2017
Mass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol
Hettick JM, Law BF, Lin CC, Wisnewski AV, Siegel PD. Mass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol. Xenobiotica 2017, 48: 626-636. PMID: 28629263, PMCID: PMC5864557, DOI: 10.1080/00498254.2017.1344791.Peer-Reviewed Original ResearchConceptsLavage fluidOccupational allergic respiratory diseaseBALB/c mouse modelMurine bronchoalveolar lavage fluidDiisocyanate-induced asthmaBronchoalveolar lavage fluidAllergic respiratory diseasesBronchoalveolar lavage samplesDiphenyl diisocyanate aerosolsLabel-free quantitative proteomic strategyMDI conjugationWestern blot analysisHealth burdenLavage samplesRespiratory diseaseAerosol exposureMouse modelDisease pathogenesisRespiratory exposureCausative agentBlot analysisCytochrome P450Argininosuccinate synthaseQuantitative proteomic strategyCellular fractions
2016
The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition
Hagerman LM, Law BF, Bledsoe TA, Hettick JM, Kashon ML, Lemons AR, Wisnewski AV, Siegel PD. The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition. Journal Of Occupational And Environmental Hygiene 2016, 13: 829-839. PMID: 27124286, PMCID: PMC5016257, DOI: 10.1080/15459624.2016.1183013.Peer-Reviewed Original ResearchConceptsOccupational asthmaDifferent conjugatesPolyclonal antibody responsePanel of antigensSpecific antibody clonesHuman serumMDI conjugationPolyclonal serum antibodiesSpecific IgEAntibody responseSerum antibodiesSerologic detectionStandard antigenMonoclonal antibodiesAntibody clonesAntibodiesAsthmaSignificant differencesAntigenSerumMAbsAntibody recognitionAntigenicityMinimal differencesConjugates
2015
Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation
Wisnewski AV, Liu J, Colangelo CM. Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation. Chemical Research In Toxicology 2015, 28: 729-737. PMID: 25635619, PMCID: PMC4667722, DOI: 10.1021/tx5005002.Peer-Reviewed Original ResearchConceptsEosinophilic inflammatory responseAlternative macrophage activationInflammatory responseChemical allergensMacrophage activationAirway eosinophil numbersEosinophilic airway inflammationInnate immune responseGreater inflammatory responseAirway levelsMDI conjugationAirway inflammationOccupational asthmaIL-12MDI exposureAsthma pathogenesisEosinophil numbersNaïve miceAirway fluidRespiratory tractImmune responseMucus productionSkin exposureMDI conjugatesVivo evidence
2011
Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses
Wisnewski AV, Xu L, Robinson E, Liu J, Redlich CA, Herrick CA. Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses. Journal Of Occupational Medicine And Toxicology 2011, 6: 6. PMID: 21414210, PMCID: PMC3068988, DOI: 10.1186/1745-6673-6-6.Peer-Reviewed Original ResearchRespiratory tract inflammationImmune sensitizationSkin exposureMDI conjugationBronchoalveolar lavageInflammatory responseRespiratory tract exposureAirway inflammatory responseAcetone/olive oilSubsequent immune responseSpecific antibody productionDisease prevention effortsAlbumin conjugatesOccupational asthmaSerum levelsMDI exposureAsthma pathogenesisSerum IgGIntranasal deliveryImmune responseImmune sensitivityMurine modelPathogenic mechanismsInflammationAntibody production
2010
Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate
Wisnewski AV, Liu J, Redlich CA. Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate. Analytical Biochemistry 2010, 400: 251-258. PMID: 20123080, PMCID: PMC3106273, DOI: 10.1016/j.ab.2010.01.037.Peer-Reviewed Original ResearchConceptsHigh-performance liquid chromatographyDiphenylmethane diisocyanateConjugation sitesReaction productsCross-linking agentTandem mass spectrometryPolyurethane productionMass spectrometryMDI-exposed workersCause of asthmaHuman albuminSerum immunoglobulin GLiquid chromatographyReaction specificityDiisocyanateSecond lysineMDI conjugationAirway inflammationAnalytical methodSelf proteinsProteomic methodologiesEpitope specificityAntigenic changesClinical researchConjugates