2001
Conserved CDR3 Regions in T-Cell Receptor (TCR) CD8+T Cells That Recognize the Tax11-19/HLA-A*0201 Complex in a Subject Infected with Human T-Cell Leukemia Virus Type 1: Relationship of T-Cell Fine Specificity and Major Histocompatibility Complex/Peptide/TCR Crystal Structure
Bourcier K, Lim D, Ding Y, Smith K, Wucherpfennig K, Hafler D. Conserved CDR3 Regions in T-Cell Receptor (TCR) CD8+T Cells That Recognize the Tax11-19/HLA-A*0201 Complex in a Subject Infected with Human T-Cell Leukemia Virus Type 1: Relationship of T-Cell Fine Specificity and Major Histocompatibility Complex/Peptide/TCR Crystal Structure. Journal Of Virology 2001, 75: 9836-9843. PMID: 11559817, PMCID: PMC114556, DOI: 10.1128/jvi.75.20.9836-9843.2001.Peer-Reviewed Original ResearchConceptsT cell clonesT cell receptorCDR3 regionTCR alphaHTLV-1-associated myelopathyTax11-19Beta chainHTLV-1-infected individualsT cell receptor repertoirePeripheral blood lymphocytesHuman T-cell leukemia virusHuman T-cell leukemia virus type 1T-cell leukemia virusVirus type 1TCR usageT cell fine specificityBlood lymphocytesT cellsImmunodominant epitopesReceptor repertoireType 1Fine specificitySimilar recognition patternTCR structureLeukemia virus
2000
Examination of CD8+ T Cell Function in Humans Using MHC Class I Tetramers: Similar Cytotoxicity but Variable Proliferation and Cytokine Production Among Different Clonal CD8+ T Cells Specific to a Single Viral Epitope
Lim D, Bourcier K, Freeman G, Hafler D. Examination of CD8+ T Cell Function in Humans Using MHC Class I Tetramers: Similar Cytotoxicity but Variable Proliferation and Cytokine Production Among Different Clonal CD8+ T Cells Specific to a Single Viral Epitope. The Journal Of Immunology 2000, 165: 6214-6220. PMID: 11086055, DOI: 10.4049/jimmunol.165.11.6214.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, ViralB7-1 AntigenCD58 AntigensCD8-Positive T-LymphocytesCell Line, TransformedClone CellsCytokinesCytotoxicity, ImmunologicDose-Response Relationship, ImmunologicEpitopes, T-LymphocyteGene Products, taxGenes, T-Cell Receptor betaHLA-A2 AntigenHuman T-lymphotropic virus 1HumansLymphocyte ActivationPeptide FragmentsStaining and LabelingConceptsT cell clonesCytokine secretionT cellsEffector functionsCell clonesCostimulatory moleculesViral epitopesHuman T-cell lymphotrophic virusDifferent T cell clonesImmunodominant viral epitopesCytotoxic effector functionClonal originT cell functionSingle viral epitopeMHC class IDifferent clonal originCD2-LFA-3 interactionInduction of proliferationClonal CD8Cytokine productionPeripheral bloodCTL populationsIL-2Lymphotrophic virusProliferative response
1999
The distinction blurs between an autoimmune versus microbial hypothesis in multiple sclerosis
Hafler DA. The distinction blurs between an autoimmune versus microbial hypothesis in multiple sclerosis. Journal Of Clinical Investigation 1999, 104: 527-529. PMID: 10487765, PMCID: PMC483283, DOI: 10.1172/jci8193.Peer-Reviewed Original ResearchAnimalsAntigen PresentationAntigens, ViralAutoantigensAutoimmune DiseasesCardiovirus InfectionsCross ReactionsDemyelinating DiseasesDisease Models, AnimalDisease ProgressionEncephalomyelitis, Autoimmune, ExperimentalEpitopesHuman T-lymphotropic virus 1HumansMiceMicrogliaModels, ImmunologicalMolecular MimicryMultiple SclerosisMyelin ProteinsParaparesis, Tropical SpasticT-Lymphocyte SubsetsTheilovirusVirus DiseasesHTLV-I-Infected T Cells Evade the Antiproliferative Action of IFN-β
Smith D, Buckle G, Hafler D, Frank D, Höllsberg P. HTLV-I-Infected T Cells Evade the Antiproliferative Action of IFN-β. Virology 1999, 257: 314-321. PMID: 10329542, DOI: 10.1006/viro.1999.9679.Peer-Reviewed Original ResearchConceptsT cell clonesIFN-betaT cellsAntiproliferative actionHuman T-cell lymphotropic virus type IMyelopathy/tropical spastic paraparesisLymphotropic virus type INormal immunoregulatory mechanismsInnate immune defense mechanismsHost T cellsExogenous IL-2Tropical spastic paraparesisT cell proliferationImmune defense mechanismsVirus type IT cell activationPathogenesis of HTLVImmunoregulatory mechanismsSpastic paraparesisIL-2Phosphorylation of STAT1IFN-gammaViral infectionHigh dosesHTLVDirect analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy.
Bieganowska K, Höllsberg P, Buckle G, Lim D, Greten T, Schneck J, Altman J, Jacobson S, Ledis S, Hanchard B, Chin J, Morgan O, Roth P, Hafler D. Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy. The Journal Of Immunology 1999, 162: 1765-71. PMID: 9973440, DOI: 10.4049/jimmunol.162.3.1765.Peer-Reviewed Original ResearchMeSH KeywordsAdultB7-1 AntigenCD28 AntigensCD8-Positive T-LymphocytesFemaleGene Products, taxHLA-A2 AntigenHuman T-lymphotropic virus 1HumansLymphocyte ActivationMaleMiddle AgedParaparesis, Tropical SpasticPeptide FragmentsPhenotypeProtein ConformationReceptors, Antigen, T-Cell, alpha-betaReceptors, ChemokineReceptors, Interleukin-2SolubilityConceptsT cellsIL-2RMHC class I tetramersHuman T-cell lymphotropic virusViral-specific CD8Class I tetramersProgressive neurologic diseaseDifferent chemokine receptorsHLA-A2 alleleCentral nervous systemTCR Vbeta chainsReactive CD8TCR usageInflammatory infiltrateVbeta chainsChemokine receptorsNeurologic diseaseImmune responseCD8Lymphotropic virusMyelopathyNervous systemPatientsHTLVClonal expansion
1997
Variable Immortalizing Potential and Frequent Virus Latency in Blood-Derived T-Cell Clones Infected With Human T-Cell Leukemia Virus Type I
Richardson JH, Höllsberg P, Windhagen A, Child LA, Hafler DA, Lever A. Variable Immortalizing Potential and Frequent Virus Latency in Blood-Derived T-Cell Clones Infected With Human T-Cell Leukemia Virus Type I. Blood 1997, 89: 3303-3314. PMID: 9129036, DOI: 10.1182/blood.v89.9.3303.Peer-Reviewed Original ResearchConceptsT cell clonesHuman T-cell leukemia virus type IVirus type IT cellsSpontaneous proliferationFresh peripheral blood lymphocytesGag p24 productionCD4 T cellsInfected cellsPeripheral blood lymphocytesHTLV-I provirusViral mRNA expressionType IP24 productionVirus-cell interactionsIL-6Virus carriersInterleukin-6Blood lymphocytesHost cell factorsCytokine mRNAVirus latencyVirus-producing clonesHTLVMRNA expression
1996
Activation of human T cell lymphotropic virus type I-infected T cells is independent of B7 costimulation.
Scholz C, Freeman GJ, Greenfield EA, Hafler DA, Höllsberg P. Activation of human T cell lymphotropic virus type I-infected T cells is independent of B7 costimulation. The Journal Of Immunology 1996, 157: 2932-8. PMID: 8816399, DOI: 10.4049/jimmunol.157.7.2932.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigen-Presenting CellsAntigens, CDAutoimmunityB7-1 AntigenB7-2 AntigenBase SequenceCD28 AntigensCHO CellsClone CellsCricetinaeCricetulusEnzyme ActivationHLA-DR AntigensHLA-DRB1 ChainsHuman T-lymphotropic virus 1HumansInterferon-gammaInterleukin-4Interleukin-5Janus Kinase 3Lymphocyte ActivationMembrane GlycoproteinsMolecular Sequence DataMyelin Basic ProteinProtein-Tyrosine KinasesSignal TransductionT-Lymphocyte SubsetsTransfectionConceptsHuman T-cell lymphotropic virus type ILymphotropic virus type IB7 costimulationT cell clonesT cellsB7-1Virus type IIL-4IL-5B7-2IFN-gammaAutoreactive T cell responsesCell clonesAg-specific signalAutoimmune-like diseaseT cell responsesAutoreactive T cellsHTLV-I infectionB7-2 costimulationB7-2 moleculesUninfected T cellsType IAutoimmune responseB7 expressionCytokine secretionHTLV-I-induced T-cell activation.
Buckle GJ, Hafler DA, Höllsberg P. HTLV-I-induced T-cell activation. JAIDS Journal Of Acquired Immune Deficiency Syndromes 1996, 13 Suppl 1: s107-13. PMID: 8797712, DOI: 10.1097/00042560-199600001-00018.Peer-Reviewed Original ResearchConceptsT cell activationT cellsUninfected T cellsT cell interactionsMyelopathy/tropical spastic paraparesisHuman T-cell lymphotropic virus type ILymphotropic virus type ITropical spastic paraparesisInfected T cellsT cell clonesIL-2 productionInterleukin-2 productionVirus type ISpastic paraparesisSpontaneous proliferationCD28 pathwayGeneralized activationCostimulatory signalsImmune systemSeparate mechanismsHTLVProlonged stateType IActivationCells
1995
Differential activation of proliferation and cytotoxicity in human T-cell lymphotropic virus type I Tax-specific CD8 T cells by an altered peptide ligand.
Höllsberg P, Weber WE, Dangond F, Batra V, Sette A, Hafler DA. Differential activation of proliferation and cytotoxicity in human T-cell lymphotropic virus type I Tax-specific CD8 T cells by an altered peptide ligand. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4036-4040. PMID: 7732026, PMCID: PMC42097, DOI: 10.1073/pnas.92.9.4036.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceB-LymphocytesBinding SitesCD8-Positive T-LymphocytesCell DivisionCell Line, TransformedCytotoxicity, ImmunologicGene Products, taxHerpesvirus 4, HumanHLA-A2 AntigenHuman T-lymphotropic virus 1HumansKineticsLymphocyte ActivationMolecular Sequence DataMutagenesis, Site-DirectedPoint MutationThymidineConceptsCD8 T cellsInterleukin-2 secretionT cell receptor contact residuesT cellsReceptor contact residuesCD8 T cell clonesCytotoxic CD8 T cellsMyelopathy/tropical spastic paraparesisT cell-mediated cytotoxicityHAM/TSPTropical spastic paraparesisT cell clonesAltered peptide ligandT cell-mediated cytolysisHuman T-cell leukemia virus type IT cell stimulationVirus type IAnalog peptidesHLA-A2Spastic paraparesisNeurologic diseaseSecondary stimulationSecondary responsePeptide antigensPrimary activationWhat is the pathogenesis of human T‐cell lymphotropic virus type I–associated myelopathy/tropical spastic paraparesis?
Höllsberg P, Hafler D. What is the pathogenesis of human T‐cell lymphotropic virus type I–associated myelopathy/tropical spastic paraparesis? Annals Of Neurology 1995, 37: 143-145. PMID: 7847855, DOI: 10.1002/ana.410370203.Peer-Reviewed Original ResearchMeSH KeywordsAutoimmunityCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesHuman T-lymphotropic virus 1HumansParaparesis, Tropical Spastic
1994
Human T cell lymphotropic virus type I-induced T cell activation. Resistance to TGF-beta 1-induced suppression.
Höllsberg P, Ausubel LJ, Hafler DA. Human T cell lymphotropic virus type I-induced T cell activation. Resistance to TGF-beta 1-induced suppression. The Journal Of Immunology 1994, 153: 566-73. PMID: 8021495, DOI: 10.4049/jimmunol.153.2.566.Peer-Reviewed Original ResearchConceptsT cell clonesT cell activationHuman T-cell lymphotropic virus type ILymphotropic virus type IVirus type ICell activationCell clonesT cellsCD3/TCR complexHTLV-I myelopathyT cell proliferationType IImmune regulationHTLVHyperphosphorylation of pRbProductive infectionCell cycle progressionCell proliferationTCR complexPatientsG1 phaseInfectionSingle cell cloningCycle progressionActivationRegional specificity of HTLV-I pro viral integration in the human genome
Zoubak S, Richardson JH, Rynditch A, Hollsberg P, Hafler DA, Boeri E, Lever A, Bernardi G. Regional specificity of HTLV-I pro viral integration in the human genome. Gene 1994, 143: 155-163. PMID: 8206368, DOI: 10.1016/0378-1119(94)90091-4.Peer-Reviewed Original Research
1993
Pathogenesis of Diseases Induced by Human Lymphotropic Virus Type I Infection
Flier J, Underhill L, Hollsberg P, Hafler D. Pathogenesis of Diseases Induced by Human Lymphotropic Virus Type I Infection. New England Journal Of Medicine 1993, 328: 1173-1182. PMID: 8455685, DOI: 10.1056/nejm199304223281608.Peer-Reviewed Original Research
1992
T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones.
Wucherpfennig KW, Höllsberg P, Richardson JH, Benjamin D, Hafler DA. T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 2110-2114. PMID: 1549569, PMCID: PMC48606, DOI: 10.1073/pnas.89.6.2110.Peer-Reviewed Original ResearchConceptsIntercellular cell adhesion moleculeBlood T cellsT cell clonesT cell activationIL-2 receptorT cellsActivated T cellsHuman T-cell leukemia virus type I (HTLV-I) carriersSuch activated T cellsHAM/TSP patientsMyelopathy/tropical spastic paraparesisClass II major histocompatibility complex moleculesHAM/TSPChronic inflammatory diseaseExogenous interleukin-2HTLV-I infectionTropical spastic paraparesisMajor histocompatibility complex moleculesHuman T-cell leukemia virus type IVirus type IHistocompatibility complex moleculesCD2/LFATSP patientsSpastic paraparesisSpontaneous proliferation