Publications

Schiapparelli P, Pirman NL, Mohler K, Miranda-Herrera PA, Zarco N, Kilic O,Miller C, Shah SR, Rogulina S, Hungerford W, Abriola L, Hoyer D, Turk BE, Guerrero-Cázares H, Isaacs FJ, Quiñones-Hinojosa A, Levchenko A, Rinehart J. Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function. Cell Reports. 2021 Jul 20;36(3):109416. doi:10.1016/j.celrep.2021.109416. PMID: 34289367.
Apostolidi M, Vathiotis IA, Muthusamy V, Gaule P, Gassaway BM, Rimm DL, Rinehart J. Targeting Pyruvate Kinase M2 phosphorylation reverses aggressive cancer phenotypes. Cancer Res. 2021 Jun 21:canres.4190.2020.CAN-20-4190. PMID: 34185676.
Mohler K, Moen J, Rogulina S, Rinehart J.Principles for Systematic Optimization of an Orthogonal Translation System with Enhanced Biological Tolerance. bioRxiv. 2021. doi: https://doi.org/10.1101/2021.05.20.444985.
Rosa-Mercado NA, Zimmer JT, Apostolidi M, Rinehart J, Simon MD, Steitz JA. Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression. Molecular Cell. 2021 Feb 4;81(3):502-513.e4. PMID: 33400923; PMCID: PMC7867636.
Wood WN, Mohler K, Rinehart J, Ibba M. Deacylated tRNA Accumulation Is a Trigger for Bacterial Antibiotic Persistence Independent of the Stringent Response. mBio. 2021 Jun 29;12(3):e0113221.PMID: 34126764.
Abulizi A, Cardone RL, Stark R, Lewandowski SL, Zhao X, Hillion J, Ma L, Sehgal R, Alves TC, Thomas C, Kung C, Wang B, Siebel S, Andrews ZB, Mason GF, Rinehart J, Merrins MJ, Kibbey RG. Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health. Cell Metabolism. 2020 Nov 3;32(5):751-766.e11. PMID:33147485; PMCID: PMC7679013.
Lyu K, Zhang Y, Zhang D, Kahn M, Ter Horst KW, Rodrigues MRS, Gaspar RC, Hirabara SM, Luukkonen PK, Lee S, Bhanot S, Rinehart J, Blume N, Rasch MG, Serlie MJ, Bogan JS, Cline GW, Samuel VT, Shulman GI. A Membrane-Bound Diacylglycerol Species Induces PKC?-Mediated Hepatic Insulin Resistance. Cell Metabolism. 2020 Oct 6;32(4):654-664.e5. PMID: 32882164; PMCID:PMC7544641.
Zhang H, Lyu Z, Fan Y, Evans CR, Barber KW, Banerjee K, Igoshin OA, Rinehart J, Ling J. Metabolic stress promotes stop-codon readthrough and phenotypic heterogeneity. PNAS. 2020 Sep 8;117(36):22167-22172. PMID: 32839318; PMCID: PMC7486758.
Wu J, Rowart P, Jouret F, Gassaway BM, Rajendran V, Rinehart J, Caplan MJ. Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane. Am J Physiol Cell Physiol. 2020 Mar 1;318(3):C486-C501. PMID: 31913699; PMCID:PMC7099514.
Han NC, Bullwinkle TJ, Loeb KF, Faull KF, Mohler K, Rinehart J, Ibba M. The mechanism of ß-N-methylamino-l-alanine inhibition of tRNA aminoacylation and its impact on misincorporation. J Biol Chem. 2020 Jan 31;295(5):1402-1410. PMID: 31862734; PMCID:PMC6996898.
Kelly P, Backes N, Mohler K, Buser C, Kavoor A, Rinehart J, Phillips G, Ibba M. Alanyl-tRNA Synthetase Quality Control Prevents Global Dysregulation of the Escherichia coli Proteome. mBio. 2019 Dec 17;10(6):e02921-19. doi:10.1128/mBio.02921-19. PMID: 31848288; PMCID: PMC6918089.
Gassaway, Brandon M., Rebecca L. Cardone, Anil K. Padyana, Max C. Petersen, Evan T. Judd, Sebastian Hayes, Shuilong Tong, Karl W. Barber, Maria Apostolidi, Abudukadier Abulizi, Joshua B. Sheetz, Kshitiz, Hans R. Aerni, Stefan Gross, Charles Kung, Varman T. Samuel, Gerald I. Shulman, Richard G. Kibbey, Jesse Rinehart.Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production Cell Reports Vol. 29, Issue 11, P3394-3404.E9, December 10, 2019.
Ma NJ, Hemez CF, Barber KW, Rinehart J, Isaacs FJ. Organisms with alternative genetic codes resolve unassigned codons via mistranslation and ribosomal rescue. eLife. 2018 Oct 30;7. pii: e34878. doi: 10.7554/eLife.34878.
Gassaway, Brandon M., M.C. Petersen, Y.V. Surovtseva, K.W. Barber, J.B. Sheetz, H.R. Aerni, J. Merkel, V.T. Samuel, G.I. Shulman, J. Rinehart. PKCe contributes to lipid-induced insulin resistance through crosstalk with p70S6K and through novel regulators of insulin signaling. Proc. Natl. Acad. Sci. USA. September 18, 2018 115 (38) E8996-E9005; published ahead of print September 4, 2018.
Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, and Rinehart J. Encoding the human serine phosphoproteome in an engineered bacterial system.Nature Biotechnology. 2018. Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11.
Barber KW, Miller CJ, Jun JW, Lou HJ, Turk BE, Rinehart J.Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Biochemistry. 2018 Jun 19. doi: 10.1021/acs.biochem.8b00410
Barber KW and Rinehart J. The ABCs of PTMs.Nat Chem Biol. 2018 Feb 14;14(3):188-192.
Fan Y, Evans CR, Barber KW, Banerjee K, Weiss KJ, Margolin W, Igoshin OA, Rinehart J, Ling J. Heterogeneity of Stop Codon Readthrough in Single Bacterial Cells and Implications for Population Fitness. Mol Cell. 2017 Aug 1. pii: S1097-2765(17)30504-X.
Barber KW and Rinehart J. Expression of Recombinant Phosphoproteins for Signal Transduction Studies. Methods Mol. Biol. 2017;1636:71-78.
Castañeda-Bueno M, Arroyo JP, Zhang J, Puthumana J, Yarborough O, Shibata S, Rojas-Vega L, Gamba G, Rinehart J, Lifton RP. Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4.Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E879-E886.
Park HB, Perez CE, Barber KW, Rinehart J, Crawford JM. Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster. eLife. March 15, 2017;10.7554/eLife.25229.
Padovano V, Kuo IY, Stavola LK, Aerni HR, Flaherty BJ, Chapin HC, Ma M, Somlo S, Boletta A, Ehrlich BE, Rinehart J, Caplan MJ. The polycystins are modulated by cellular oxygen-sensing pathways and regulate mitochondrial function.Mol Biol Cell. 2017 Jan 15;28(2):261-269.
Mohler K, Aerni HR, Gassaway B, Ling J, Ibba M, Rinehart J. MS-READ: Quantitative measurement of amino acid incorporation. Biochim Biophys Acta. 2017 Jan 24. pii: S0304-4165(17)30033-8.
Mohler K, Mann R, Bullwinkle TJ, Hopkins K, Hwang L, Reynolds NM, Gassaway B, Aerni HR, Rinehart J, Polymenis M, Faull K, Ibba M. Editing of misaminoacylated tRNA controls the sensitivity of amino acid stress responses in Saccharomyces cerevisiae.Nucleic Acids Res. 2017 Feb 7. Apr 20;45(7):3985-3996.
Ferdaus MZ, Barber KW, López-Cayuqueo KI, Terker AS, Argaiz ER, Gassaway BM, Chambrey R, Gamba G, Rinehart J, and McCormick JA. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule.J Physiol. 2016 Sep 1;594(17):4945-66.
Petersen MC, Madiraju AK, Gassaway BM, Marcel M, Nasiri AR, Butrico G, Marcucci MJ, Zhang D, Abulizi A, Zhang XM, Philbrick W, Hubbard SR, Jurczak MJ, Samuel VT, Rinehart J, Shulman GI. Insulin receptor Thr1160 phosphorylation mediates lipid-induced hepatic insulin resistance.J Clin Invest. 2016 11:4361-4371.
Amiram M, Haimovich AD, Fan C, Wang Y-S, Aerni H-R, Ioanna N, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll D,Rinehart J, Isaacs FJ. Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nature Biotechnology Published online 16 November 2015. doi:10.1038/nbt.3372.
Pirman NL, Barber KW, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, andRinehart J. A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nature Communications. 2015. 6, 8130.
Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ, Rinehart J, and Jewett MC. Robust Production of Recombinant Phosphoproteins Using Cell-Free Protein Synthesis. Nature Communications 2015. 6, 8168
Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW. The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a Program of OPTN/NDP52 Recruitment and TBK1 Activation to Promote Mitophagy. Molecular Cell. 2015. Sep 9. pii: S1097-2765(15)00662-0. doi: 10.1016/j.molcel.2015.08.016. [Epub ahead of print].
Ordureau A, Heo J,Duda DM,Paulo JA, Olszewski JL, Yanishevski D, Rinehart J, Schulman BA, and J. Wade Harper JW. Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy PNAS 2015 ; published ahead of print May 12, 2015, doi:10.1073/pnas.1506593112
Rovner AJ, Haimovich AD, Katz SR, Li Z, Grome MW, Gassaway BM, Amiram, Patel JR, Gallagher RR, Rinehart J, and Isaacs F. Recoded organisms engineered to depend on synthetic amino acids. Nature 2015. Feb 5;518(7537):89-93.
Aerni HR, Shifman MA, Rogulina S, O’Donoghue P, Rinehart J. Revealing the amino acid composition of proteins within an expanded genetic code. Nucleic Acids Research. 2015 Jan;43(2):e8.
Lin DH, Yue P, Yarborough O 3rd, Scholl UI, Giebisch G, Lifton RP, Rinehart J, Wang WH. Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK). Proc Natl Acad Sci USA. 2015 Mar 24.
O’Donoghue P, Prat L, Kucklick M, Schäfer JG, Riedel K, Rinehart J, Söll D, Heinemann IU. Reducing the genetic code induces massive rearrangement of the proteome. PNAS 2014 ; published ahead of print Nov. 17, 2014, doi:10.1073/pnas.1420193111
Colangelo CM, Ivosev G, Chung L, Abbott T, Shifman M, Sakaue F, Cox D, Kitchen RR, Burton L, Tate SA, Gulcicek E, Bonner R, Rinehart J, Nairn AC, Williams KR.Development of a highly automated and multiplexed targeted proteome pipeline and assay for 112 rat brain synaptic proteins. Proteomics 2014, DOI: 10.1002/pmic.201400353
Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L.Designed Phosphoprotein Recognition in Escherichia coli. ACS Chem Biol. 2014 Oct 6. [Epub ahead of print]
Steinfeld JB, Aerni HR, Rogulina S, Liu Y, Rinehart J. Cellular Amino Acid Pools Containing Phosphoserine, Phosphothreonine, and Phosphotyrosine. ACS Chem Biol. article ASAP online 2014 Mar 27.
Marc J. Lajoie, Alexis J. Rovner, Daniel B. Goodman, Hans-Rudolf Aerni, Adrian D. Haimovich, Gleb Kuznetsov, Jaron A. Mercer, Harris H. Wang, Peter A. Carr, Joshua A. Mosberg, Nadin Rohland, Peter G. Schultz, Joseph M. Jacobson, Jesse Rinehart, George M. Church, and Farren J. Isaacs.Genomically Recoded Organisms Impart New Biological Functions. Science. 2013 Oct 18;342(6156):357-60.
Shibata S, Rinehart J, Zhang J, Moeckel G, Castañeda-Bueno M, Stiegler A, Boggon T, Gamba G, Lifton R. Mineralocorticoid Receptor Phosphorylation Regulates Ligand Binding and Renal Response to Volume Depletion and Hyperkalemia. Cell Metabolism. 2013 Nov 5;18(5):609-10.
Zhang C, Wang L, Thomas S, Wang K, Lin DH, Rinehart J, Wang WH. Src Family Protein Tyrosine Kinase Regulates the Basolateral K Channel in the Distal Convoluted Tubule (DCT) by Phosphorylation of KCNJ10 Protein. J Biol Chem. 2013 Sep 6;288(36):26135-46.PMID: 23873931
Radha Krishnakumar, Laure Prat, Hans-Rudolf Aerni, Jiqiang Ling, Chuck Merryman, John I. Glass, Jesse Rinehart, and Dieter Söll. Transfer RNA Misidentification Scrambles Sense Codon Recoding. Chembiochem. 2013 Sep 2. doi: 10.1002/cbic.201300444.
Prat L, Heinemann IU, Aerni HR, Rinehart J, O’Donoghue P, Söll D. Carbon source-dependent expansion of the genetic code in bacteria. PNAS. 2012 Dec 18;109(51):21070-5. PMID: 23185002
Ling J, Cho C, Guo LT, Aerni HR, Rinehart J, Söll D. Protein aggregation caused by aminoglycoside action is prevented by a hydrogen peroxide scavenger. Mol Cell. 2012 Dec 14;48(5):713-22. PMID: 23122414.
*Heinemann IU, *Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Söll D, Isaacs FJ, Rinehart J. Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. FEBS Lett. 2012 Oct 19;587(20):3716-22. Epub 2012 Sep 13. PMID: 22982858.
Zarychanski R, Schulz VP, Houston BL, Maksimova Y, Houston DS, Smith B, Rinehart J, Gallagher PG.Mutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis. Blood. 2012 Aug 30;120(9):1908-15. PMID: 22529292
Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren C J, Rinehart J, Söll D. Expanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26; 333:1151-1154. PMID: 21868676
*Rinehart J, *Vázquez N, Kahle KT, Hodson CA, Ring AM, Gulcicek EE, Louvi A, Bobadilla NA, Gamba G, Lifton RP. WNK2 Kinase Is a Novel Regulator of Essential Neuronal Cation-Chloride Cotransporters. J Biol Chem. 2011 Aug 26;286(34):30171-80. PMID: 21733846
Zhang L, Jouret F, Rinehart J, Sfakianos J, Mellman I, Lifton RP, Young LH, Caplan MJ.AMP-activated protein kinase (AMPK) activation and glycogen synthase kinase 3beta (GSK-3beta) inhibition induce Ca2+-independent deposition of tight junction components at the plasma membrane. J Bio Chem. 2011 May 13;286:16879-16890. PMID: 21383016
Monette MY, Rinehart J, Lifton RP, Forbush B. Rare mutations in the human Na-K-Cl cotransporter (NKCC2) associated with lower blood pressure exhibit impaired processing and transport function. Am J Phys Renal Phys. 2011 April; 300:F840-847. PMID: 21209010
Rinehart J, Gulcicek, EE, Joiner, CH, Lifton, RP, and Gallagher, PG. Determinants of erythrocyte hydration. Current Opinion in Hematology. 2010 May;17(3):191-197. PMID: 20182354
Rinehart J, Maksimova YD, Tanis JE, Stone KL, Hodson CA, Zhang J, Risinger M, Pan W, Wu D, Colangelo CM, Forbush B, Joiner CH, Gulcicek EE, Gallagher PG, Lifton RP. Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity. Cell. 2009 Aug 7;138(3):525-36. PMID: 19665974
Kahle KT, Rinehart J, Giebisch G, Gamba G, Hebert SC, Lifton RP. A novel protein kinase signaling pathway essential for blood pressure regulation in humans. Trends Endocrinol Metab. 2008 Apr;19(3):91-5. PMID: 18280177
Ring AM, Cheng SX, Leng Q, Kahle KT, Rinehart J, Lalioti MD, Volkman HM, Wilson FH, Hebert SC, and Lifton RP. WNK4 regulates activity of the epithelial Na+ channel in vitro and in vivo. Proc. Natl. Acad. Sci. USA. 2007 Mar 6;104(10):4020-4. PMID: 17360470
Ring AM, Leng Q, Rinehart J, Wilson FH, Kahle, KT, Hebert, SC, and Lifton RP. An SGK1 site in WNK4 regulates ENaC and ROMK activity: Implications for aldosterone signaling and K+ homeostasis. Proc. Natl. Acad. Sci. USA. 2007 Mar 6;104(10): 4025-9. PMID: 17360471
Kahle KT, Rinehart J, Ring A, Gimenez I, Gamba G, Hebert SC, Lifton RP.WNK protein kinases modulate cellular Cl- flux by altering the phosphorylation state of the Na-K-Cl and K-Cl cotransporters. Physiology. 2006 Oct;21:326-35. PMID: 16990453
Leng Q, Kahle KT, Rinehart J, MacGregor GG, Wilson FH, Canessa CM, Lifton RP, Hebert SC. WNK3, a kinase related to genes mutated in hereditary hypertension with hyperkalaemia, regulates the K+ channel ROMK1 (Kir1.1) J. Physiol. 2006 Mar 1;571(Pt 2):275-86. PMID: 16357011
de Los Heros P, Kahle KT, Rinehart J, Bobadilla NA, Vazquez N, San Cristobal P, Mount DB, Lifton RP, Hebert SC, Gamba G. WNK3 bypasses the tonicity requirement for K-Cl cotransporter activation via a phosphatase-dependent pathway. Proc. Natl. Acad. Sci. USA. 2006 Feb 7;103(6):1976-81. PMID: 16446421
Rinehart J, Kahle KT, de Los Heros P, Vazquez N, Meade P, Wilson FH, Hebert SC, Gimenez I, Gamba G, Lifton RP. WNK3 kinase is a positive regulator of NKCC2 and NCC, renal cation-Cl-cotransporters required for normal blood pressure homeostasis. Proc. Natl. Acad. Sci. USA. 2005 Nov 15;102(46):16777-82. PMID: 16275913
Kahle KT, Rinehart J, de Los Heros P, Louvi A, Meade P, Vazquez N, Hebert SC, Gamba G, Gimenez I, Lifton RP. WNK3 modulates transport of Cl- in and out of cells: implications for control of cell volume and neuronal excitability. Proc. Natl. Acad. Sci. USA. 2005 Nov 15;102(46):16783-8. PMID: 16275911
Rubio MA, Rinehart J, Krett B, Duvezin-Caubet S, Reichert A, Söll D, and Alfonzo JD. Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import. Proc. Natl. Acad. Sci. USA. 2008 Jul 8;105(27):9186-91. PMID: 18587046
Rinehart J, Krett B, Rubio MA, Alfonzo JD, Söll D. Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion.Genes & Development. 2005 Mar 1;19(5):583-92. PMID: 15706032
Rinehart J, Horn EK, Wei D, Söll D, Schneider A. Non-canonical eukaryotic glutaminyl- and glutamyl-tRNA synthetases form mitochondrial aminoacyl-tRNA in Trypanosoma brucei. J. Biol. Chem. 2004 Jan 9; 279(2):1161-6. PMID: 14563839
Rosas-Sandoval G, Ambrogelly A, Rinehart J, Wei D, Cruz-Vera LR, Graham DE, Stetter KO, Guarneros G, Söll D. Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast. Proc. Natl. Acad. Sci. USA. 2002 24 Dec;99:16707-16712. PMID: 12475929