2024
Lung Transcriptomics Links Emphysema to Barrier Dysfunction and Macrophage Subpopulations.
Lu R, Gregory A, Suryadevara R, Xu Z, Jain D, Morrow J, Hobbs B, Yun J, Lichtblau N, Chase R, Curtis J, Sauler M, Bartholmai B, Silverman E, Hersh C, Castaldi P, Boueiz A. Lung Transcriptomics Links Emphysema to Barrier Dysfunction and Macrophage Subpopulations. American Journal Of Respiratory And Critical Care Medicine 2024 PMID: 38935868, DOI: 10.1164/rccm.202305-0793oc.Peer-Reviewed Original ResearchRNA sequencing dataAlternative splicingExpressed genesFunctional pathwaysCell typesBiological pathwaysGene expressionTranscriptomic featuresGene regulatory processesDysregulated pathwaysSingle-cell RNA sequencing dataRNA-seq analysisLung cell typesLung Tissue Research ConsortiumTranscriptome analysisGenesCell-typeDifferential expressionMultiple lung cell typesPathway activationTranscriptomic signaturesPathway dysregulationRegulatory processesSplicingPathway
2021
Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung
Schupp JC, Adams TS, Cosme C, Raredon MSB, Yuan Y, Omote N, Poli S, Chioccioli M, Rose KA, Manning EP, Sauler M, DeIuliis G, Ahangari F, Neumark N, Habermann AC, Gutierrez AJ, Bui LT, Lafyatis R, Pierce RW, Meyer KB, Nawijn MC, Teichmann SA, Banovich NE, Kropski JA, Niklason LE, Pe’er D, Yan X, Homer RJ, Rosas IO, Kaminski N. Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung. Circulation 2021, 144: 286-302. PMID: 34030460, PMCID: PMC8300155, DOI: 10.1161/circulationaha.120.052318.Peer-Reviewed Original ResearchConceptsDifferential expression analysisPrimary lung endothelial cellsLung endothelial cellsCell typesMarker genesExpression analysisSingle-cell RNA sequencing dataCross-species analysisVenous endothelial cellsEndothelial marker genesSingle-cell atlasMarker gene setsRNA sequencing dataEndothelial cellsSubsequent differential expression analysisDifferent lung cell typesResident cell typesLung cell typesCellular diversityEndothelial cell typesCapillary endothelial cellsHuman lung endothelial cellsPhenotypic diversityEndothelial diversityIndistinguishable populations