Publications

2025

Engineering a genomically recoded organism with one stop codon
Grome M, Nguyen M, Moonan D, Mohler K, Gurara K, Wang S, Hemez C, Stenton B, Cao Y, Radford F, Kornaj M, Patel J, Prome M, Rogulina S, Sozanski D, Tordoff J, Rinehart J, Isaacs F. Engineering a genomically recoded organism with one stop codon. Nature 2025, 639: 512-521. PMID: 39910296, PMCID: PMC11903333, DOI: 10.1038/s41586-024-08501-x.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Structural basis for human NKCC1 inhibition by loop diuretic drugs
Zhao Y, Vidossich P, Forbush B, Ma J, Rinehart J, De Vivo M, Cao E. Structural basis for human NKCC1 inhibition by loop diuretic drugs. The EMBO Journal 2025, 44: 1540-1562. PMID: 39875725, PMCID: PMC11876703, DOI: 10.1038/s44318-025-00368-6.
Peer-Reviewed Original Research
MeSH Keywords and Concepts

2024

Nuclear PKM2 binds pre-mRNA at folded G-quadruplexes and reveals their gene regulatory role
Anastasakis D, Apostolidi M, Garman K, Polash A, Umar M, Meng Q, Scutenaire J, Jarvis J, Wang X, Haase A, Brownell I, Rinehart J, Hafner M. Nuclear PKM2 binds pre-mRNA at folded G-quadruplexes and reveals their gene regulatory role. Molecular Cell 2024, 84: 3775-3789.e6. PMID: 39153475, PMCID: PMC11455610, DOI: 10.1016/j.molcel.2024.07.025.
Peer-Reviewed Original Research
Concepts
Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases
Zhao Y, Schubert H, Blakely A, Forbush B, Smith M, Rinehart J, Cao E. Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases. Nature Communications 2024, 15: 7006. PMID: 39143061, PMCID: PMC11324901, DOI: 10.1038/s41467-024-51381-y.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Elastocapillary effects determine early matrix deformation by glioblastoma cell spheroids
Ang I, Yousafzai M, Yadav V, Mohler K, Rinehart J, Bouklas N, Murrell M. Elastocapillary effects determine early matrix deformation by glioblastoma cell spheroids. APL Bioengineering 2024, 8: 026109. PMID: 38706957, PMCID: PMC11069407, DOI: 10.1063/5.0191765.
Peer-Reviewed Original Research
Concepts

2023

SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces.
Lee S, Yousafzai M, Mohler K, Yadav V, Amiri S, Szuszkiewicz J, Levchenko A, Rinehart J, Murrell M. SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces. Molecular Biology Of The Cell 2023, 34: ar122. PMID: 37672340, PMCID: PMC10846615, DOI: 10.1091/mbc.e23-03-0103.
Peer-Reviewed Original Research
MeSH Keywords
System‐wide optimization of an orthogonal translation system with enhanced biological tolerance
Mohler K, Moen J, Rogulina S, Rinehart J. System‐wide optimization of an orthogonal translation system with enhanced biological tolerance. Molecular Systems Biology 2023, 19: msb202110591. PMID: 37477096, PMCID: PMC10407733, DOI: 10.15252/msb.202110591.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Mapping the in vivo fitness landscape of a tethered ribosome
Radford F, Rinehart J, Isaacs F. Mapping the in vivo fitness landscape of a tethered ribosome. Science Advances 2023, 9: eade8934. PMID: 37115918, PMCID: PMC10146877, DOI: 10.1126/sciadv.ade8934.
Peer-Reviewed Original Research
MeSH Keywords and Concepts

2022

Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine
Moen J, Mohler K, Rogulina S, Shi X, Shen H, Rinehart J. Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine. Nature Communications 2022, 13: 7226. PMID: 36433969, PMCID: PMC9700786, DOI: 10.1038/s41467-022-34980-5.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics
Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Nature Methods 2022, 19: 1371-1375. PMID: 36280721, PMCID: PMC9847208, DOI: 10.1038/s41592-022-01638-5.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Correction: The mechanism of β-N-methylamino-L-alanine inhibition of tRNA aminoacylation and its impact on misincorporation
Han N, Bullwinkle T, Loeb K, Faull K, Mohler K, Rinehart J, Ibba M. Correction: The mechanism of β-N-methylamino-L-alanine inhibition of tRNA aminoacylation and its impact on misincorporation. Journal Of Biological Chemistry 2022, 298: 102544. PMCID: PMC9547288, DOI: 10.1016/j.jbc.2022.102544.
Peer-Reviewed Original Research
Comprehensive Analysis of Metabolic Isozyme Targets in Cancer
Marczyk M, Gunasekharan V, Casadevall D, Qing T, Foldi J, Sehgal R, Shan NL, Blenman KRM, O'Meara TA, Umlauf S, Surovtseva YV, Muthusamy V, Rinehart J, Perry RJ, Kibbey R, Hatzis C, Pusztai L. Comprehensive Analysis of Metabolic Isozyme Targets in Cancer. Cancer Research 2022, 82: 1698-1711. PMID: 35247885, PMCID: PMC10883296, DOI: 10.1158/0008-5472.can-21-3983.
Peer-Reviewed Original Research
MeSH Keywords and Concepts

2021

Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteins
Arranz-Gibert P, Vanderschuren K, Haimovich A, Halder A, Gupta K, Rinehart J, Isaacs FJ. Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteins. Cell Chemical Biology 2021, 29: 1046-1052.e4. PMID: 34965380, PMCID: PMC10173106, DOI: 10.1016/j.chembiol.2021.12.002.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function
Schiapparelli P, Pirman NL, Mohler K, Miranda-Herrera PA, Zarco N, Kilic O, Miller C, Shah SR, Rogulina S, Hungerford W, Abriola L, Hoyer D, Turk BE, Guerrero-Cázares H, Isaacs FJ, Quiñones-Hinojosa A, Levchenko A, Rinehart J. Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function. Cell Reports 2021, 36: 109416. PMID: 34289367, PMCID: PMC8379681, DOI: 10.1016/j.celrep.2021.109416.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Targeting Pyruvate Kinase M2 Phosphorylation Reverses Aggressive Cancer Phenotypes
Apostolidi M, Vathiotis IA, Muthusamy V, Gaule P, Gassaway BM, Rimm DL, Rinehart J. Targeting Pyruvate Kinase M2 Phosphorylation Reverses Aggressive Cancer Phenotypes. Cancer Research 2021, 81: 4346-4359. PMID: 34185676, PMCID: PMC8373815, DOI: 10.1158/0008-5472.can-20-4190.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Deacylated tRNA Accumulation Is a Trigger for Bacterial Antibiotic Persistence Independent of the Stringent Response
Wood WN, Mohler K, Rinehart J, Ibba M. Deacylated tRNA Accumulation Is a Trigger for Bacterial Antibiotic Persistence Independent of the Stringent Response. MBio 2021, 12: 10.1128/mbio.01132-21. PMID: 34126764, PMCID: PMC8262941, DOI: 10.1128/mbio.01132-21.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression
Rosa-Mercado NA, Zimmer JT, Apostolidi M, Rinehart J, Simon MD, Steitz JA. Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression. Molecular Cell 2021, 81: 502-513.e4. PMID: 33400923, PMCID: PMC7867636, DOI: 10.1016/j.molcel.2020.12.002.
Peer-Reviewed Original Research
MeSH Keywords and Concepts

2020

CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA
Schiapparelli P, Meade P, Miranda-Herrera P, Bechtle A, Issacs F, Levchenko A, Rinehart J, Quinones-Hinojosa A. CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA. Neuro-Oncology 2020, 22: ii29-ii29. PMCID: PMC7650317, DOI: 10.1093/neuonc/noaa215.120.
Peer-Reviewed Original Research
Concepts
Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health
Abulizi A, Cardone RL, Stark R, Lewandowski SL, Zhao X, Hillion J, Ma L, Sehgal R, Alves TC, Thomas C, Kung C, Wang B, Siebel S, Andrews ZB, Mason GF, Rinehart J, Merrins MJ, Kibbey RG. Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health. Cell Metabolism 2020, 32: 751-766.e11. PMID: 33147485, PMCID: PMC7679013, DOI: 10.1016/j.cmet.2020.10.006.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
A Membrane-Bound Diacylglycerol Species Induces PKCϵ-Mediated Hepatic Insulin Resistance
Lyu K, Zhang Y, Zhang D, Kahn M, Ter Horst KW, Rodrigues MRS, Gaspar RC, Hirabara SM, Luukkonen PK, Lee S, Bhanot S, Rinehart J, Blume N, Rasch MG, Serlie MJ, Bogan JS, Cline GW, Samuel VT, Shulman GI. A Membrane-Bound Diacylglycerol Species Induces PKCϵ-Mediated Hepatic Insulin Resistance. Cell Metabolism 2020, 32: 654-664.e5. PMID: 32882164, PMCID: PMC7544641, DOI: 10.1016/j.cmet.2020.08.001.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
Metabolic stress promotes stop-codon readthrough and phenotypic heterogeneity
Zhang H, Lyu Z, Fan Y, Evans CR, Barber KW, Banerjee K, Igoshin OA, Rinehart J, Ling J. Metabolic stress promotes stop-codon readthrough and phenotypic heterogeneity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 22167-22172. PMID: 32839318, PMCID: PMC7486758, DOI: 10.1073/pnas.2013543117.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
205-OR: Hepatic Protein Kinase C-e Is Necessary and Sufficient in Mediating Lipid-Induced Hepatic Insulin Resistance
LYU K, ZHANG D, KAHN M, RODRIGUES M, HIRABARA S, LUUKKONEN P, LEE S, BHANOT S, RINEHART J, BLUME N, RASCH M, SERLIE M, BOGAN J, CLINE G, SAMUEL V, SHULMAN G. 205-OR: Hepatic Protein Kinase C-e Is Necessary and Sufficient in Mediating Lipid-Induced Hepatic Insulin Resistance. Diabetes 2020, 69 DOI: 10.2337/db20-205-or.
Peer-Reviewed Original Research
Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane
Wu J, Rowart P, Jouret F, Gassaway BM, Rajendran V, Rinehart J, Caplan MJ. Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane. American Journal Of Physiology - Cell Physiology 2020, 318: c486-c501. PMID: 31913699, PMCID: PMC7099514, DOI: 10.1152/ajpcell.00422.2019.
Peer-Reviewed Original Research
MeSH Keywords and Concepts
The mechanism of β-N-methylamino-l-alanine inhibition of tRNA aminoacylation and its impact on misincorporation
Han N, Bullwinkle T, Loeb K, Faull K, Mohler K, Rinehart J, Ibba M. The mechanism of β-N-methylamino-l-alanine inhibition of tRNA aminoacylation and its impact on misincorporation. Journal Of Biological Chemistry 2020, 295: 1402-1410. DOI: 10.1016/s0021-9258(17)49898-x.
Peer-Reviewed Original Research
Concepts

See All Publications

Publications

2026

Cryptic phosphorylation sites provide new structural and functional insights into the human phosphoproteome

2025

Engineering a genomically recoded organism with one stop codon

Structural basis for human NKCC1 inhibition by loop diuretic drugs

2024

Nuclear PKM2 binds pre-mRNA at folded G-quadruplexes and reveals their gene regulatory role

Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases

Elastocapillary effects determine early matrix deformation by glioblastoma cell spheroids

2023

SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces.

System‐wide optimization of an orthogonal translation system with enhanced biological tolerance

Mapping the in vivo fitness landscape of a tethered ribosome

2022

Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine

A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics

Correction: The mechanism of β-N-methylamino-L-alanine inhibition of tRNA aminoacylation and its impact on misincorporation

Comprehensive Analysis of Metabolic Isozyme Targets in Cancer

2021

Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteins

Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function

Targeting Pyruvate Kinase M2 Phosphorylation Reverses Aggressive Cancer Phenotypes

Deacylated tRNA Accumulation Is a Trigger for Bacterial Antibiotic Persistence Independent of the Stringent Response

Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression

2020

CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA

Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health

A Membrane-Bound Diacylglycerol Species Induces PKCϵ-Mediated Hepatic Insulin Resistance

Metabolic stress promotes stop-codon readthrough and phenotypic heterogeneity

205-OR: Hepatic Protein Kinase C-e Is Necessary and Sufficient in Mediating Lipid-Induced Hepatic Insulin Resistance

Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane

The mechanism of β-N-methylamino-l-alanine inhibition of tRNA aminoacylation and its impact on misincorporation