2022
Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Marsh SE, Walker AJ, Kamath T, Dissing-Olesen L, Hammond TR, de Soysa TY, Young AMH, Murphy S, Abdulraouf A, Nadaf N, Dufort C, Walker AC, Lucca LE, Kozareva V, Vanderburg C, Hong S, Bulstrode H, Hutchinson PJ, Gaffney DJ, Hafler DA, Franklin RJM, Macosko EZ, Stevens B. Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain. Nature Neuroscience 2022, 25: 306-316. PMID: 35260865, DOI: 10.1038/s41593-022-01022-8.Peer-Reviewed Original ResearchConceptsSingle-cell sequencing experimentsCell type diversitySingle-cell sequencingRNA-sequencing datasetsGene expression changesGene expression signaturesVivo gene expressionTranscriptional profilesGene expressionExpression changesSequencing experimentsGlial signaturesDownstream analysisExpression signaturesTissue typesSingle cell suspensionsOptimal cell yieldIntact tissueHuman tissuesCell yieldEnzymatic dissociationHuman samplesTissue digestionPostmortem human samplesTissue
2009
On-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cells
Song Q, Han Q, Bradshaw EM, Kent SC, Raddassi K, Nilsson B, Nepom GT, Hafler DA, Love JC. On-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cells. Analytical Chemistry 2009, 82: 473-477. PMID: 20000848, PMCID: PMC2828941, DOI: 10.1021/ac9024363.Peer-Reviewed Original ResearchConceptsAntigen-specific T cellsAntigen-specific CD4T cellsAntigen-specific mannerType 1 diabetesMajor histocompatibility complex receptorIndividual CD4Multiple sclerosisAutoimmune diseasesPrimary T cellsCD4Subnanoliter wellsClinical samplesNumber of cellsComplex receptorSubsequent assessmentHuman tissuesCellsChip activationVivoActivationSclerosisDiabetesCytokinesDisease