2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2022
Gut colonization by Bacteroides requires translation by an EF‐G paralog lacking GTPase activity
Han W, Peng B, Wang C, Townsend G, Barry N, Peske F, Goodman A, Liu J, Rodnina M, Groisman E. Gut colonization by Bacteroides requires translation by an EF‐G paralog lacking GTPase activity. The EMBO Journal 2022, 42: embj2022112372. PMID: 36472247, PMCID: PMC9841332, DOI: 10.15252/embj.2022112372.Peer-Reviewed Original ResearchConceptsEF-G1Protein synthesisGTPase activityGuanosine triphosphateElongation factor GCarbon starvationCellular processesStarvation conditionsBacteroides thetaiotaomicronFactor GSingular abilityAmino acidsCell growthParalogsMurine cecumTranslocationGut colonizationColonizationCellsRibosomesProteinStarvationThetaiotaomicronBacteriaFitness
2021
The regulation of DNA supercoiling across evolution
Duprey A, Groisman EA. The regulation of DNA supercoiling across evolution. Protein Science 2021, 30: 2042-2056. PMID: 34398513, PMCID: PMC8442966, DOI: 10.1002/pro.4171.Peer-Reviewed Original ResearchConceptsDomains of lifeDNA supercoilingCellular processesAbnormal DNA replicationCertain cellular processesActivity of topoisomerasesChromosome replicationGlobal supercoilingNegative supercoilsDNA replicationBiological questionsSupercoilingEukaryotic topoisomerasesTopoisomerasesSupercoilsSignificant therapeutic potentialOrganismsHelix structureBacteriaPhysicochemical factorsRegulationDouble helix structureReplicationUnique strategyArchaeaLimitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis
Bruna RE, Kendra CG, Groisman EA, Pontes MH. Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2021370118. PMID: 33707210, PMCID: PMC7980370, DOI: 10.1073/pnas.2021370118.Peer-Reviewed Original ResearchConceptsCytoplasmic MgPhosphate assimilationRibosomal RNARegulatory logicP assimilationMolecular basisLoss of viabilityProtein inhibitsPi toxicityAdenosine triphosphateATP synthesisProtein synthesisATP accumulationHomeostasisBacterial growthCytosolic PiDependent processesMagnesium homeostasisBacteriaBiological moleculesInorganic orthophosphateEssential componentAssimilationGrowthRNA
2019
Slow growth determines nonheritable antibiotic resistance in Salmonella enterica
Pontes MH, Groisman EA. Slow growth determines nonheritable antibiotic resistance in Salmonella enterica. Science Signaling 2019, 12 PMID: 31363068, PMCID: PMC7206539, DOI: 10.1126/scisignal.aax3938.Peer-Reviewed Original ResearchConceptsToxin-antitoxin modulesSlow growthGenetic repertoireSuch proteinsPersister formationAntibiotic-resistant mutantsParticular proteinPersister statePhysiological processesPersistent bacteriaPhenotypic changesBacterial populationsCell growthUse of antibioticsSalmonella entericaBactericidal antibioticsBacteriaProteinSmall subpopulationAbundanceUnderlying physiological processesAntibiotic resistanceGrowthMutantsOpposite changes
2017
Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate
Yeom J, Wayne KJ, Groisman EA. Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate. Molecular Cell 2017, 66: 234-246.e5. PMID: 28431231, PMCID: PMC5424706, DOI: 10.1016/j.molcel.2017.03.009.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBinding, CompetitiveCation Transport ProteinsEndopeptidase ClpGene Expression Regulation, BacterialHalf-LifeModels, MolecularMutationProtein BindingProtein Interaction Domains and MotifsProtein StabilityProteolysisSalmonella typhimuriumStructure-Activity RelationshipSubstrate SpecificityTime FactorsTranscription, GeneticConceptsN-terminal residuesSpecific N-terminal residuesRegulatory protein PhoPN-end ruleProtease ClpAPTemporal transcriptionMgtC geneN-terminusPhoPSpecific substratesClpAPDifferential stabilityProtein levelsGenesMgtCResiduesTranscriptionTerminusProteolysisProteinSubstrateProteaseBacteriaDegradationSequestration
2013
The Biology of the PmrA/PmrB Two-Component System: The Major Regulator of Lipopolysaccharide Modifications
Chen HD, Groisman EA. The Biology of the PmrA/PmrB Two-Component System: The Major Regulator of Lipopolysaccharide Modifications. Annual Review Of Microbiology 2013, 67: 83-112. PMID: 23799815, PMCID: PMC8381567, DOI: 10.1146/annurev-micro-092412-155751.Peer-Reviewed Original ResearchConceptsPmrA/PmrBTwo-component systemPmrA/PmrB systemMajor regulatorEnteric pathogen Salmonella entericaGene expression outputPathogen Salmonella entericaTranscriptional regulatorsExpression outputEcological nichesOuter membraneRelated bacteriaLPS modificationsExtent bacteriaLipopolysaccharide modificationDifferential survivalRegulatorHost immune systemNegative bacteriaSalmonella entericaBacteriaBiologyPmrBQuantitative differencesImmune system
2012
Reciprocal Control between a Bacterium's Regulatory System and the Modification Status of Its Lipopolysaccharide
Kato A, Chen HD, Latifi T, Groisman EA. Reciprocal Control between a Bacterium's Regulatory System and the Modification Status of Its Lipopolysaccharide. Molecular Cell 2012, 47: 897-908. PMID: 22921935, PMCID: PMC3465083, DOI: 10.1016/j.molcel.2012.07.017.Peer-Reviewed Original Research
2009
Evolution of Transcriptional Regulatory Circuits in Bacteria
Perez JC, Groisman EA. Evolution of Transcriptional Regulatory Circuits in Bacteria. Cell 2009, 138: 233-244. PMID: 19632175, PMCID: PMC2726713, DOI: 10.1016/j.cell.2009.07.002.Peer-Reviewed Original ResearchConceptsTranscription factorsRegulatory circuitsOrthologous transcription factorsBacterial regulatory circuitsAncestral transcription factorTranscriptional regulatory circuitsDistinct gene setsHorizontal gene transferSpecies-specific genesEukaryotic speciesPromoter structureRelated organismsGene setsRegulatory differencesGene transferGenesCircuit evolutionBacteriaOrganismsRewiringSpeciesDivergenceEvolutionExpressionCues
2008
Evolution and Dynamics of Regulatory Architectures Controlling Polymyxin B Resistance in Enteric Bacteria
Mitrophanov AY, Jewett MW, Hadley TJ, Groisman EA. Evolution and Dynamics of Regulatory Architectures Controlling Polymyxin B Resistance in Enteric Bacteria. PLOS Genetics 2008, 4: e1000233. PMID: 18949034, PMCID: PMC2565834, DOI: 10.1371/journal.pgen.1000233.Peer-Reviewed Original ResearchConceptsRegulatory architectureComplex genetic networksDirect transcriptional controlEnteric bacteriaPolymyxin B resistanceEvolutionary historyTranscriptional controlGenetic networksRegulatory proteinsRegulatory pathwaysCellular responsesStructural modulesIndirect regulationRapid activationB resistanceExpression levelsMRNA expression levelsAntibiotic polymyxin BRegulation circuitBacteriaInducible resistanceFunctional propertiesProteinSystem postTransitional stageSignal integration in bacterial two-component regulatory systems
Mitrophanov AY, Groisman EA. Signal integration in bacterial two-component regulatory systems. Genes & Development 2008, 22: 2601-2611. PMID: 18832064, PMCID: PMC2751022, DOI: 10.1101/gad.1700308.Peer-Reviewed Original ResearchConceptsTwo-component systemResponse regulatorTwo-component regulatory systemSignal integrationBacterial signal transductionGram-negative bacteriaCellular processesSignal transductionPhosphorylated statePhosphorylation statePhysiological functionsSpecific functionsRegulatory systemBiochemical reactionsKey mediatorRegulatorPhosphorelayAntibiotic resistanceDifferent mechanismsSporulationTransductionStationary phaseDNABacteriaGram
2003
Making informed decisions: regulatory interactions between two-component systems
Bijlsma JJ, Groisman EA. Making informed decisions: regulatory interactions between two-component systems. Trends In Microbiology 2003, 11: 359-366. PMID: 12915093, DOI: 10.1016/s0966-842x(03)00176-8.Peer-Reviewed Original ResearchConceptsTwo-component systemTwo-component regulatory systemPost-transcriptional mechanismsMultitude of stressesResponse regulatorBacterial adaptationRegulatory interactionsComplex nichePhosphoryl transferMolecular levelRegulatory systemSpecific signalsMultiple signalsPhosphorelayMultifaceted environmentNicheRegulatorBacteriaPhosphataseMechanismStressAdaptation
2000
Lateral gene transfer and the nature of bacterial innovation
Ochman H, Lawrence J, Groisman E. Lateral gene transfer and the nature of bacterial innovation. Nature 2000, 405: 299-304. PMID: 10830951, DOI: 10.1038/35012500.Peer-Reviewed Original ResearchConceptsLateral gene transferGene transferHorizontal gene transferBacterial innovationDynamic genomeGenetic diversityAcquisition of sequencesGenetic informationLateral transferBacterial speciesPathogenic characterEukaryotesGenomeChromosomesOrganismsSpeciesDNADiversityBacteriaSequenceSubstantial amount
1995
The evolution off invasion by enteric bacteria
Ochman H, Groisman EA. The evolution off invasion by enteric bacteria. Canadian Journal Of Microbiology 1995, 41: 555-561. PMID: 7641138, DOI: 10.1139/m95-074.Peer-Reviewed Original ResearchConceptsPhylogenetic distributionGenomic locationPlant pathogensDivergent pathogensSecretory pathwayVirulence proteinsGene complexHost rangeHost cellsSimilar machineryEnteric bacteriaPathogensProteinDisease pathologyEnteric pathogensMachineryBacteriaPathwayShigella sppSppAnalogous structuresSequenceInvasionCellsComplexes
1994
How bacteria resist killing by host-defense peptides
Groisman E. How bacteria resist killing by host-defense peptides. Trends In Microbiology 1994, 2: 444-449. PMID: 7866702, DOI: 10.1016/0966-842x(94)90802-8.Peer-Reviewed Original Research