2012
Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data
Auburn S, Campino S, Miotto O, Djimde A, Zongo I, Manske M, Maslen G, Mangano V, Alcock D, MacInnis B, Rockett K, Clark T, Doumbo O, Ouédraogo J, Kwiatkowski D. Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data. PLOS ONE 2012, 7: e32891. PMID: 22393456, PMCID: PMC3290604, DOI: 10.1371/journal.pone.0032891.Peer-Reviewed Original ResearchConceptsHost diversityIllumina GA platformMultiplicity of infectionGenome-wide datasetsPopulation-level diversitySingle-molecule sequencing technologiesNext-generation sequence dataPlasmodium falciparum diversityMsp-1 locusGenetic divergenceSequence dataSequencing technologiesNumber of clonesLevel diversityNovel insightsDiversityP. falciparum fieldLociMSP-1PCRRelative proportionsNew statistical approachSNPsClonesReduced sensitivity
2003
Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection.
Kengne P, Ouédraogo J, Zampan H, Veas F, Guiguemdé T. Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection. Parassitologia 2003, 45: 27-31. PMID: 15270541.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAntimalarialsDNA Mutational AnalysisDNA, ProtozoanDrug ResistanceFolic Acid AntagonistsHumansMalaria, FalciparumMutation, MissenseParasitemiaPlasmodium falciparumPoint MutationPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthProguanilProtozoan ProteinsPyrimethamineTetrahydrofolate DehydrogenaseConceptsMutation-specific PCRCycloguanil resistanceBlood samplesResistance isolatesResistance mutationsPlasmodium falciparumMixed infectionsChemosensitivity analysisBloodWhole bloodParasite lysateRapid DNA extractionPCR productsRestriction endonuclease digestionPCRAmplified productsDry filter paperPCR technologyPoint mutationsEndonuclease digestionUndigested samplesPatientsInfectionPyrimethamineMutations