2019
Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry
Ramaglia V, Sheikh-Mohamed S, Legg K, Park C, Rojas OL, Zandee S, Fu F, Ornatsky O, Swanson EC, Pitt D, Prat A, McKee TD, Gommerman JL. Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry. ELife 2019, 8: e48051. PMID: 31368890, PMCID: PMC6707785, DOI: 10.7554/elife.48051.Peer-Reviewed Original ResearchConceptsMultiple sclerosisMS disease activityT-cell phenotypeMass cytometryTypes of lymphocytesMultiple sclerosis lesionsNatalizumab cessationDisease activityMS patientsInflammatory lesionsImmune cellsSpinal cordLesion morphometryMS lesionsB cellsLesion typeSclerosis lesionsLesionsBlood vesselsCell phenotypeFunctional stateCytometryCellular contentCell-cell interactionsPhenotype
2016
Iron in Multiple Sclerosis and Its Noninvasive Imaging with Quantitative Susceptibility Mapping
Stüber C, Pitt D, Wang Y. Iron in Multiple Sclerosis and Its Noninvasive Imaging with Quantitative Susceptibility Mapping. International Journal Of Molecular Sciences 2016, 17: 100. PMID: 26784172, PMCID: PMC4730342, DOI: 10.3390/ijms17010100.Peer-Reviewed Original ResearchConceptsMultiple sclerosisMagnetic resonance imagingBrain tissueQuantitative susceptibility mappingMS brain tissueAdvanced MRI methodsMS patientsChronic inflammationImmunohistochemical investigationBrain ironMyeloid cellsResonance imagingNon-invasive studyHistological studyRole of ironOxidative stressNoninvasive imagingSclerosisInflammationCellular distributionMRI methodsNeurodegenerationTissueImagingPatients
2015
Analysis of miRNA in Normal Appearing White Matter to Identify Altered CNS Pathways in Multiple Sclerosis
Guerau-de-Arellano M, Liu Y, Meisen WH, Pitt D, Racke MK, Lovett-Racke AE. Analysis of miRNA in Normal Appearing White Matter to Identify Altered CNS Pathways in Multiple Sclerosis. Journal Of Autoimmune Disorders 2015, 1 PMID: 26894232, PMCID: PMC4755487, DOI: 10.21767/2471-8153.100006.Peer-Reviewed Original ResearchCNS pathwaysMS patientsNormal Appearing White MatterBlood-brain barrierMultiple sclerosis susceptibilityPatients' CNSCNS inflammationMultiple sclerosisNeuroprotective mechanismsPost-transcriptional dysregulationControl subjectsUnderlying dysregulationImmune systemWhite matterCNSInflammationMiR-191Target predicationNAWMMiRNA profiling studiesPatientsMRNA analysisMAPK pathwayGenetic contributorsPathway analysis
2013
Quantitative Susceptibility Mapping of Multiple Sclerosis Lesions at Various Ages
Chen W, Gauthier SA, Gupta A, Comunale J, Liu T, Wang S, Pei M, Pitt D, Wang Y. Quantitative Susceptibility Mapping of Multiple Sclerosis Lesions at Various Ages. Radiology 2013, 271: 183-92. PMID: 24475808, PMCID: PMC4263629, DOI: 10.1148/radiol.13130353.Peer-Reviewed Original ResearchConceptsNormal-appearing white matterMS lesionsMultiple sclerosis lesionsEnhanced lesionsQuantitative susceptibility mappingMR examinationsSclerosis lesionsConventional magnetic resonance imagingMagnetic resonance imagingT2-weighted imagesMS patientsPathophysiologic featuresCerebrospinal fluidOnline supplemental materialWhite matterLesionsQSM imagesResonance imagingPatientsMR imagingThree-dimensional gradient-echo sequenceGradient echo sequenceMonthsBonferroni correctionAgeIron Is a Sensitive Biomarker for Inflammation in Multiple Sclerosis Lesions
Mehta V, Pei W, Yang G, Li S, Swamy E, Boster A, Schmalbrock P, Pitt D. Iron Is a Sensitive Biomarker for Inflammation in Multiple Sclerosis Lesions. PLOS ONE 2013, 8: e57573. PMID: 23516409, PMCID: PMC3597727, DOI: 10.1371/journal.pone.0057573.Peer-Reviewed Original ResearchConceptsMyelin-laden macrophagesMS patientsSecondary progressive MS patientsProgressive MS patientsSecondary progressive MSMultiple sclerosis patientsMacrophages/microgliaWhite matter lesionsHuman macrophage culturesIron-containing macrophagesMultiple sclerosis lesionsImportant clinical informationHuman cultured macrophagesActive relapsingDemyelinating lesionsDisease-relevant processesProgressive MSDemyelinated lesionsSclerosis patientsMultiple sclerosisMatter lesionsM1 polarizationImmunohistochemical examinationMyelin phagocytosisProinflammatory polarization
2011
T1 and proton density at 7 T in patients with multiple sclerosis: an initial study
Bluestein KT, Pitt D, Knopp MV, Schmalbrock P. T1 and proton density at 7 T in patients with multiple sclerosis: an initial study. Magnetic Resonance Imaging 2011, 30: 19-25. PMID: 21937183, PMCID: PMC3375320, DOI: 10.1016/j.mri.2011.07.018.Peer-Reviewed Original ResearchConceptsCortical lesionsMultiple sclerosisWhite matterGray matterHealthy control subjectsWhite matter lesionsNormal-appearing tissueMS patientsMagnetic resonance sequencesMatter lesionsControl subjectsCerebrospinal fluidMS researchLesionsPatientsMR imagingUltrahigh-field MR imagingField MR imagingSclerosisProton densityResonance sequencesTissue responseTurbo fieldMagnetic resonanceInitial studyWhat happens when natalizumab therapy is stopped?
Schaaf SM, Pitt D, Racke MK. What happens when natalizumab therapy is stopped? Expert Review Of Neurotherapeutics 2011, 11: 1247-1250. PMID: 21864070, DOI: 10.1586/ern.11.109.Peer-Reviewed Original ResearchMS disease activityDisease activityActive diseaseNatalizumab cessationTreatment interruptionMultiple sclerosisBaseline levelsSimilar disease activityProgressive multifocal leukoencephalopathyNatalizumab exposureNatalizumab therapyNatalizumab treatmentMultifocal leukoencephalopathyMS patientsMRI measuresMS treatmentEvidence of reboundDiseaseElimination kineticsNatalizumabIntegrin antagonistsTreatmentPatientsCessationLeukoencephalopathy