Bioinformatics workshop series
Session 4: RNAseq Data Processing: A Step-by-Step Guide from raw sequences to counts
Register: https://schedule.yale.edu/event/11967121
Presenter: Dr. Francesc Lopez-Giraldez, Yale Center for Genomic Analysis
Summary: Learn the detailed process of transforming raw RNAseq data (fastq format) into meaningful raw counts, without relying on automated pipelines. This hands-on workshop emphasizes the step-by-step methodology for data processing and analysis. For this hands-on workshop, participants will be using Open OnDemand and Jupyter Notebooks.
You will learn how to:
- QC, filter, and trim NGS sequences
- Align sequences to a reference genome
- Produce a count matrix ready to be used with DESeq2
Requirements:
- Have participated to session 3 or being familiar with bash/CLI or Jupyter Notebook
- Bring a laptop
- HPC account to access Yale Open OnDemand, if you don't have an account bring your laptop with R and RStudio installed.
This session is suitable for both beginner and intermediate users.
Speaker
Admission
Free
Tag
Training