2024
The sinking platform test: a novel paradigm to measure persistence in animal models
Floris G, Godar S, Braccagni G, Piras I, Ravens A, Zanda M, Huentelman M, Bortolato M. The sinking platform test: a novel paradigm to measure persistence in animal models. Neuropsychopharmacology 2024, 49: 1373-1382. PMID: 38396257, PMCID: PMC11251282, DOI: 10.1038/s41386-024-01827-0.Peer-Reviewed Original ResearchConceptsChronic administration of fluoxetinePrefrontal cortex of miceWeeks of social isolationAdministration of fluoxetineMeasure persistenceGoal-directed actionsSocially isolated miceMitigate depression riskCortex of micePlatform testVoluntary wheel runningChronic haloperidolPrefrontal cortexTemperamental traitsImipramine treatmentNeurobiological foundationsBehavioral tasksChronic stressChronic administrationImmediate-early genesFailure TrialWheel runningDepression riskAnimal modelsNovel interventions
2023
Orbitofrontal cortex microRNAs support long-lasting heroin seeking behavior in male rats
Zanda M, Floris G, Daws S. Orbitofrontal cortex microRNAs support long-lasting heroin seeking behavior in male rats. Translational Psychiatry 2023, 13: 117. PMID: 37031193, PMCID: PMC10082780, DOI: 10.1038/s41398-023-02423-4.Peer-Reviewed Original ResearchConceptsOpioid use disorderOrbitofrontal cortexHL animalsHeroin seekingDrug cravingSelf-administer heroinLong-term heroinMaintenance of abstinenceDrug seeking behaviorOpioid useHeroin cravingMale ratsPreclinical studiesUse disordersDrug useExposure paradigmBrain regionsAbstinence periodAbstinenceHeroinDrug cuesSeeking behaviourLow dosageCravingProteomic analysis
2022
Heroin Regulates Orbitofrontal Circular RNAs
Floris G, Gillespie A, Zanda M, Dabrowski K, Sillivan S. Heroin Regulates Orbitofrontal Circular RNAs. International Journal Of Molecular Sciences 2022, 23: 1453. PMID: 35163373, PMCID: PMC8836038, DOI: 10.3390/ijms23031453.Peer-Reviewed Original ResearchConceptsHeroin self-administrationOrbitofrontal cortexOpioid use disorderNeurobiological adaptationsSelf-administrationSelf-administer sucroseChronic heroin exposureRat orbitofrontal cortexOpioid-seeking behaviorTreat opioid use disorderHeroin exposureUse disorderBrain regionsOpioid heroinBehavioral responsesMale ratsFemale ratsHeroinSeeking behaviorDrug overdose deathsRatsOverdose deathsOpioidRNA splice variantsCortex
2021
Drug-associated cues and drug dosage contribute to increased opioid seeking after abstinence
Zanda M, Floris G, Daws S. Drug-associated cues and drug dosage contribute to increased opioid seeking after abstinence. Scientific Reports 2021, 11: 14825. PMID: 34290298, PMCID: PMC8295307, DOI: 10.1038/s41598-021-94214-4.Peer-Reviewed Original ResearchConceptsDrug cuesDrug seekingIncubation of heroin cravingIncubation of opioid cravingDrug self-administration paradigmLow dose of heroinDrug-associated cuesSelf-administration paradigmDoses of heroinDays of abstinenceDrug-taking patternsDrug intake historyHeroin seekingRate of relapseHeroin cravingAbstinence phaseSelf-administrationOpioid cravingOpioid use disorderPromote relapseAbstinence lengthOpioid seekingUse disorderCompletion of rehabilitation programsIntake history
2020
Analysis of Opioid-Seeking Behavior Through the Intravenous Self-Administration Reinstatement Model in Rats
Fattore L, Fadda P, Zanda M, Fratta W. Analysis of Opioid-Seeking Behavior Through the Intravenous Self-Administration Reinstatement Model in Rats. Methods In Molecular Biology 2020, 2201: 231-245. PMID: 32975804, DOI: 10.1007/978-1-0716-0884-5_21.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, IntravenousAnalgesics, OpioidAnimalsBehavior ControlBehavior, AddictiveConditioning, OperantCuesDisease Models, AnimalDrug-Seeking BehaviorHeroinInfusions, IntravenousMaleOpioid-Related DisordersRatsRats, Sprague-DawleyRats, WistarReinforcement, PsychologyRewardSelf AdministrationSubstance-Related DisordersConceptsNeurobiological mechanismsAnimal model of relapseExtinction-reinstatement modelHeroin-seeking behaviorReinstatement animal modelRelapse-like behaviorModel of relapseRelapse to heroinVulnerability to relapseNose poke responsesPanel press responseNonhuman primatesReinstatement modelDrug seekingLever pressOperant respondingDrug abstinenceAddiction researchVulnerability factorsPress responseSubstance useOperant responseAnimal modelsIndividual slipsSelf-administered
2018
The novel psychoactive substance methoxetamine induces persistent behavioral abnormalities and neurotoxicity in rats
Costa G, Serra M, Pintori N, Casu M, Zanda M, Murtas D, De Luca M, Simola N, Fattore L. The novel psychoactive substance methoxetamine induces persistent behavioral abnormalities and neurotoxicity in rats. Neuropharmacology 2018, 144: 219-232. PMID: 30366005, DOI: 10.1016/j.neuropharm.2018.10.031.Peer-Reviewed Original ResearchConceptsEffects of methoxetamineElevated plus mazePersistent behavioral abnormalitiesBehavioral effectsPlus mazeLocomotor activityBehavioral abnormalitiesDensity of dopamine transportersLocomotor activity of ratsImpaired non-spatial memoryNovel object recognition testEmission of ultrasonic vocalizationsMedial prefrontal cortexMarble burying testVentral tegmental areaPersistent behavioral effectsNon-spatial memoryNovel object recognitionObject recognition testActivity of ratsNeurotoxic effectsSubstantia nigra pars compactaSerotonergic damageNucleus accumbensBurying testOld and new synthetic cannabinoids: lessons from animal models
Zanda M, Fattore L. Old and new synthetic cannabinoids: lessons from animal models. Drug Metabolism Reviews 2018, 50: 54-64. PMID: 29376443, DOI: 10.1080/03602532.2018.1430824.Peer-Reviewed Original ResearchConceptsSynthetic cannabinoidsCannabinoid agonistsAnimal models of drug addictionConditioned place preference procedureModels of drug addictionPlace preference procedureBrain reward circuitsPositive reinforcing effectsDrug-induced behaviorsSynthetic cannabinoid agonistDelta-9-tetrahydrocannabinolReinstatement modelDrug discriminationReward circuitPreference procedureSelf-administrationAbuse liabilityPsychoactive componentAbuse potentialDrug addictionPsychiatric consequencesBehavioral studiesReinforcing effectsCannabinoidPsychoactive substances
2017
Psychedelics and reconsolidation of traumatic and appetitive maladaptive memories: focus on cannabinoids and ketamine
Fattore L, Piva A, Zanda M, Fumagalli G, Chiamulera C. Psychedelics and reconsolidation of traumatic and appetitive maladaptive memories: focus on cannabinoids and ketamine. Psychopharmacology 2017, 235: 433-445. PMID: 29178010, DOI: 10.1007/s00213-017-4793-4.Peer-Reviewed Original ResearchConceptsPost-traumatic stress disorderSubstance use disordersMaladaptive memoriesPsychedelic effectsReconsolidation of maladaptive memoriesAppetitive memory reconsolidationMemory reconsolidationMDMA effectsMemory inhibitionReconsolidation processStress disorderKetamine effectsPsychedelic substancesBiobehavioral processesReconsolidationCannabinoidMDMASubstance effectsMemoryPsychedelicsDisordersKetamineClasses of drugsPotential therapeutic effectsPotential therapeutic useMethoxetamine affects brain processing involved in emotional response in rats
Zanda M, Fadda P, Antinori S, Di Chio M, Fratta W, Chiamulera C, Fattore L. Methoxetamine affects brain processing involved in emotional response in rats. British Journal Of Pharmacology 2017, 174: 3333-3345. PMID: 28718892, PMCID: PMC5595753, DOI: 10.1111/bph.13952.Peer-Reviewed Original ResearchConceptsBehaviorally active dosesDecreased social interaction timeMotor activityMedial prefrontal cortexObsessive-compulsive traitsReduced immobility timeSocial interaction timeSelf-grooming behaviorIncreased phosphorylation of ribosomal protein S6Antidepressant effectsImmobility timePrefrontal cortexBehavioral effectsIncreased sociabilitySpatial anxietyAcute administrationBrain processesHigh dosesIncreased swimming activityEmotional responsesDissociative effectsEmotional statesIntermediate dosePsychoactive substancesMethoxetamine
2016
Methoxetamine, a novel psychoactive substance with serious adverse pharmacological effects
Zanda M, Fadda P, Chiamulera C, Fratta W, Fattore L. Methoxetamine, a novel psychoactive substance with serious adverse pharmacological effects. Behavioural Pharmacology 2016, 27: 489-496. PMID: 27128862, DOI: 10.1097/fbp.0000000000000241.Peer-Reviewed Original ResearchConceptsEffects of methoxetamineN-methyl-d-aspartate receptor antagonistic propertiesPsychoactive substancesReceptor antagonist propertiesOccurrence of adverse effectsAdverse pharmacological effectsDopamine neurotransmissionMesolimbic pathwayAntidepressant activityPreclinical dataPreclinical findingsAntagonistic propertiesLong-term effectsPharmacological effectsStimulatory effectMethoxetamineAdverse effectsKetamineLegal highsResearch chemicalsToxicological effectsPhencyclidineDopamineNeurotransmissionIncreasing number
2014
Analysis of Opioid-Seeking Reinstatement in the Rat
Fattore L, Fadda P, Zanda M, Fratta W. Analysis of Opioid-Seeking Reinstatement in the Rat. Methods In Molecular Biology 2014, 1230: 295-307. PMID: 25293336, DOI: 10.1007/978-1-4939-1708-2_25.Peer-Reviewed Original ResearchConceptsNeurobiological mechanismsAnimal model of relapseReinstatement animal modelRelapse-like behaviorHeroin-seeking behaviorRelapse to heroinVulnerability to relapseModel of relapseNose poke responsesPanel press responseNonhuman primatesReinstatement modelDrug seekingLever pressOperant respondingDrug abstinenceAddiction researchPress responseVulnerability factorsConditioned behaviorReinstatementSubstance useOperant responseAnimal modelsIndividual slips