Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethality
Shipitsin M, Small C, Giladi E, Siddiqui S, Choudhury S, Hussain S, Huang YE, Chang H, Rimm DL, Berman DM, Nifong TP, Blume-Jensen P. Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethality. Proteome Science 2014, 12: 40. PMID: 25075204, PMCID: PMC4114438, DOI: 10.1186/1477-5956-12-40.Peer-Reviewed Original ResearchProtein levelsPost-translational modificationsProtein-based approachGene-based approachesIntact tissue specimensProtein activityProtein signaturesHuman prostate cancerMolecular informationPredictive protein biomarkersProtein biomarker levelsPhospho-PRAS40Phospho-S6Prostate cancer lethalityTissue lysisSPP1PTENIntact tissueSMAD4Cancer lethalityPhenotypeCCND1Morphological featuresProtein biomarkersFunctional activity