Publications

The DNA Methylcytosine Dioxygenase Tet2 Sustains Immunosuppressive Function of Tumor-Infiltrating Myeloid Cells to Promote Melanoma Progression. Wen Pan, Shu Zhu, Kun Qu, Katrina Meeth, Jijun Cheng, Hongdi Ma, Yan Liao, Xizhi Wen, Christine Roden, Zuzana Tobiasova, Zheng Wei, Jun Zhao, Jun Liu, Ji Zheng, Bo Guo, Marcus Bosenberg, Richard A.Flavell, Jun Lu. The DNA Methylcytosine Dioxygenase Tet2 Sustains Immunosuppressive Function of Tumor-Infiltrating Myeloid Cells to Promote Melanoma Progression. Immunity, 47(2):284-297, 2017

Novel Determinants of Mammalian Primary microRNA Processing Revealed by Systematic Evaluation of Hairpin-containing Transcripts and Human Genetic Variation. Christine Roden, Jonathan Gaillard, Shaveta Kanoria, William Rennie, Syndi Barish, Jijun Cheng, Wen Pan, Jun Liu, Chris Cotsapas, Ye Ding and Jun Lu. Novel Determinants of Mammalian Primary microRNA Processing Revealed by Systematic Evaluation of Hairpin-containing Transcripts and Human Genetic Variation. Genome Research, 27(3):374-384, 2017

A Molecular Chipper Technology for CRISPR sgRNA Library Generation and Functional Mapping of Noncoding Regions. Jijun Cheng, Christine Roden, Wen Pan, Shu Zhu, Anna Baccei, Xinghua Pan, Tingting Jiang, Yuval Kluger, Sherman Weissman, Shangqin Guo, Richard Flavell, Ye Ding and Jun Lu. A Molecular Chipper Technology for CRISPR sgRNA Library Generation and Functional Mapping of Noncoding Regions. Nature Communications, 7:11178, 2016

Non-stochastic reprogramming from a privileged somatic cell state. Shangqin Guo*, Xiaoyuan Zi, Vince Schultz, Mei Zhong, Jijun Cheng, Sebastian Koochaki, Cynthia M. Megyola, Xinghua Pan, Kartoosh Heydari, Sherman M. Weissman, Patrick G. Gallagher, Diane S. Krause, Rong Fan, and Jun Lu. Non-stochastic reprogramming from a privileged somatic cell state. Cell, 156(4): 649-62, 2014.

An Extensive Network of TET2-Targeting microRNAs Regulates Malignant Hematopoiesis. Jijun Cheng, Shangqin Guo, Suning Chen, Stephen Mastriano, Chaochun Liu, Ana D’Alessio, Eriona Hysolli, Yanwen Guo, Hong Yao, Cynthia Megyola, Dan Li, Jun Liu, Wen Pan, Christine Roden, Kartoosh Heydari, Jianjun Chen, In-Hyun Park, Ye Ding, Yi Zhang, and Jun Lu. An Extensive Network of TET2-Targeting microRNAs Regulates Malignant Hematopoiesis. Cell Reports, 5:1-11, 2013

An In Vivo Functional Screen Uncovers miR-150-Mediated Regulation of Hematopoietic Injury Response. Brian D. Adams, Shangqin Guo, Haitao Bai, Yanwen Guo, Cynthia Megyola, Jijun Cheng, Kartoosh Heydari, Changchun Xiao, E. Premkumar Reddy, and Jun Lu. An In Vivo Functional Screen Uncovers miR-150-Mediated Regulation of Hematopoietic Injury Response. Cell Reports, 2(4):1048-1060, 2012

Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms. Shangqin Guo, Haitao Bai, Cynthia Megyola, Stephanie Halene, Diane S. Krause, David T. Scadden and Jun Lu. Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms. PNAS, 2012

MicroRNA expression profiles classify human cancers. Jun Lu, Gad Getz, Eric A. Miska, Ezequiel A. Alvarez-Saavedra, Justin Lamb, David Peck, Alejandro Sweet-Cordero, Benjamin L. Ebert, Raymond H. Mak, Adolfo A. Ferrando, James R. Downing, Tyler Jacks, H. Robert Horvitz and Todd R. Golub. MicroRNA expression profiles classify human cancers. Nature, 435(7043):834-8, 2005

Scadden and Todd R. Golub. microRNA-mediated control of of cell fate in megakaryocyte-erythrocyte progenitors. Jun Lu, Shangqin Guo, Benjamin L. Ebert, Hao Zhang, Xiao Peng, Jocelyn Bosco, Jennifer Pretz, Rita Schlanger, Judy H. Wang, Raymond H. Mak, David M. Dombkowski, Frederic I. Preffer, David T. Scadden and Todd R. Golub. microRNA-mediated control of of cell fate in megakaryocyte-erythrocyte progenitors. Developmental Cell, 14(6):843-53, 2008

Distinct microRNA expression profiles in acute myeloid leukemia with common translocations. Zejuan Li, Jun Lu (co-first author), Miao Sun, Shuangli Mi, Hao Zhang, Roger T. Luo, Zhijian Qian, Mary Beth Neilly, Yungui Wang, Jie Jin, Yanming Zhang, Stefan K. Bohlander, Richard Larson, Michelle Le Beau, Michael J. Thirman, Todd R. Golub, Janet D. Rowley, and Jianjun Chen. MicroRNA expression profiles distinguish common translocations in AML. PNAS, 105(40): 15535-40, 2008

MicroRNA expression signatures accurately discriminate acute lymphoblastic leukemia from acute myeloid leukemia. Shuangli Mi, Jun Lu (co-first author), Miao Sun, Zejuan Li, Hao Zhang, Mary Beth Neilly, Yungui Wang, Zhijian Qian, Jie Jin, Yanming Zhang, Stefan K. Bohlander, Michelle M. LeBeau, Richard A. Larson, Todd R. Golub, Janet D. Rowley and Jianjun Chen. MicroRNA expression signatures accurately discriminate acute lymphoblastic leukemia from acute myeloid leukemia. PNAS, 104(50):19971-6, 2007

MicroRNA miR-125a controls hematopoietic stem cell number. Guo S, Lu J (co-first), Schlanger R, Zhang H, Wang JY, Fox MC, Purton LE, Fleming HH, Cobb B, Merkenschlager M, Golub TR, Scadden DT, MicroRNA miR-125a controls hematopoietic stem cell number, PNAS, 107(32):14229-34, 2010

Systematic discovery of regulatory motifs in human promoters and 3’ UTRs by comparison of several mammals. Xiaohui Xie, Jun Lu, E. J. Kulbokas, Todd R. Golub, Vamsi Mootha, Kerstin Lindblad-Toh, Eric S. Lander and Manolis Kellis. Systematic discovery of regulatory motifs in human promoters and 3’ UTRs by comparison of several mammals. Nature, 434 (7031):338-45, 2005

Full List of PubMed Publications

Wang N, Cheng J, Fan R, Lu J: Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate. Analyst. 2017 Aug 21. PMID: 28765841

Pan W, Zhu S, Qu K, Meeth K, Cheng J, He K, Ma H, Liao Y, Wen X, Roden C, Tobiasova Z, Wei Z, Zhao J, Liu J, Zheng J, Guo B, Khan SA, Bosenberg M, Flavell RA, Lu J: The DNA Methylcytosine Dioxygenase Tet2 Sustains Immunosuppressive Function of Tumor-Infiltrating Myeloid Cells to Promote Melanoma Progression. Immunity. 2017 Aug 15. PMID: 28813659

Zhu S, Ding S, Wang P, Wei Z, Pan W, Palm NW, Yang Y, Yu H, Li HB, Wang G, Lei X, de Zoete MR, Zhao J, Zheng Y, Chen H, Zhao Y, Jurado KA, Feng N, Shan L, Kluger Y, Lu J, Abraham C, Fikrig E, Greenberg HB, Flavell RA: Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells. Nature. 2017 Jun 29; 2017 Jun 21. PMID: 28636595

Cheng J, Pan W, Lu J: Dense sgRNA Library Construction Using a Molecular Chipper Approach. Bio Protoc. 2017 Jun 20. PMID: 28835906

Vuckovic S, Vandyke K, Rickards DA, McCauley Winter P, Brown SHJ, Mitchell TW, Liu J, Lu J, Askenase PW, Yuriev E, Capuano B, Ramsland PA, Hill GR, Zannettino ACW, Hutchinson AT: The cationic small molecule GW4869 is cytotoxic to high phosphatidylserine-expressing myeloma cells. Br J Haematol. 2017 May; 2017 Feb 17. PMID: 28211573

Liu J, Guo B, Chen Z, Wang N, Iacovino M, Cheng J, Roden C, Pan W, Khan S, Chen S, Kyba M, Fan R, Guo S, Lu J: miR-125b promotes MLL-AF9-driven murine acute myeloid leukemia involving a VEGFA-mediated non-cell-intrinsic mechanism. Blood. 2017 Mar 16; 2017 Jan 4. PMID: 28053194

Roden C, Gaillard J, Kanoria S, Rennie W, Barish S, Cheng J, Pan W, Liu J, Cotsapas C, Ding Y, Lu J: Novel determinants of mammalian primary microRNA processing revealed by systematic evaluation of hairpin-containing transcripts and human genetic variation. Genome Res. 2017 Mar; 2017 Jan 13. PMID: 28087842

Liddicoat BJ, Hartner JC, Piskol R, Ramaswami G, Chalk AM, Kingsley PD, Sankaran VG, Wall M, Purton LE, Seeburg PH, Palis J, Orkin SH, Lu J, Li JB, Walkley CR: Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis. Exp Hematol. 2016 Oct; 2016 Jul 1. PMID: 27373493

Roden C, Lu J: MicroRNAs in Control of Stem Cells in Normal and Malignant Hematopoiesis. Curr Stem Cell Rep. 2016 Sep; 2016 Jul 1. PMID: 27547713

Narayanan A, Hill-Teran G, Moro A, Ristori E, Kasper DM, A Roden C, Lu J, Nicoli S: In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system. Sci Rep. 2016 Aug 30; 2016 Aug 30. PMID: 27572667

Hysolli E, Tanaka Y, Su J, Kim KY, Zhong T, Janknecht R, Zhou XL, Geng L, Qiu C, Pan X, Jung YW, Cheng J, Lu J, Zhong M, Weissman SM, Park IH: Regulation of the DNA Methylation Landscape in Human Somatic Cell Reprogramming by the miR-29 Family. Stem Cell Reports. 2016 Jul 12; 2016 Jun 30. PMID: 27373925

Gonzalez-Martin A, Adams BD, Lai M, Shepherd J, Salvador-Bernaldez M, Salvador JM, Lu J, Nemazee D, Xiao C: The microRNA miR-148a functions as a critical regulator of B cell tolerance and autoimmunity. Nat Immunol. 2016 Apr; 2016 Feb 22. PMID: 26901150

Cheng J, Roden CA, Pan W, Zhu S, Baccei A, Pan X, Jiang T, Kluger Y, Weissman SM, Guo S, Flavell RA, Ding Y, Lu J: A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions. Nat Commun. 2016 Mar 30; 2016 Mar 30. PMID: 27025950

Lechman ER, Gentner B, Ng SW, Schoof EM, van Galen P, Kennedy JA, Nucera S, Ciceri F, Kaufmann KB, Takayama N, Dobson SM, Trotman-Grant A, Krivdova G, Elzinga J, Mitchell A, Nilsson B, Hermans KG, Eppert K, Marke R, Isserlin R, Voisin V, Bader GD, Zandstra PW, Golub TR, Ebert BL, Lu J, Minden M, Wang JC, Naldini L, Dick JE: miR-126 Regulates Distinct Self-Renewal Outcomes in Normal and Malignant Hematopoietic Stem Cells. Cancer Cell. 2016 Feb 8; 2016 Jan 28. PMID: 26832662

Kanoria S, Rennie W, Liu C, Carmack CS, Lu J, Ding Y: STarMir Tools for Prediction of microRNA Binding Sites. Methods Mol Biol. 2016. PMID: 27665594

Xiang Y, Cheng J, Wang D, Hu X, Xie Y, Stitham J, Atteya G, Du J, Tang WH, Lee SH, Leslie K, Spollett G, Liu Z, Herzog E, Herzog RI, Lu J, Martin KA, Hwa J: Hyperglycemia repression of miR-24 coordinately upregulates endothelial cell expression and secretion of von Willebrand factor. Blood. 2015 May 28; 2015 Mar 26. PMID: 25814526

Guo Y, Liu J, Elfenbein SJ, Ma Y, Zhong M, Qiu C, Ding Y, Lu J: Characterization of the mammalian miRNA turnover landscape. Nucleic Acids Res. 2015 Feb 27; 2015 Feb 4. PMID: 25653157

Zhang PX, Cheng J, Zou S, D'Souza AD, Koff JL, Lu J, Lee PJ, Krause DS, Egan ME, Bruscia EM: Pharmacological modulation of the AKT/microRNA-199a-5p/CAV1 pathway ameliorates cystic fibrosis lung hyper-inflammation. Nat Commun. 2015 Feb 10; 2015 Feb 10. PMID: 25665524

Roden C, Mastriano S, Wang N, Lu J: microRNA Expression Profiling: Technologies, Insights, and Prospects. Adv Exp Med Biol. 2015. PMID: 26663195

Moskwa P, Zinn PO, Choi YE, Shukla SA, Fendler W, Chen CC, Lu J, Golub TR, Hjelmeland A, Chowdhury D: A functional screen identifies miRs that induce radioresistance in glioblastomas. Mol Cancer Res. 2014 Dec; 2014 Sep 25. PMID: 25256711

Lu J, Cheng L, Lau KC, Tyo E, Luo X, Wen J, Miller D, Assary RS, Wang HH, Redfern P, Wu H, Park JB, Sun YK, Vajda S, Amine K, Curtiss LA: Effect of the size-selective silver clusters on lithium peroxide morphology in lithium-oxygen batteries. Nat Commun. 2014 Sep 12; 2014 Sep 12. PMID: 25215517

Liu C, Rennie WA, Carmack CS, Kanoria S, Cheng J, Lu J, Ding Y: Effects of genetic variations on microRNA: target interactions. Nucleic Acids Res. 2014 Sep; 2014 Jul 31. PMID: 25081214

Rennie W, Liu C, Carmack CS, Wolenc A, Kanoria S, Lu J, Long D, Ding Y: STarMir: a web server for prediction of microRNA binding sites. Nucleic Acids Res. 2014 Jul; 2014 May 6. PMID: 24803672

Guo S, Zi X, Schulz VP, Cheng J, Zhong M, Koochaki SH, Megyola CM, Pan X, Heydari K, Weissman SM, Gallagher PG, Krause DS, Fan R, Lu J: Nonstochastic reprogramming from a privileged somatic cell state. Cell. 2014 Feb 13; 2014 Jan 30. PMID: 24486105

Di Stefano B, Sardina JL, van Oevelen C, Collombet S, Kallin EM, Vicent GP, Lu J, Thieffry D, Beato M, Graf T: C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells. Nature. 2014 Feb 13; 2013 Dec 15. PMID: 24336202

Guo Y, Mastriano S, Lu J: A high-throughput microRNA expression profiling system. Methods Mol Biol. 2014. PMID: 25030917

Cheng J, Guo S, Chen S, Mastriano SJ, Liu C, D'Alessio AC, Hysolli E, Guo Y, Yao H, Megyola CM, Li D, Liu J, Pan W, Roden CA, Zhou XL, Heydari K, Chen J, Park IH, Ding Y, Zhang Y, Lu J: An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis. Cell Rep. 2013 Oct 31; 2013 Oct 10. PMID: 24120864

Megyola CM, Gao Y, Teixeira AM, Cheng J, Heydari K, Cheng EC, Nottoli T, Krause DS, Lu J, Guo S: Dynamic migration and cell-cell interactions of early reprogramming revealed by high-resolution time-lapse imaging. Stem Cells. 2013 May. PMID: 23335078

Stahlhut C, Suárez Y, Lu J, Mishima Y, Giraldez AJ: miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development. 2012 Dec 1. PMID: 23132244

Adams BD, Guo S, Bai H, Guo Y, Megyola CM, Cheng J, Heydari K, Xiao C, Reddy EP, Lu J: An in vivo functional screen uncovers miR-150-mediated regulation of hematopoietic injury response. Cell Rep. 2012 Oct 25; 2012 Oct 19. PMID: 23084747

Guo S, Bai H, Megyola CM, Halene S, Krause DS, Scadden DT, Lu J: Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms. Proc Natl Acad Sci U S A. 2012 Oct 9; 2012 Sep 24. PMID: 23012470