s0168-1702(00)00164-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, ViralBase SequenceCattleCell LineChimeraCricetinaeDiarrhea Viruses, Bovine ViralDNA, RecombinantFemaleMembrane GlycoproteinsMiceMice, Inbred BALB CNeutralization TestsRecombinant ProteinsVaccines, SyntheticVesicular stomatitis Indiana virusViral Envelope ProteinsViral VaccinesConceptsBovine viral diarrhea virus (BVDV) E2BALB/c miceRecombinant vesicular stomatitis virusViral-induced diseasesC miceE2 antibodiesBVDV antibodiesVesicular stomatitis virusHigh avidityBVDV E2Recombinant E2Infected cellsDays post inoculationG glycoproteinE2Stomatitis virusMiceAntibodiesImmunofluorescence microscopyPost inoculationRecombinant particlesBHK21 cellsCellsSymptomsInfection
1997
High-efficiency incorporation of functional influenza virus glycoproteins into recombinant vesicular stomatitis viruses
Kretzschmar E, Buonocore L, Schnell M, Rose J. High-efficiency incorporation of functional influenza virus glycoproteins into recombinant vesicular stomatitis viruses. Journal Of Virology 1997, 71: 5982-5989. PMID: 9223488, PMCID: PMC191854, DOI: 10.1128/jvi.71.8.5982-5989.1997.Peer-Reviewed Original ResearchConceptsVSV G proteinVesicular stomatitis virusG proteinsForeign membrane proteinsForeign proteinsVirus membrane glycoproteinsVSV particlesVSV G geneNA proteinsStomatitis virusGene orderExtra genesVSV transcriptionMembrane proteinsRecombinant vesicular stomatitis virusSite upstreamVSV virionsVSV membraneHA proteinInfluenza virus proteinsInfluenza virus glycoproteinsGlycoprotein geneGlycoprotein CD4Membrane glycoproteinsViral genome
1996
Membrane Association of Influenza Virus Matrix Protein Does Not Require Specific Hydrophobic Domains or the Viral Glycoproteins
KRETZSCHMAR E, BUI M, ROSE J. Membrane Association of Influenza Virus Matrix Protein Does Not Require Specific Hydrophobic Domains or the Viral Glycoproteins. Virology 1996, 220: 37-45. PMID: 8659126, DOI: 10.1006/viro.1996.0283.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBinding SitesCell LineCell MembraneChick EmbryoDogsHeLa CellsHemagglutinin Glycoproteins, Influenza VirusHemagglutinins, ViralHumansInfluenza A virusMolecular Sequence DataMutagenesis, Site-DirectedNeuraminidaseOligodeoxyribonucleotidesRecombinant ProteinsViral Matrix ProteinsConceptsMembrane associationSpecific hydrophobic domainsM1 proteinMatrix proteinsHydrophobic domainInfluenza virus matrix proteinVirus matrix proteinInteraction of M1Viral glycoproteinsMajor structural componentRibonucleocapsid coreCytoplasmic tailIntegral proteinsMembrane proteinsMembrane bindingSubcellular fractionationMembrane envelopeCellular membranesHeLa cellsViral proteinsHydrophobic regionProteinIsolated membranesMembraneInfluenza proteinsExpression of Additional Genes in a Vector Derived from a Minimal RNA Virus
ROLLS M, HAGLUND K, ROSE J. Expression of Additional Genes in a Vector Derived from a Minimal RNA Virus. Virology 1996, 218: 406-411. PMID: 8610469, DOI: 10.1006/viro.1996.0211.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCapsidCD4 AntigensCell LineChloramphenicol O-AcetyltransferaseCloning, MolecularCricetinaeGene ExpressionGenetic VectorsMembrane GlycoproteinsMolecular Sequence DataPromoter Regions, GeneticRecombinant Fusion ProteinsRNA, ViralSemliki forest virusVesicular stomatitis Indiana virusViral Core ProteinsViral Envelope ProteinsConceptsVSV G proteinG proteinsVSV G geneVesicular stomatitis virus glycoproteinTotal cell proteinMembrane fusion activityViral structural proteinsHost protein synthesisTissue culture cellsUnselected genesMultiple cloning siteInfectious particlesAdditional genesForeign genesPlasma membraneG RNADifferent proteinsStructural proteinsMajor proteinsRNA repliconsCell proteinsFusion activityRNA virusesProtein synthesisGenesCharacterization of a Nuclear Protein Conferring Brefeldin A Resistance in Schizosaccharomyces pombe (∗)
Turi T, Mueller U, Sazer S, Rose J. Characterization of a Nuclear Protein Conferring Brefeldin A Resistance in Schizosaccharomyces pombe (∗). Journal Of Biological Chemistry 1996, 271: 9166-9171. PMID: 8621569, DOI: 10.1074/jbc.271.15.9166.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntifungal AgentsBase SequenceBrefeldin ACell CompartmentationConsensus SequenceCyclopentanesDNA PrimersDrug Resistance, MicrobialFungal ProteinsGenes, FungalGolgi ApparatusGTP-Binding ProteinsMolecular Sequence DataNuclear ProteinsPhosphoproteinsRan GTP-Binding ProteinRestriction MappingSchizosaccharomycesSequence AlignmentSequence Homology, Amino AcidConceptsNuclear pore complexWild type SchizosaccharomycesPore complexS. pombeSchizosaccharomyces pombeProtein RanBP1Essential proteinsGolgi complexEndoplasmic reticulumProtein secretionPeptide motifsMultiple copiesNovel mechanismGenesProteinPombeA ResistanceBrefeldinDrug resistanceSchizosaccharomycesYrb1RanBP1HomologyComplexesReticulumThe minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus
Schnell M, Buonocore L, Whitt M, Rose J. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. Journal Of Virology 1996, 70: 2318-2323. PMID: 8642658, PMCID: PMC190073, DOI: 10.1128/jvi.70.4.2318-2323.1996.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceChloramphenicol O-AcetyltransferaseCloning, MolecularConserved SequenceGene Expression Regulation, ViralMembrane GlycoproteinsMolecular Sequence DataRegulatory Sequences, Nucleic AcidRNA, ViralSerial PassageTranscription, GeneticVesicular stomatitis Indiana virusViral Envelope ProteinsViral Plaque AssayConceptsForeign genesBacterial enzyme chloramphenicolNew transcription unitTranscription start sequenceWild-type titersTranscription unitFunctional chloramphenicolPolyadenylation signalEnzyme chloramphenicolCell biologyVesicular stomatitis virusVSV genomeIntergenic dinucleotideGene junctionStable expressionGlycoprotein geneGenesDNA copiesGlycoprotein mRNAInfectious VSVRecombinant virusesStomatitis virusStart sequenceRecombinant VSVSequenceNormal Replication of Vesicular Stomatitis Virus without C Proteins
KRETZSCHMAR E, PELUSO R, SCHNELL M, WHITT M, ROSE J. Normal Replication of Vesicular Stomatitis Virus without C Proteins. Virology 1996, 216: 309-316. PMID: 8607260, DOI: 10.1006/viro.1996.0066.Peer-Reviewed Original ResearchConceptsP geneWild-type virusNormal replicationSmall basic proteinP protein sequenceSingle base changeNew Jersey serotypeWild-type virus particlesInsect vectorsVesicular stomatitis virusC proteinStop codonViral mRNAsVSV growthInfectious cloneBase changesMutant virusProteinGenesStomatitis virusViral pathogenesisBasic proteinM proteinVirus particlesTissue culture
1995
Characterization of a Novel Schizosaccharomyces pombe Multidrug Resistance Transporter Conferring Brefeldin A Resistance
Turi T, Rose J. Characterization of a Novel Schizosaccharomyces pombe Multidrug Resistance Transporter Conferring Brefeldin A Resistance. Biochemical And Biophysical Research Communications 1995, 213: 410-418. PMID: 7646493, DOI: 10.1006/bbrc.1995.2147.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceATP-Binding Cassette TransportersBase SequenceBinding SitesBrefeldin ACloning, MolecularCyclopentanesDNA, FungalDrug Resistance, MultipleFungal ProteinsMolecular Sequence DataOpen Reading FramesPancreatitis-Associated ProteinsRestriction MappingSchizosaccharomycesSchizosaccharomyces pombe ProteinsSequence AnalysisSequence HomologyConceptsTranscription factorsHbA2 expressionWild-type S. pombeS. pombe proteinPap1 transcription factorSignificant sequence similarityWild type SchizosaccharomycesMultiple transcription factorsPombe proteinsCRM1 genesS. pombeSequence similarityMammalian cellsTransport proteinsDifferent genesMutant strainBrefeldin AGolgi complexEndoplasmic reticulumProtein secretionGenesMultiple copiesSchizosaccharomycesCRM1A ResistanceRecombinant vesicular stomatitis viruses from DNA.
Lawson N, Stillman E, Whitt M, Rose J. Recombinant vesicular stomatitis viruses from DNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4477-4481. PMID: 7753828, PMCID: PMC41967, DOI: 10.1073/pnas.92.10.4477.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCapsidCell LineCricetinaeDNA PrimersDNA, ViralDNA-Directed RNA PolymerasesGenome, ViralKidneyMacromolecular SubstancesMolecular Sequence DataPolymerase Chain ReactionRecombination, GeneticRestriction MappingRNA, ViralTransfectionVesicular stomatitis Indiana virusViral Core ProteinsConceptsVesicular stomatitis virusBacteriophage T7 RNA polymeraseVSV nucleocapsid proteinT7 RNA polymeraseStomatitis virusPositive-strand RNAWild-type vesicular stomatitis virusPolymerase subunitsGenetic tagsRNA polymeraseDNA clonesRecombinant vesicular stomatitis virusGenomic RNAVSV genomeGenetic analysisRestriction sitesPrototype rhabdovirusNucleocapsid proteinDNARNAVSV replicationGenomeVSV recombinantsModified virusRecovered virusReplication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins
Stillman E, Rose J, Whitt M. Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. Journal Of Virology 1995, 69: 2946-2953. PMID: 7707520, PMCID: PMC188993, DOI: 10.1128/jvi.69.5.2946-2953.1995.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virusViral structural proteinsStructural proteinsMatrix protein geneFunctional viral proteinsM proteinNorthern blot analysisEncoded proteinsInfectious particlesTranscriptional eventsFunctional mRNAProtein geneL proteinLeader regionMinigenome RNAVSV genomeIndiana serotypePolymerase proteinOwn propagationGlycoprotein geneVSV proteinsViral proteinsProteinNucleocapsid proteinMinigenome
1994
Stimulation of Heterologous Protein Degradation by the Vpu Protein of HIV-1 Requires the Transmembrane and Cytoplasmic Domains of CD4
Buonocore L, Turi T, Crise B, Rose J. Stimulation of Heterologous Protein Degradation by the Vpu Protein of HIV-1 Requires the Transmembrane and Cytoplasmic Domains of CD4. Virology 1994, 204: 482-486. PMID: 8091684, DOI: 10.1006/viro.1994.1560.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCD4 AntigensGlycoproteinsHeLa CellsHIV-1Human Immunodeficiency Virus ProteinsHumansMembrane GlycoproteinsMolecular Sequence DataProtein Structure, TertiaryRecombinant Fusion ProteinsRecombinant ProteinsViral Envelope ProteinsViral Regulatory and Accessory ProteinsConceptsCytoplasmic domainTransmembrane domainHybrid proteinHeterologous protein degradationVesicular stomatitis virus glycoproteinRapid degradationAdditional hybridsProtein degradationExtracellular domainProtein VpuRelated sequencesVpu proteinDegradation systemEndoplasmic reticulumVSV GVpu expressionProteinVpuTransmembraneVirus glycoproteinRecent studiesDomainHuman immunodeficiency virus type 1Immunodeficiency virus type 1DegradationSignals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.
Rodgers W, Crise B, Rose J. Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. PMID: 8035816, PMCID: PMC359057, DOI: 10.1128/mcb.14.8.5384.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCell CompartmentationDNA PrimersGlycolipidsGlycosylphosphatidylinositolsHeLa CellsHumansLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedMyristatesPalmitatesProteinsProtein-Tyrosine KinasesStructure-Activity RelationshipConceptsProtein tyrosine kinasesCertain protein tyrosine kinasesTyrosine kinaseMembrane fractionSrc family protein tyrosine kinasesFamily protein tyrosine kinasesAnalysis of mutantsN-terminal myristateCy-3Glycolipid-enriched membranesAssociation of p56lckCys-5Membrane domainsMembrane proteinsAnchored proteinsGPI anchorGlycosyl phosphatidylinositolKinaseP56lckCell typesHeLa cellsMDCK cellsGPIProteinGentle disruptionInteractions of normal and mutant vesicular stomatitis virus matrix proteins with the plasma membrane and nucleocapsids
Chong L, Rose J. Interactions of normal and mutant vesicular stomatitis virus matrix proteins with the plasma membrane and nucleocapsids. Journal Of Virology 1994, 68: 441-447. PMID: 8254754, PMCID: PMC236304, DOI: 10.1128/jvi.68.1.441-447.1994.Peer-Reviewed Original ResearchConceptsMembrane associationPlasma membraneAmino-terminal basic domainVesicular stomatitis virusWild-type M proteinCellular membranesMatrix proteinsVesicular stomatitis virus matrix proteinM proteinStable membrane associationTruncated M proteinsVirus matrix proteinVSV nucleocapsidsBasic domainAmino terminusNucleocapsid bindingNucleocapsid interactionMembrane fractionVSV proteinsAmino acidsMembrane specificityProteinStomatitis virusNucleocapsidsMembraneMutations in the membrane-spanning domain of the human immunodeficiency virus envelope glycoprotein that affect fusion activity
Owens R, Burke C, Rose J. Mutations in the membrane-spanning domain of the human immunodeficiency virus envelope glycoprotein that affect fusion activity. Journal Of Virology 1994, 68: 570-574. PMID: 8254774, PMCID: PMC236324, DOI: 10.1128/jvi.68.1.570-574.1994.Peer-Reviewed Original ResearchConceptsTransmembrane domainFusion activityVesicular stomatitis virus G proteinMembrane-spanning domainsCell surfaceSpecific amino acid sequencesAmino acid sequenceMembrane fusion activityAmino acid residuesMembrane fusion processCytoplasmic tail domainVirus G proteinCytoplasmic domainMutagenic analysisAcid sequenceChimeric proteinBasic residuesProtein ectodomainAcid residuesG proteinsHeLa cellsVirus envelope glycoproteinLipid bilayersProteinGp41 transmembrane
1993
Cytoplasmic domain requirement for incorporation of a foreign envelope protein into vesicular stomatitis virus
Owens R, Rose J. Cytoplasmic domain requirement for incorporation of a foreign envelope protein into vesicular stomatitis virus. Journal Of Virology 1993, 67: 360-365. PMID: 8093220, PMCID: PMC237371, DOI: 10.1128/jvi.67.1.360-365.1993.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCD4-Positive T-LymphocytesCell FusionFluorescent Antibody TechniqueGene Products, envHIV Envelope Protein gp120HIV Envelope Protein gp41HIV-1Membrane GlycoproteinsMolecular Sequence DataRecombinant Fusion ProteinsStructure-Activity RelationshipVesicular stomatitis Indiana virusViral Envelope ProteinsViral Fusion ProteinsConceptsHIV-1 envelope proteinEnvelope proteinAnti-HIV-1 seraHuman immunodeficiency virus type 1 (HIV-1) envelope proteinG proteinsHIV-1 entryVesicular stomatitis virus particlesHIV-1Vesicular stomatitis virusEnvelope glycoproteinWild-type G proteinStomatitis virusVSV particlesVSV G proteinVirus particlesTemperature-sensitive mutantPseudotypesSimultaneous expressionTransmembrane domainCytoplasmic domainCytoplasmic tailDefective transportVSV
1992
Identification of palmitoylation sites on CD4, the human immunodeficiency virus receptor.
Crise B, Rose J. Identification of palmitoylation sites on CD4, the human immunodeficiency virus receptor. Journal Of Biological Chemistry 1992, 267: 13593-13597. PMID: 1618861, DOI: 10.1016/s0021-9258(18)42253-3.Peer-Reviewed Original ResearchConceptsCytoplasmic domainBinding of p56lckHuman immunodeficiency virus receptorCell surface glycoprotein CD4Palmitoylation sitesCysteine residuesThioester linkageGlycoprotein CD4HeLa cellsCell surfaceVirus receptorProteinFatty acidsMutationsCysteineExpression of CD4Cys397Palmitic acidCys394P56lckTransmembraneCD4AcidPalmitateDomainFc receptor endocytosis is controlled by a cytoplasmic domain determinant that actively prevents coated pit localization.
Miettinen H, Matter K, Hunziker W, Rose J, Mellman I. Fc receptor endocytosis is controlled by a cytoplasmic domain determinant that actively prevents coated pit localization. Journal Of Cell Biology 1992, 116: 875-888. PMID: 1734021, PMCID: PMC2289334, DOI: 10.1083/jcb.116.4.875.Peer-Reviewed Original Research
1991
Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoprotein
Whitt M, Buonocor L, Prehaud C, Rose J. Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoprotein. Virology 1991, 185: 681-688. PMID: 1660200, DOI: 10.1016/0042-6822(91)90539-n.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ViralBase SequenceCell LineCentrifugation, Density GradientCricetinaeFlow CytometryGenetic Complementation TestGlycoproteinsHumansHydrogen-Ion ConcentrationKineticsMacromolecular SubstancesMembrane FusionMembrane GlycoproteinsMiceMolecular Sequence DataPlasmidsRabies virusRecombinant Fusion ProteinsVesicular stomatitis Indiana virusViral Envelope ProteinsViral Fusion ProteinsConceptsVSV G proteinG protein trimersMembrane fusion activityVirus G proteinG proteinsRabies G proteinFusion activityHybrid proteinProtein trimerVesicular stomatitis virus G proteinVirus glycoproteinRabies virus glycoproteinCytoplasmic domainMembrane fusionExtracellular domainHeLa cellsRabies virus G proteinCell surfaceProteinVSV particlesSucrose gradientsVSV infectivityGlycoproteinSpike glycoproteinChemical crosslinkingDissociation and reassociation of oligomeric viral glycoprotein subunits in the endoplasmic reticulum
Zagouras P, Ruusala A, Rose J. Dissociation and reassociation of oligomeric viral glycoprotein subunits in the endoplasmic reticulum. Journal Of Virology 1991, 65: 1976-1984. PMID: 1848313, PMCID: PMC240033, DOI: 10.1128/jvi.65.4.1976-1984.1991.Peer-Reviewed Original ResearchConceptsWild-type ratesEndoplasmic reticulumMutant subunitsG proteinsCell surfaceG protein trimersWild-type subunitsFormation of heterotrimersWild-type moleculeWild-type trimersMutant proteinsRetention signalProtein trimerHeterotrimerSubunitsGlycoprotein subunitsProteinReticulumGlycoprotein formTrimerTransport blockHomotrimersReassociation