James W Murphy, PhD
Research Scientist in PharmacologyCards
About
Research
Publications
2025
Dynamic and structural insights into allosteric regulation on MKP5 a dual-specificity phosphatase
Skeens E, Maschietto F, Manjula R, Shillingford S, Murphy J, Lolis E, Batista V, Bennett A, Lisi G. Dynamic and structural insights into allosteric regulation on MKP5 a dual-specificity phosphatase. Nature Communications 2025, 16: 7011. PMID: 40745179, PMCID: PMC12313947, DOI: 10.1038/s41467-025-62150-w.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAllosteric SiteCatalytic DomainCrystallography, X-RayDual-Specificity PhosphatasesHumansMagnetic Resonance SpectroscopyMitogen-Activated Protein Kinase PhosphatasesMolecular Dynamics Simulationp38 Mitogen-Activated Protein KinasesPhosphorylationProtein BindingProtein ConformationConceptsMitogen-activated protein kinaseMAPK bindingMolecular mechanismsCatalytic mechanismDual-specificity phosphataseMechanism of dephosphorylationAllosteric siteMolecular dynamics simulationsNMR spectroscopy approachesP38 mitogen-activated protein kinaseCatalytic domainAllosteric regulationRegulatory interplayProtein kinaseCrucial residuesConformational flexibilityDynamics simulationsActive siteStructural insightsMolecular pictureAllosteric pocketDephosphorylationY435MKP5Spectroscopy approach
2021
A structurally preserved allosteric site in the MIF superfamily affects enzymatic activity and CD74 activation in D-dopachrome tautomerase
Chen E, Reiss K, Shah D, Manjula R, Allen B, Murphy EL, Murphy JW, Batista VS, Bhandari V, Lolis EJ, Lisi GP. A structurally preserved allosteric site in the MIF superfamily affects enzymatic activity and CD74 activation in D-dopachrome tautomerase. Journal Of Biological Chemistry 2021, 297: 101061. PMID: 34384784, PMCID: PMC8405996, DOI: 10.1016/j.jbc.2021.101061.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric SiteAmino Acid SequenceAntigens, Differentiation, B-LymphocyteBinding SitesCatalytic DomainCrystallography, X-RayCytokinesHistocompatibility Antigens Class IIHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsProtein BindingStructure-Activity RelationshipConceptsAllosteric siteDopachrome tautomeraseDynamic regulatory networksEnzymatic activityLow sequence identityLigand-binding siteMultiple ligand-binding sitesNonoverlapping functionsRegulatory networksAllosteric couplingMacrophage migration inhibitory factor (MIF) familyFactor familySequence identityHomolog DStructural basisPrimary sequenceCD74 activationFunctional similarityConformational changesSolution NMRMIF-2X-ray crystallographyCatalytic siteStructural consequencesSolvent channels
2007
The Structural Biology of Chemokines
Lolis E, Murphy J. The Structural Biology of Chemokines. The Receptors 2007, 9-30. DOI: 10.1007/978-1-59745-020-1_2.Peer-Reviewed Original Research