2011
A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion
Krishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion. Nature Structural & Molecular Biology 2011, 18: 934-940. PMID: 21785412, PMCID: PMC3668341, DOI: 10.1038/nsmb.2103.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAmino Acid SequenceAnimalsBinding SitesCrystallography, X-RayHumansMembrane FusionModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsProtein Structure, TertiaryRatsSynaptosomal-Associated Protein 25SynaptotagminsSyntaxin 1Vesicle-Associated Membrane Protein 2
2010
Structure and function of the polymerase core of TRAMP, a RNA surveillance complex
Hamill S, Wolin SL, Reinisch KM. Structure and function of the polymerase core of TRAMP, a RNA surveillance complex. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 15045-15050. PMID: 20696927, PMCID: PMC2930566, DOI: 10.1073/pnas.1003505107.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid SequenceBase SequenceBinding SitesCrystallography, X-RayDNA-Directed DNA PolymeraseModels, MolecularMolecular Sequence DataMultiprotein ComplexesProtein Interaction Domains and MotifsRecombinant ProteinsRNA, FungalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidStatic ElectricitySubstrate SpecificityConceptsZinc knuckle motifHigher eukaryotesSubstrate recognitionRNA polymeraseCentral domainInitial substrate recognitionRNA 3' endsTRAMP complexRNA surveillanceZinc knuckleCharacterized enzymesAberrant RNAsSurveillance complexPolymerase coreRNA bindingAir2pNucleotidyl transferaseTrf4pN-terminusEukaryotesInteraction surfacePolymeraseMotifNucleic acidsComplexes
2005
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif
Dong G, Hutagalung AH, Fu C, Novick P, Reinisch KM. The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nature Structural & Molecular Biology 2005, 12: 1094-1100. PMID: 16249794, DOI: 10.1038/nsmb1017.Peer-Reviewed Original ResearchStructural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity
Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM. Structural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity. Cell 2005, 121: 529-539. PMID: 15907467, PMCID: PMC1769319, DOI: 10.1016/j.cell.2005.03.009.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsBinding SitesCells, CulturedCrystallography, X-RayInsectaModels, MolecularMolecular Sequence DataNucleic Acid ConformationProtein BindingProtein Structure, TertiaryRibonucleoproteinsRNARNA Processing, Post-TranscriptionalRNA, Small CytoplasmicRNA, Small NuclearRNA-Binding ProteinsXenopus laevis
2004
Structure of the La motif: a winged helix domain mediates RNA binding via a conserved aromatic patch
Dong G, Chakshusmathi G, Wolin SL, Reinisch KM. Structure of the La motif: a winged helix domain mediates RNA binding via a conserved aromatic patch. The EMBO Journal 2004, 23: 1000-1007. PMID: 14976553, PMCID: PMC380972, DOI: 10.1038/sj.emboj.7600115.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsAutoantigensConserved SequenceCrystallography, X-RayHelix-Turn-Helix MotifsHydroxylationModels, MolecularMolecular Sequence DataMutationPhosphatesProtein BindingProtein Structure, TertiaryRibonucleoproteinsRNASequence AlignmentSubstrate SpecificityTrypanosoma brucei bruceiConceptsLa motifLa proteinRNA polymerase III transcriptsFirst structural insightsRNA-binding proteinPolymerase III transcriptsHelix domainNuclear phosphoproteinRNA substratesMutagenesis experimentsStructural insightsConserved regionsHigh-affinity bindingAromatic patchHelix architectureProteinSurface residuesMotifRNAUridylateCritical roleTranscriptsPhosphoproteinExonucleaseFolding