Featured Publications
Endothelial CD74 mediates macrophage migration inhibitory factor protection in hyperoxic lung injury
Sauler M, Zhang Y, Min J, Leng L, Shan P, Roberts S, Jorgensen WL, Bucala R, Lee PJ. Endothelial CD74 mediates macrophage migration inhibitory factor protection in hyperoxic lung injury. The FASEB Journal 2015, 29: 1940-1949. PMID: 25609432, PMCID: PMC4415022, DOI: 10.1096/fj.14-260299.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorLung endothelial cellsMurine lung endothelial cellsLung injuryEndothelial cellsProtective effectExogenous MIFCD74 deficient miceCD74-dependent mannerHours of hyperoxiaMIF receptor CD74Acute lung injuryHyperoxic lung injuryBronchoalveolar lavage proteinMigration inhibitory factorBronchoalveolar proteinMIF deficiencyMedian survivalEndothelial injuryWT miceAcute oxidative stressReceptor CD74Lavage proteinReceptor antagonistReceptor inhibitors
2022
Sex differences and altered mitophagy in experimental pulmonary hypertension
Bazan IS, Kim SJ, Ardito TA, Zhang Y, Shan P, Sauler M, Lee P. Sex differences and altered mitophagy in experimental pulmonary hypertension. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2022, 322: l761-l769. PMID: 35137625, PMCID: PMC9076415, DOI: 10.1152/ajplung.00019.2020.Peer-Reviewed Original ResearchConceptsPulmonary hypertensionPathogenesis of PHFemale-predominant diseaseRight heart failurePulmonary arterial pressureExperimental pulmonary hypertensionMitochondrial dysfunctionLung endothelial cellsSex differencesMouse lung endothelial cellsInhibition of ParkinArterial pressureHeart failurePulmonary arteryMitochondrial respiratory capacityPoor outcomeHigh prevalenceSevere diseaseAnimal modelsEndothelial cellsParkin expressionOxidative stressCell proliferationHypertensionSexCharacterization of pulmonary vascular remodeling and MicroRNA-126-targets in COPD-pulmonary hypertension
Goel K, Egersdorf N, Gill A, Cao D, Collum S, Jyothula S, Huang H, Sauler M, Lee P, Majka S, Karmouty-Quintana H, Petrache I. Characterization of pulmonary vascular remodeling and MicroRNA-126-targets in COPD-pulmonary hypertension. Respiratory Research 2022, 23: 349. PMID: 36522710, PMCID: PMC9756782, DOI: 10.1186/s12931-022-02267-4.Peer-Reviewed Original ResearchConceptsSmall pulmonary arteriesChronic cigarette smokingPulmonary vascular remodelingPulmonary hypertensionPulmonary arteryLung microvasculatureVascular remodelingEndothelial cellsCOPD-PHMiR126 expressionChronic obstructive pulmonary disease patientsObstructive pulmonary disease patientsCOPD pulmonary hypertensionPulmonary artery sizePulmonary artery remodelingPulmonary disease patientsPulmonary vascular bedPulmonary arterial wallEndothelial cell marker CD31Microvascular endothelial cellsEndothelial cell markersVessel endothelial cellsNon-COPD lungsLarge vessel endothelial cellsArtery remodeling
2021
Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung
Schupp JC, Adams TS, Cosme C, Raredon MSB, Yuan Y, Omote N, Poli S, Chioccioli M, Rose KA, Manning EP, Sauler M, DeIuliis G, Ahangari F, Neumark N, Habermann AC, Gutierrez AJ, Bui LT, Lafyatis R, Pierce RW, Meyer KB, Nawijn MC, Teichmann SA, Banovich NE, Kropski JA, Niklason LE, Pe’er D, Yan X, Homer RJ, Rosas IO, Kaminski N. Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung. Circulation 2021, 144: 286-302. PMID: 34030460, PMCID: PMC8300155, DOI: 10.1161/circulationaha.120.052318.Peer-Reviewed Original ResearchConceptsDifferential expression analysisPrimary lung endothelial cellsLung endothelial cellsCell typesMarker genesExpression analysisSingle-cell RNA sequencing dataCross-species analysisVenous endothelial cellsEndothelial marker genesSingle-cell atlasMarker gene setsRNA sequencing dataEndothelial cellsSubsequent differential expression analysisDifferent lung cell typesResident cell typesLung cell typesCellular diversityEndothelial cell typesCapillary endothelial cellsHuman lung endothelial cellsPhenotypic diversityEndothelial diversityIndistinguishable populations
2018
Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation
Pellowe AS, Sauler M, Hou Y, Merola J, Liu R, Calderon B, Lauridsen HM, Harris MR, Leng L, Zhang Y, Tilstam PV, Pober JS, Bucala R, Lee PJ, Gonzalez AL. Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation. The FASEB Journal 2018, 33: 2171-2186. PMID: 30252532, PMCID: PMC6338650, DOI: 10.1096/fj.201800480r.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorNeutrophil transmigrationNeutrophil extravasationPericyte contractilitySelective deletionMIF knockout miceAcute lung injuryMigration inhibitory factorHuman neutrophil transmigrationMyosin light chain phosphorylationLung injuryNeutrophil infiltrationBronchoalveolar lavageInflammatory disordersKnockout miceInhibitory factorMicro-ELISAContractilityLight chain phosphorylationPhosphorylated myosin light chainEndothelial cellsBarrier functionExtravasationPericytesParacrine signals
2013
Lung endothelial HO‐1 targeting in vivo using lentiviral miRNA regulates apoptosis and autophagy during oxidant injury
Zhang Y, Jiang G, Sauler M, Lee PJ. Lung endothelial HO‐1 targeting in vivo using lentiviral miRNA regulates apoptosis and autophagy during oxidant injury. The FASEB Journal 2013, 27: 4041-4058. PMID: 23771928, PMCID: PMC4046184, DOI: 10.1096/fj.13-231225.Peer-Reviewed Original ResearchConceptsEndothelial HO-1Murine lung endothelial cellsHO-1Lung endotheliumHeme oxygenase-1 inductionHO-1 knockdownLung endothelial cellsOxygenase-1 inductionLung cell typesLung injuryWT miceMultiple lung cell typesVascular endothelium cadherinOxidant injuryCrucial defense mechanismHyperoxiaEndothelial cellsOxidative stressOxidant challengeEndotheliumMajor targetROS generationInjuryLentiviral vectorsVivo