1999
Phosphatidylinositol 3-Phosphate Recognition by the FYVE Domain
Kutateladze T, Ogburn K, Watson W, de Beer T, Emr S, Burd C, Overduin M. Phosphatidylinositol 3-Phosphate Recognition by the FYVE Domain. Molecular Cell 1999, 3: 805-811. PMID: 10394369, DOI: 10.1016/s1097-2765(01)80013-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesConserved SequenceDimerizationHumansLiposomesMembrane ProteinsMolecular Sequence DataMolecular WeightMutationNuclear Magnetic Resonance, BiomolecularPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphatidylinositolsProtein BindingProtein FoldingProtein Structure, SecondarySolubilitySubstrate SpecificityVesicular Transport ProteinsZincZinc FingersConceptsFYVE domainEndosome autoantigen 1Membrane trafficking eventsHeteronuclear magnetic resonance spectroscopyZinc-binding motifSpecific amino acidsTrafficking eventsEndosome fusionCellular signalingAlpha-helixBeta hairpinAmino acidsPhosphoinositidePhosphatidylinositolMotifStructural featuresRing fingerDomainPtdlnsSignalingProteinHairpinHelixBroad rangeLipidsVac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
Peterson M, Burd C, Emr S. Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Current Biology 1999, 9: 159-s1. PMID: 10021387, DOI: 10.1016/s0960-9822(99)80071-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCytoskeletal ProteinsEndosomesFungal ProteinsGolgi ApparatusGTP PhosphohydrolasesGTP-Binding ProteinsGuanine Nucleotide Dissociation InhibitorsGuanosine TriphosphateMacromolecular SubstancesMembrane ProteinsPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesProtein BindingQa-SNARE ProteinsRab GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsVacuolar protein sorting (VPS) pathwayVesicle docking/fusionProtein sorting pathwaysDocking/fusionVacuolar protein precursorsMultivalent adaptor proteinLysosome-like vacuolePrevacuolar endosomeVPS genesVps34 phosphatidylinositolLate GolgiGTPase signalsRab GTPaseSorting pathwaysVesicle dockingEndosome transportVacuolar proteinAdaptor proteinEndosomal compartmentsVac1pProtein precursorGolgiTransport stepsVps21pVps45p
1998
Acidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE
Darsow T, Burd C, Emr S. Acidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE. Journal Of Cell Biology 1998, 142: 913-922. PMID: 9722605, PMCID: PMC2132875, DOI: 10.1083/jcb.142.4.913.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAlkaline PhosphataseBiological TransportCarboxypeptidasesCathepsin AEndosomesFungal ProteinsLeucineMembrane ProteinsMonomeric Clathrin Assembly ProteinsMutationNerve Tissue ProteinsPhosphoproteinsQa-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSNARE ProteinsVesicle-Associated Membrane Protein 3Vesicular Transport ProteinsConceptsPre-vacuolar endosomesVacuolar proteinCarboxypeptidase Y pathwayDi-leucine sequenceOrganelle-specific functionsT-SNARE proteinsAP-3 functionAlkaline phosphatase pathwayYeast Saccharomyces cerevisiaeTransport of proteinsSNAP receptor (SNARE) proteinsT-SNAREsGenetic screenCPY pathwayLeucine signalDelivery of cargoPhosphatase pathwaysRegulated transportMammalian systemsPep12pVam3pSaccharomyces cerevisiaeAccessory proteinsAcid sequenceALP pathway
1997
A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.
Burd C, Peterson M, Cowles C, Emr S. A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Molecular Biology Of The Cell 1997, 8: 1089-1104. PMID: 9201718, PMCID: PMC305716, DOI: 10.1091/mbc.8.6.1089.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdenosine TriphosphatasesBiological TransportCell CompartmentationCytoskeletal ProteinsEndosomesFungal ProteinsGolgi ApparatusGTP-Binding ProteinsMacromolecular SubstancesMembrane FusionMembrane ProteinsProtein BindingQa-SNARE ProteinsRab GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStructure-Activity RelationshipVacuolesVesicular Transport ProteinsZincConceptsVacuolar protein sortingProtein sortingVPS pathwayEndosome transportSevere synthetic growth defectVacuolar protein sorting (VPS) genesVacuolar protein sorting (VPS) pathwaySynthetic growth defectsProtein sorting pathwaysZinc finger motifsRab family GTPaseCoiled-coil motifLocalization of proteinsSite-directed mutationsSDS-resistant complexesDominant negative mutationTsf phenotypeTsf mutationsFinger motifVesicle receptorsEndosome traffickingVacuole inheritanceVac1pVacuolar proteinTrans-GolgiNovel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component
Cowles C, Snyder W, Burd C, Emr S. Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component. The EMBO Journal 1997, 16: 2769-2782. PMID: 9184222, PMCID: PMC1169886, DOI: 10.1093/emboj/16.10.2769.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acid SequenceBiological TransportCarboxypeptidasesCarrier ProteinsCathepsin ACell CompartmentationFungal ProteinsGolgi ApparatusMembrane ProteinsModels, BiologicalMolecular Sequence DataNuclear ProteinsQa-SNARE ProteinsRecombinant Fusion ProteinsRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVacuolesVesicular Transport ProteinsConceptsVacuolar protein sorting (vps) mutantsSoluble vacuolar hydrolasesVacuolar membrane proteinSoluble vacuolar proteinsDomain-swapping experimentsNon-permissive conditionsNon-permissive temperatureCytoplasmic tail domainVacuolar deliveryT-SNAREsNovel GolgiTransport intermediatesSorting signalsVacuolar hydrolasesVesicle recognitionVacuolar proteinDouble mutantAmino acid portionVacuolar localizationMembrane proteinsTail domainDelivery pathwayPlasma membraneSelective packagingAlkaline phosphatase
1996
A Yeast Protein Related to a Mammalian Ras-Binding Protein, Vps9p, Is Required for Localization of Vacuolar Proteins
Burd C, Mustol P, Schu P, Emr S. A Yeast Protein Related to a Mammalian Ras-Binding Protein, Vps9p, Is Required for Localization of Vacuolar Proteins. Molecular And Cellular Biology 1996, 16: 2369-2377. PMID: 8628304, PMCID: PMC231225, DOI: 10.1128/mcb.16.5.2369.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceAnimalsCarrier ProteinsCloning, MolecularFungal ProteinsGenes, FungalGenetic Complementation TestGuanine Nucleotide Exchange FactorsHumansMammalsMolecular Sequence DataMutagenesisPolymerase Chain ReactionRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTemperatureVacuolesVesicular Transport ProteinsConceptsVacuolar protein sortingProtein sortingVacuolar proteinVPS pathwayVacuolar protein sorting (VPS) genesTemperature-sensitive growth defectTemperature-conditional alleleVacuolar protein precursorsFamily of proteinsSecretion of proteinsRab GTPaseRA-binding proteinsTransport vesiclesYeast proteinsHomology domainYeast SaccharomycesGrowth defectHuman proteinsVps9pDNA sequencesGene productsCytosolic proteinsNonpermissive temperatureCarboxypeptidase YIntracellular transport