2024
313 BEST4+ CFTR high expresser cells in normal rat are neuropods that sense and respond to luminal pH and are altered in dF508 CF intestine
Jin J, dos Reis D, Muiler C, Zagoren E, Donnelley M, Parsons D, Sumigray K, Ameen N. 313 BEST4+ CFTR high expresser cells in normal rat are neuropods that sense and respond to luminal pH and are altered in dF508 CF intestine. Journal Of Cystic Fibrosis 2024, 23: s167. DOI: 10.1016/s1569-1993(24)01153-6.Peer-Reviewed Original Research354 Elucidating the role of signaling and stress on CFTR and its contribution to diarrhea in SGK1 knockout mouse intestine
Muiler C, Santos A, Dastoor P, Jin J, dos Reis D, Hoque K, Ameen N. 354 Elucidating the role of signaling and stress on CFTR and its contribution to diarrhea in SGK1 knockout mouse intestine. Journal Of Cystic Fibrosis 2024, 23: s185. DOI: 10.1016/s1569-1993(24)01194-9.Peer-Reviewed Original ResearchLocalization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta
Dastoor P, Muiler C, Garrison A, Egan M, Carlos Dos Reis D, Santos A, Ameen N. Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta. European Journal Of Pharmacology 2024, 978: 176771. PMID: 38925289, DOI: 10.1016/j.ejphar.2024.176771.Peer-Reviewed Original ResearchSerum glucocorticoid kinase 1Glucocorticoid kinase 1F508del-CFTRCystic fibrosisMouse modelF508del-CFTR miceCFTR-expressing epithelial cellsCF mouse modelsHumanized mouse modelTreatment of ratsIntestinal diseaseIntestinal segmentsKinase 1CFTR modulatorsCFTR mutationsCF patientsTrikaftaDex treatmentLung diseaseEfficacy of compoundsDelta F508CFTRCombined treatmentEpithelial cellsLoss of functionSu1930 STRESS-INDUCED SIGNALING AND CFTR IN SGK1 KNOCK OUT MOUSE INTESTINE
Muiler C, Santos A, Dastoor P, Jin J, Reis D, Kazi M, Ameen N. Su1930 STRESS-INDUCED SIGNALING AND CFTR IN SGK1 KNOCK OUT MOUSE INTESTINE. Gastroenterology 2024, 166: s-874. DOI: 10.1016/s0016-5085(24)02489-2.Peer-Reviewed Original Research978 CFTR HIGH EXPRESSER CELLS EXPRESS NEUROPOD GENES AND RESPOND TO ENVIRONMENTAL CUES IN THE INTESTINE
Jin J, Reis D, Muiler C, Sumigray K, Ameen N. 978 CFTR HIGH EXPRESSER CELLS EXPRESS NEUROPOD GENES AND RESPOND TO ENVIRONMENTAL CUES IN THE INTESTINE. Gastroenterology 2024, 166: s-236. DOI: 10.1016/s0016-5085(24)01027-8.Peer-Reviewed Original Research
2023
555 Elexacaftor-tezacaftor-ivacaftor and serum glucocorticoid kinase 1 activation on humanized dF508 mouse intestine
Ameen N, Dastoor P, dos Reis D, Barone C, Garrison A, Egan M. 555 Elexacaftor-tezacaftor-ivacaftor and serum glucocorticoid kinase 1 activation on humanized dF508 mouse intestine. Journal Of Cystic Fibrosis 2023, 22: s293-s294. DOI: 10.1016/s1569-1993(23)01478-9.Peer-Reviewed Original Research539 Role of CFTR high expresser cells in cystic fibrosis intestinal disease
Ameen N, dos Reis D, Dastoor P, Santos A, Cohen M, Donnelley M, Parsons D. 539 Role of CFTR high expresser cells in cystic fibrosis intestinal disease. Journal Of Cystic Fibrosis 2023, 22: s285. DOI: 10.1016/s1569-1993(23)01462-5.Peer-Reviewed Original ResearchCFTR High Expresser Cells in cystic fibrosis and intestinal diseases
dos Reis D, Dastoor P, Santos A, Sumigray K, Ameen N. CFTR High Expresser Cells in cystic fibrosis and intestinal diseases. Heliyon 2023, 9: e14568. PMID: 36967909, PMCID: PMC10031467, DOI: 10.1016/j.heliyon.2023.e14568.Peer-Reviewed Original ResearchCystic fibrosisIntestinal diseaseCystic fibrosis transmembrane conductance regulatorQuality of lifeIntestinal dysfunctionGastrointestinal diseasesIntestinal physiologyFibrosisDiseasePathophysiologyFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCFTR leadConductance regulator
2022
Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal Of Clinical Medicine 2022, 11: 4179. PMID: 35887942, PMCID: PMC9319011, DOI: 10.3390/jcm11144179.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseSevere diarrheaInclusion diseaseFluid secretionCystic fibrosis transmembrane conductance regulatorDouble knockout miceMicrovillus inclusionsGlucocorticoid-inducible kinase 1Carbohydrate malabsorptionKnockout miceIntestinal carbohydrateDiarrheaDiarrheal diseaseMalabsorptionMiceVillus enterocytesDiseaseLoss of MYO5BFunction mutationsApical cystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorKinase 1SecretionIntestineTransmembrane conductance regulatorElevated 5‐hydroxytryptamine in COVID‐19 Stimulates ANO1 Mediated Cl Secretion in Lung & Intestinal Epithelial Cells
Hoque K, Hayashi M, Sheikh I, Banerjee A, Verma S, Leblanc N, Zeiss C, Ameen N, Chakraborty S. Elevated 5‐hydroxytryptamine in COVID‐19 Stimulates ANO1 Mediated Cl Secretion in Lung & Intestinal Epithelial Cells. The FASEB Journal 2022, 36: 10.1096/fasebj.2022.36.s1.0r556. PMCID: PMC9348250, DOI: 10.1096/fasebj.2022.36.s1.0r556.Peer-Reviewed Original ResearchEnterochromaffin cell hyperplasiaCalu-3 cellsSARS-CoV2 infectionCl(-) secretionIntestinal epitheliumCell hyperplasiaColonic tissueCalcium-activated Cl- currentsCOVID-19-associated diarrheaCalcium-activated chloride currentsHuman colorectal cancer tissuesANO1 protein expressionCOVID-19 therapyIntestinal Cl(-) secretionColorectal cancer tissuesUssing chamber experimentsANO1 currentsSeverity of symptomsCOVID-19Human terminal ileumHuman intestinal epitheliumIntestinal epithelial cellsWhole-cell currentsHuman colonic tissueCaco-2 cellsSerum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis D, Barbieri A, Sumigray K, Nottoli T, Salas P, Ameen N. Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.r5730.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseGlucocorticoid-inducible kinase 1Severe diarrheaF miceInclusion diseaseCystic fibrosis transmembrane conductance regulatorFluid secretionWorsening of diarrheaMicrovillus inclusionsPhosphorylation of PDK1Kinase 1Intestinal lysatesSGK1 pathwayCarbohydrate malabsorptionER miceTamoxifen inductionBeta-ENaCDiarrheaDiarrheal diseaseSmall intestineMalabsorptionMiceDCKO miceGlucose lossPhosphorylation of SGK1
2021
Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner
Saha T, Aoun J, Hayashi M, Ali I, Sarkar P, Bag PK, Leblanc N, Ameen N, Woodward OM, Hoque KM. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner. Biochemistry And Biophysics Reports 2021, 25: 100912. PMID: 33537462, PMCID: PMC7838733, DOI: 10.1016/j.bbrep.2021.100912.Peer-Reviewed Original Research
2020
Secretory Diarrhea
Ameen N, Kopic S, Ahsan K, Figueroa-Hall L. Secretory Diarrhea. Physiology In Health And Disease 2020, 41-76. DOI: 10.1007/978-3-030-55310-4_2.Peer-Reviewed Original ResearchIntestinal TMEM16A function as a luminal chloride channel
Kazi M, Saha T, Aoun J, Hayashi M, Sheikh I, Leblanc N, Sarkar P, Ameen N, Woodward O. Intestinal TMEM16A function as a luminal chloride channel. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.06115.Peer-Reviewed Original ResearchMouse intestinal tissueIntestinal tissueHuman colonic tissueColonic tissueLuminal expressionLuminal ClTMEM16A protein expressionIntestinal epithelial tissueAvailable polyclonal antibodyLuminal membraneWestern blot analysisTMEM16A functionSerosal applicationAgonist carbacholDistal colonCarbacholMRNA expressionColonWestern blotMouse intestineT84 cellsHigh expressionHuman colonProtein expressionTMEM16A protein
2019
36 – Glucocorticoids and Sgk1- Potent Regulators of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in the Intestine
Figueroa-Hall L, Ahsan K, Ahmed M, Kazi M, Ameen N. 36 – Glucocorticoids and Sgk1- Potent Regulators of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in the Intestine. Gastroenterology 2019, 156: s-14. DOI: 10.1016/s0016-5085(19)36808-8.Peer-Reviewed Original Research
2018
907 - Deficits in Apical Sodium and Water Transporters Along with Maintenance of CFTR Account for Diarrheal Pathology in MYO5B Ko Mice and Patients with MVID
Engevik A, Engevik M, Meyer A, Shub M, Koepsell H, Ameen N, Tyska M, Goldenring J. 907 - Deficits in Apical Sodium and Water Transporters Along with Maintenance of CFTR Account for Diarrheal Pathology in MYO5B Ko Mice and Patients with MVID. Gastroenterology 2018, 154: s-179. DOI: 10.1016/s0016-5085(18)31009-6.Peer-Reviewed Original ResearchEstablishment of Microvillus Inclusion Disease (MVID) human models using small intestinal enteroids and embryonic stem cells
Figueroa‐Hall L, Ameen N. Establishment of Microvillus Inclusion Disease (MVID) human models using small intestinal enteroids and embryonic stem cells. The FASEB Journal 2018, 32: 750.31-750.31. DOI: 10.1096/fasebj.2018.32.1_supplement.750.31.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseHuman intestinal modelSmall intestineIntestinal modelSeverity of illnessIntestinal organoidsSmall intestinal modelProximal small intestineCancer-derived cell linesFull-text articlesDevelopment of therapiesStem cellsIntestinal cell modelSmall intestinal organoidsIntestinal enteroidsInclusion diseasePathognomonic featuresSecretory diarrheaEpithelial diseaseHuman embryonic stem cellsIntestinal tissueIntestine resultsTransporter traffickingAppropriate research modelsLentiviral transfection
2017
Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine
Ahsan K, Tchernychev B, Kessler MM, Solinga RM, Arthur D, Linde CI, Silos‐Santiago I, Hannig G, Ameen NA. Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine. Physiological Reports 2017, 5: e13299. PMID: 28592587, PMCID: PMC5471438, DOI: 10.14814/phy2.13299.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Line, TumorCell MembraneCyclic AMP-Dependent Protein KinasesCyclic GMPCyclic GMP-Dependent Protein Kinase Type IICystic Fibrosis Transmembrane Conductance RegulatorGuanylyl Cyclase C AgonistsHumansIntestinal MucosaMalePeptidesProtein TransportRatsRats, Sprague-DawleyReceptors, Guanylate Cyclase-CoupledSignal TransductionConceptsRat intestinal loopsLinaclotide treatmentFluid secretionIntestinal loopsCystic fibrosis transmembrane conductance regulatorCell surfaceFibrosis transmembrane conductance regulatorCell surface traffickingChronic idiopathic constipationIrritable bowel syndromeTransmembrane conductance regulatorIntestinal fluid secretionCell surface translocationReceptor guanylyl cyclaseHuman intestinal tissueCaco-2BBe cellsCFTR traffickingCFTR pathwaySubapical compartmentCellular signalingCGMP/PKGProtein kinaseSurface biotinylationIdiopathic constipationPathway componentsAP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine
Kumari V, Desai S, Ameen NA. AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine. Journal Of Cystic Fibrosis 2017, 16: 327-334. PMID: 28438500, PMCID: PMC5502754, DOI: 10.1016/j.jcf.2017.03.012.Peer-Reviewed Original Research986 Deficits in Enterocyte Apical Transporters Associated with Loss of Myosin VB
Engevik A, Weis V, Knowles B, Schlegel C, Ameen N, Koepsell H, Zachos N, Donowitz M, Goldenring J. 986 Deficits in Enterocyte Apical Transporters Associated with Loss of Myosin VB. Gastroenterology 2017, 152: s187. DOI: 10.1016/s0016-5085(17)30935-6.Peer-Reviewed Original Research