2024
Intravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients
Yamaki T, Oka S, Iyama S, Sasaki M, Onodera R, Kataoka-Sasaki Y, Namioka T, Namioka A, Nakazaki M, Takemura M, Ukai R, Yokoyama T, Sasaki Y, Yamashita T, Kobayashi M, Yamaguchi M, Fukino M, Takazawa T, Hayasaka M, Owaku T, Funakura M, Onodera S, Ito Y, Kobune M, Kato J, Ishiai S, Kocsis J, Odaki M, Iwadate Y, Kobayashi S, Honmou O. Intravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients. Interdisciplinary Neurosurgery 2024, 36: 101927. DOI: 10.1016/j.inat.2023.101927.Peer-Reviewed Original ResearchSevere brain injury patientsAutologous mesenchymal stem cellsBrain injury patientsIntravenous infusionMesenchymal stem cellsAdverse eventsInjury patientsInfused mesenchymal stem cellsHealth Stroke ScaleSerious adverse eventsFugl-Meyer AssessmentInitial case seriesBrain metabolic activityStem cellsMSC infusionStroke ScaleNeurological deteriorationBarthel IndexCase seriesCNS tumorsNeurological functionFunctional statusFunctional improvementChronic patientsPlacebo effectA surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats
Yasuda N, Sasaki M, Kocsis J, Kawaharada N, Honmou O. A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats. World Neurosurgery 2024, 188: e349-e356. PMID: 38789035, DOI: 10.1016/j.wneu.2024.05.114.Peer-Reviewed Original ResearchIschemic spinal cord injurySpinal cord ischemiaCord ischemiaComplication rateRat modelTherapeutic strategiesEvaluate new therapeutic strategiesFunctional recoveryMale Sprague-Dawley ratsLow complication rateSprague-Dawley ratsSpecialized surgical equipmentExperimental animal modelsImprove functional recoveryPromote functional recoverySpinal cord injuryCross-clampingSevere neurological disordersAzygos veinSurgical protocolDescending aortaBulldog clampsLumbar levelsSpinal cordIschemic lesions
2023
Rehabilitation facilitates functional improvement following intravenous infusion of mesenchymal stem cells in the chronic phase of cerebral ischemia in rats
Yamashita T, Sasaki M, Sasaki Y, Nagahama H, Oka S, Kataoka-Sasaki Y, Ukai R, Yokoyama T, Kobayashi M, Kakizawa M, Kocsis J, Honmou O. Rehabilitation facilitates functional improvement following intravenous infusion of mesenchymal stem cells in the chronic phase of cerebral ischemia in rats. Brain Research 2023, 1825: 148709. PMID: 38072373, DOI: 10.1016/j.brainres.2023.148709.Peer-Reviewed Original ResearchActin Alpha 2, Smooth Muscle (ACTA2) Is Involved in the Migratory Potential of Malignant Gliomas, and Its Increased Expression at Recurrence Is a Significant Adverse Prognostic Factor
Hoshimaru T, Nonoguchi N, Kosaka T, Furuse M, Kawabata S, Yagi R, Kurisu Y, Kashiwagi H, Kameda M, Takami T, Kataoka-Sasaki Y, Sasaki M, Honmou O, Hiramatsu R, Wanibuchi M. Actin Alpha 2, Smooth Muscle (ACTA2) Is Involved in the Migratory Potential of Malignant Gliomas, and Its Increased Expression at Recurrence Is a Significant Adverse Prognostic Factor. Brain Sciences 2023, 13: 1477. PMID: 37891844, PMCID: PMC10605410, DOI: 10.3390/brainsci13101477.Peer-Reviewed Original ResearchActin alpha 2Malignant gliomasDistant recurrenceACTA2 expressionKD groupSmooth muscleSignificant adverse prognostic factorMode of recurrenceAlpha 2Progression-free survivalAdverse prognostic factorHuman malignant glioma cell linesMalignant glioma cell linesClinical glioma samplesGlioma cell linesPrognostic factorsBrain invasionTumor recurrenceInvasive tumorsNovel therapiesGlioma patientsSame patientRecurrenceControl groupPotential biomarkersRepeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingTherapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administrationMesenchymal Stem Cell Transplantation for Spinal Cord Injury: Current Status and Prospects
Hirota R, Sasaki M, Honmou O, Yamashita T. Mesenchymal Stem Cell Transplantation for Spinal Cord Injury: Current Status and Prospects. Spine Surgery And Related Research 2023, 7: 319-326. PMID: 37636138, PMCID: PMC10447197, DOI: 10.22603/ssrr.2022-0234.Peer-Reviewed Original ResearchSpinal cord injuryMesenchymal stem cellsCord injuryMesenchymal stem cell transplantationSevere spinal cord injuryCentral nervous system diseaseMotor function recoveryStem cell transplantationNervous system diseasesSCI modelCell transplantationFunction recoveryIntravenous infusionMSC transplantationMotor functionRat modelIntravenous administrationSystem diseasesBone marrowSCI researchTransplantationInjuryRecent updatesStem cellsCell typesA practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats
Nagahama H, Sasaki M, Komatsu K, Sato K, Katagiri Y, Kamagata M, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Terada K, Kobayashi M, Kocsis J, Honmou O. A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats. Journal Of Neuroscience Methods 2023, 386: 109784. PMID: 36608904, DOI: 10.1016/j.jneumeth.2023.109784.Peer-Reviewed Original ResearchInvestigation of the therapeutic efficacy of bone marrow mesenchymal stem cells for neuropathic pain
Fukushi R, Sasaki M, Yamashita T, Honmou O. Investigation of the therapeutic efficacy of bone marrow mesenchymal stem cells for neuropathic pain. Brain And Spine 2023, 3: 102427. DOI: 10.1016/j.bas.2023.102427.Peer-Reviewed Original Research
2022
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model
Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S. Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model. Scientific Reports 2022, 12: 16986. PMID: 36216855, PMCID: PMC9551049, DOI: 10.1038/s41598-022-18485-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowDisease Models, AnimalGreen Fluorescent ProteinsHindlimbInfusions, IntravenousIschemiaLower ExtremityMesenchymal Stem Cell TransplantationMesenchymal Stem CellsNeovascularization, PhysiologicPerfusionPeripheral Vascular DiseasesRatsRats, Sprague-DawleyVascular Endothelial Growth Factor AConceptsIntravenous infusionVascular endothelial growth factorMesenchymal stem cellsTissue perfusionHindlimb ischemia modelRat hindlimb ischemia modelIschemia modelCell groupsFemoral artery removalIschemia animal modelStem cellsHindlimb muscle tissueBone marrow-derived mesenchymal stem cellsEndothelial growth factorMarrow-derived mesenchymal stem cellsMSC infusionHistological findingsIschemic limbsHindlimb ischemiaCell delivery methodsIschemic hindlimbSprague-DawleyCapillary densityAnimal modelsInfusionEnhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projectionsIntravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial
Oka S, Yamaki T, Sasaki M, Ukai R, Takemura M, Yokoyama T, Kataoka-Sasaki Y, Onodera R, Ito YM, Kobayashi S, Kocsis JD, Iwadate Y, Honmou O. Intravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial. JMIR Research Protocols 2022, 11: e37898. PMID: 35793128, PMCID: PMC9301565, DOI: 10.2196/37898.Peer-Reviewed Original ResearchChronic brain injuryAutologous mesenchymal stem cellsSpinal cord injuryINTERNATIONAL REGISTERED REPORT IDENTIFIERBrain injuryIntravenous infusionRankin Scale gradesCord injuryMesenchymal stem cellsClinical trialsScale gradeTherapeutic efficacyJapan Medical Association CenterSapporo Medical University HospitalInvestigator-initiated clinical trialsModified Rankin Scale gradesOpen-label trialPhase 2 studyPhase 2 trialMedical University HospitalCause of disabilityMotor vehicle accidentsPotential therapeutic efficacyProportion of casesStem cellsThe Impact of Purple-Flesh Potato (Solanum tuberosum L.) cv. “Shadow Queen” on Minor Health Complaints in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Study
Maeda-Yamamoto M, Honmou O, Sasaki M, Haseda A, Kagami-Katsuyama H, Shoji T, Namioka A, Namioka T, Magota H, Oka S, Kataoka-Sasaki Y, Ukai R, Takemura M, Nishihira J. The Impact of Purple-Flesh Potato (Solanum tuberosum L.) cv. “Shadow Queen” on Minor Health Complaints in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Study. Nutrients 2022, 14: 2446. PMID: 35745176, PMCID: PMC9227939, DOI: 10.3390/nu14122446.Peer-Reviewed Original ResearchConceptsMinor health complaintsHealth complaintsHealthy adultsMesenchymal stem cellsMSC countPlacebo groupWeeks 0Psychological stress responsesHealthy subjectsIntake periodStress responseAdverse effectsComplaintsTest groupStem cellsStudy determinesAdultsGroupCountPurple potatoesResponsePlaceboIrritabilityIntakeIngestionPharmacological Difference Between Platelet Aggregations in Cardioembolic Stroke Patients with Direct Oral Anticoagulants: A Pilot Study
Nakazaki M, Oka S, Magota H, Kiyose R, Onodera R, Ukai R, Kataoka-Sasaki Y, Sasaki M, Honmou O. Pharmacological Difference Between Platelet Aggregations in Cardioembolic Stroke Patients with Direct Oral Anticoagulants: A Pilot Study. Journal Of Stroke And Cerebrovascular Diseases 2022, 31: 106520. PMID: 35523052, DOI: 10.1016/j.jstrokecerebrovasdis.2022.106520.Peer-Reviewed Original ResearchConceptsDirect oral anticoagulantsPlatelet aggregation testCardioembolic strokePlatelet aggregationFXa inhibitorsOral anticoagulantsStroke patientsPharmacological differencesAdenosine diphosphateAggregation testConcurrent antiplatelet therapyIschemic stroke patientsCardioembolic stroke patientsOnset of symptomsResults Six patientsLight transmission aggregometryFactor Xa inhibitorsAntiplatelet therapySecondary preventionSix patientsAntiplatelet drugsAtrial fibrillationAntiplatelet effectXa inhibitorsDabigatran
2021
Repeated intravenous infusion of mesenchymal stem cells for enhanced functional recovery in a rat model of chronic cerebral ischemia.
Takemura M, Sasaki M, Kataoka-Sasaki Y, Kiyose R, Nagahama H, Oka S, Ukai R, Yokoyama T, Kocsis J, Ueba T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells for enhanced functional recovery in a rat model of chronic cerebral ischemia. Journal Of Neurosurgery 2021, 137: 402-411. PMID: 34861644, DOI: 10.3171/2021.8.jns21687.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionInduction of MCAOCorpus callosumMesenchymal stem cellsChronic phaseMotor functionRat modelWeek 8Vehicle groupInterhemispheric connectionsAdministration of MSCsChronic cerebral ischemia modelNeural connectionsChronic stroke modelCerebral artery occlusionChronic cerebral ischemiaIschemic lesion volumeLong-term disabilityGreater functional improvementCerebral ischemia modelEnhanced functional recoveryAnti-neurofilament antibodiesDiffusion tensorSingle systemic infusionStem cellsPossible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat
Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O. Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat. BMC Urology 2021, 21: 156. PMID: 34774029, PMCID: PMC8590770, DOI: 10.1186/s12894-021-00923-3.Peer-Reviewed Original ResearchConceptsHunner-type ICBladder pain syndromeAnti-inflammatory pathwayMSC groupMesenchymal stem cellsPain syndromeAnimal modelsToll-like receptor 7 agonistTherapeutic efficacyBehavior testsRight external jugular veinFemale Sprague-Dawley ratsGFP-positive mesenchymal stem cellsReceptor 7 agonistChronic pelvic painAnti-inflammatory effectsEfficacious treatment optionExperimental animal modelsSprague-Dawley ratsExternal jugular veinReal-time polymerase chain reactionMRNA expression levelsPositive mesenchymal stem cellsStem cellsHunner lesions3.神経再生治療によって惹起される神経可塑性の MRI による評価
Nagahama H, Sasaki M, Kiyose R, Yasuda N, Honmou O. 3.神経再生治療によって惹起される神経可塑性の MRI による評価. Japanese Journal Of Radiological Technology 2021, 77: 1238-1244. PMID: 34670933, DOI: 10.6009/jjrt.2021_jsrt_77.10.1238.Peer-Reviewed Original ResearchIntravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats
Nakagawa T, Sasaki M, Kataoka-Sasaki Y, Yotsuyanagi T, Radtke C, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats. Plastic & Reconstructive Surgery 2021, 148: 799-807. PMID: 34550936, DOI: 10.1097/prs.0000000000008327.Peer-Reviewed Original ResearchConceptsMesenchymal stem cell groupStem cell groupIntravenous infusionRandom pattern flapsCell groupsMesenchymal stem cellsFlap survivalHistologic analysisRandom-pattern skin flapsBlood perfusionSurgical reconstruction optionsHigh expressionSkin flap survivalFibroblast growth factor-2 (FGF-2) mRNAQuantitative reverse transcription polymerase chain reactionStem cellsFlap survival areaReverse transcription-polymerase chain reactionSkin flap surgeryExperimental disease modelsSkin blood perfusionSkin flap modelSoft tissue defectsMasson's trichrome stainingMasson's trichrome staining resultsRepeated infusion of mesenchymal stem cells maintain the condition to inhibit deteriorated motor function, leading to an extended lifespan in the SOD1G93A rat model of amyotrophic lateral sclerosis
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Repeated infusion of mesenchymal stem cells maintain the condition to inhibit deteriorated motor function, leading to an extended lifespan in the SOD1G93A rat model of amyotrophic lateral sclerosis. Molecular Brain 2021, 14: 76. PMID: 33962678, PMCID: PMC8103621, DOI: 10.1186/s13041-021-00787-6.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisAdministration of MSCsSingle infusionMesenchymal stem cellsMotor functionGait abilityDisease progressionMotor neuronsRat modelLateral sclerosisBlood-spinal cord barrierSOD1G93A rat modelSpinal cord degenerateVehicle infusion groupHind limb functionALS rat modelStem cellsLimb functionVehicle infusionFatal disorderSurvival periodTherapeutic effectLocomotor activityInfusionProgressive deteriorationIntravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia
Kiyose R, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Magota H, Oka S, Ukai R, Takemura M, Yokoyama T, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia. World Neurosurgery 2021, 149: e160-e169. PMID: 33618048, DOI: 10.1016/j.wneu.2021.02.056.Peer-Reviewed Original ResearchConceptsRegional cerebral blood flowInfused mesenchymal stem cellsReperfusion therapyPermanent MCAOIntravenous infusionMesenchymal stem cellsTherapeutic efficacyTransient middle cerebral artery occlusion modelMiddle cerebral artery occlusion modelArterial spin labeling magnetic resonance imagingArtery occlusion modelStandard therapeutic strategyTransient MCAO modelCerebral blood flowBehavioral functionsNon-favorable outcomeRat model systemStem cellsMCAO groupStroke inductionAcute strokeCerebral ischemiaNeurologic functionInfusion groupMCAO model