2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingTherapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administration
2022
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model
Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S. Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model. Scientific Reports 2022, 12: 16986. PMID: 36216855, PMCID: PMC9551049, DOI: 10.1038/s41598-022-18485-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowDisease Models, AnimalGreen Fluorescent ProteinsHindlimbInfusions, IntravenousIschemiaLower ExtremityMesenchymal Stem Cell TransplantationMesenchymal Stem CellsNeovascularization, PhysiologicPerfusionPeripheral Vascular DiseasesRatsRats, Sprague-DawleyVascular Endothelial Growth Factor AConceptsIntravenous infusionVascular endothelial growth factorMesenchymal stem cellsTissue perfusionHindlimb ischemia modelRat hindlimb ischemia modelIschemia modelCell groupsFemoral artery removalIschemia animal modelStem cellsHindlimb muscle tissueBone marrow-derived mesenchymal stem cellsEndothelial growth factorMarrow-derived mesenchymal stem cellsMSC infusionHistological findingsIschemic limbsHindlimb ischemiaCell delivery methodsIschemic hindlimbSprague-DawleyCapillary densityAnimal modelsInfusion
2021
Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat
Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O. Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat. BMC Urology 2021, 21: 156. PMID: 34774029, PMCID: PMC8590770, DOI: 10.1186/s12894-021-00923-3.Peer-Reviewed Original ResearchConceptsHunner-type ICBladder pain syndromeAnti-inflammatory pathwayMSC groupMesenchymal stem cellsPain syndromeAnimal modelsToll-like receptor 7 agonistTherapeutic efficacyBehavior testsRight external jugular veinFemale Sprague-Dawley ratsGFP-positive mesenchymal stem cellsReceptor 7 agonistChronic pelvic painAnti-inflammatory effectsEfficacious treatment optionExperimental animal modelsSprague-Dawley ratsExternal jugular veinReal-time polymerase chain reactionMRNA expression levelsPositive mesenchymal stem cellsStem cellsHunner lesionsIntravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats
Nakagawa T, Sasaki M, Kataoka-Sasaki Y, Yotsuyanagi T, Radtke C, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Promotes the Survival of Random Pattern Flaps in Rats. Plastic & Reconstructive Surgery 2021, 148: 799-807. PMID: 34550936, DOI: 10.1097/prs.0000000000008327.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalGraft SurvivalHumansInfusions, IntravenousMaleMesenchymal Stem Cell TransplantationMesenchymal Stem CellsRatsSurgical FlapsConceptsMesenchymal stem cell groupStem cell groupIntravenous infusionRandom pattern flapsCell groupsMesenchymal stem cellsFlap survivalHistologic analysisRandom-pattern skin flapsBlood perfusionSurgical reconstruction optionsHigh expressionSkin flap survivalFibroblast growth factor-2 (FGF-2) mRNAQuantitative reverse transcription polymerase chain reactionStem cellsFlap survival areaReverse transcription-polymerase chain reactionSkin flap surgeryExperimental disease modelsSkin blood perfusionSkin flap modelSoft tissue defectsMasson's trichrome stainingMasson's trichrome staining resultsIntravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia
Kiyose R, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Magota H, Oka S, Ukai R, Takemura M, Yokoyama T, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia. World Neurosurgery 2021, 149: e160-e169. PMID: 33618048, DOI: 10.1016/j.wneu.2021.02.056.Peer-Reviewed Original ResearchConceptsRegional cerebral blood flowInfused mesenchymal stem cellsReperfusion therapyPermanent MCAOIntravenous infusionMesenchymal stem cellsTherapeutic efficacyTransient middle cerebral artery occlusion modelMiddle cerebral artery occlusion modelArterial spin labeling magnetic resonance imagingArtery occlusion modelStandard therapeutic strategyTransient MCAO modelCerebral blood flowBehavioral functionsNon-favorable outcomeRat model systemStem cellsMCAO groupStroke inductionAcute strokeCerebral ischemiaNeurologic functionInfusion groupMCAO modelIntravenous infusion of auto serum-expanded autologous mesenchymal stem cells in spinal cord injury patients: 13 case series
Honmou O, Yamashita T, Morita T, Oshigiri T, Hirota R, Iyama S, Kato J, Sasaki Y, Ishiai S, Ito YM, Namioka A, Namioka T, Nakazaki M, Kataoka-Sasaki Y, Onodera R, Oka S, Sasaki M, Waxman SG, Kocsis JD. Intravenous infusion of auto serum-expanded autologous mesenchymal stem cells in spinal cord injury patients: 13 case series. Clinical Neurology And Neurosurgery 2021, 203: 106565. PMID: 33667953, DOI: 10.1016/j.clineuro.2021.106565.Peer-Reviewed Original ResearchConceptsSpinal cord injuryAmerican Spinal Injury Association Impairment ScaleMSC infusionAutologous mesenchymal stem cellsASIA CASIA DIntravenous infusionMesenchymal stem cellsSCI patientsFunctional statusFunctional improvementSpinal cord injury patientsSpinal Cord Independence MeasureASIA B patientsASIA C patientsPhase 2 studySerious adverse eventsCord injury patientsCurrent therapeutic optionsStem cellsASIA BASIA gradeC patientsNeurologic improvementAdverse eventsIntravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model. Brain Research 2021, 1757: 147296. PMID: 33516815, DOI: 10.1016/j.brainres.2021.147296.Peer-Reviewed Original ResearchConceptsBlood-spinal cord barrierQuantitative reverse transcription polymerase chain reactionIntravenous infusionDisease progressionMotor neuronsMSC groupLocomotor functionOpen-field locomotor functionPreservation of microvasculatureHind limb functionCommon clinical featuresEvans blue leakageMotor neuron lossReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionDevastating neurodegenerative diseaseBBB scoringBSCB functionRotarod testingClinical featuresNeuron lossLimb functionNeurotrophic factorCurative strategiesSpinal cord
2019
Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model
Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Suzuki J, Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model. Neuroscience 2019, 408: 361-377. PMID: 30999031, DOI: 10.1016/j.neuroscience.2019.04.018.Peer-Reviewed Original ResearchConceptsCerebral small vessel diseaseBlood-brain barrierProgressive brain atrophyVascular dementiaMesenchymal stem cellsAlzheimer's diseaseCognitive functionBrain atrophyIntravenous infusionEffects of MSCsInfused mesenchymal stem cellsSmall vessel diseaseEssential pathological featuresAccumulation of AβSpinal cord injuryBBB transport systemsStem cellsBBB functionAβ accumulationHypertensive ratsNeuronal damageFunctional recoveryVessel diseaseCord injuryPathological features
2018
Preservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction
Nagahama H, Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Namioka T, Namioka A, Oka S, Onodera R, Suzuki J, Sasaki Y, Kocsis JD, Honmou O. Preservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction. Brain Research 2018, 1695: 37-44. PMID: 29802840, DOI: 10.1016/j.brainres.2018.05.033.Peer-Reviewed Original ResearchConceptsInterhemispheric cortical connectionsCorpus callosumIntravenous infusionMesenchymal stem cellsDiffusion tensor imagingMotor cortexFunctional improvementCortical connectionsAdeno-associated virusRat middle cerebral artery occlusion stroke modelMiddle cerebral artery occlusion stroke modelContralateral motor cortexBlood-brain barrierMotor cortex connectivityVehicle-infused groupMRI diffusion tensor imagingPotential therapeutic mechanismNeuroanatomical tracing techniquesStem cellsCerebral infarctionInflammatory infiltrationAnatomical restorationCerebral strokeFunctional recoveryNeurotrophic factorIntravenous infusion of mesenchymal stem cells reduces epileptogenesis in a rat model of status epilepticus
Fukumura S, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Nagahama H, Morita T, Sakai T, Tsutsumi H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells reduces epileptogenesis in a rat model of status epilepticus. Epilepsy Research 2018, 141: 56-63. PMID: 29475054, DOI: 10.1016/j.eplepsyres.2018.02.008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalGlutamate DecarboxylaseGreen Fluorescent ProteinsHippocampusInfusions, IntravenousLithiumMagnetic Resonance ImagingMaleMaze LearningMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMuscarinic AgonistsNeuronsPhosphopyruvate HydratasePilocarpineRatsRats, Sprague-DawleyRats, TransgenicStatus EpilepticusTime FactorsConceptsAberrant mossy fiber sproutingMossy fiber sproutingStatus epilepticusNeuronal cell deathMesenchymal stem cellsMSC infusionIntravenous infusionRat modelCognitive functionMorris water maze testCognitive function preservationNumber of GAD67Water maze testVehicle-infused ratsMagnetic resonance imagingCell deathStem cellsSeizure frequencyFiber sproutingFunction preservationTimm stainingMaze testHippocampal neuronsImmunohistochemical stainingCognitive deterioration
2017
Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats.
Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Namioka T, Namioka A, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats. Journal Of Neurosurgery 2017, 127: 917-926. PMID: 28059661, DOI: 10.3171/2016.8.jns16240.Peer-Reviewed Original ResearchConceptsTransient middle cerebral artery occlusionRecombinant tissue plasminogen activatorMiddle cerebral artery occlusionRegional cerebral blood flowCerebral artery occlusionMesenchymal stem cellsHemorrhagic eventsArtery occlusionEndothelial dysfunctionRtPA therapyIntracerebral hemorrhageIntravenous infusionIncidence rateNormal salineIntravenous recombinant tissue plasminogen activatorRecombinant tissue plasminogen activator therapyTissue plasminogen activator therapyInfused mesenchymal stem cellsMatrix metalloproteinase-9 levelsEarly behavioral recoveryNon-rtPA groupAcute ischemic strokeVascular endothelial dysfunctionExperimental stroke modelsMetalloproteinase-9 levels
2016
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury
Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury. Neuroscience 2016, 335: 221-231. PMID: 27586052, DOI: 10.1016/j.neuroscience.2016.08.037.Peer-Reviewed Original ResearchConceptsSpinal cord injuryIntravenous infusionMesenchymal stem cellsCord injuryBone marrowBlood-spinal cord barrier integrityPhases of SCIChronic spinal cord injuryOpen-field locomotor functionContusive spinal cord injurySevere Contusive Spinal Cord InjuryVehicle-treated groupStem cellsAdult bone marrowBSCB leakageMSC infusionExtensive remyelinationMotor recoveryFunctional recoverySerotonergic fibersCorticospinal tractSystemic infusionFunctional improvementSpinal cordRat modelSynergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats
Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O. Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats. Physical Therapy 2016, 96: 1791-1798. PMID: 27174259, DOI: 10.2522/ptj.20150504.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDisease Models, AnimalInfusions, IntravenousMesenchymal Stem Cell TransplantationPhysical Conditioning, AnimalRatsRats, Sprague-DawleyStroke RehabilitationConceptsMiddle cerebral artery occlusionIntravenous infusionDaily rehabilitationMesenchymal stem cellsMSC infusionFunctional outcomePermanent middle cerebral artery occlusionLimb placement testCerebral artery occlusionRat stroke modelRat MCAO modelIntraluminal vascular occlusionMagnetic resonance imagingStem cellsAdult bone marrowArtery occlusionCerebral ischemiaMCAO modelVascular occlusionFunctional improvementLesion volumeTherapy groupTherapeutic effectStroke modelGroup 2
2015
[Treatment of cerebral ischemia using mesenchymal stem cell-clinical trial phase III].
Nakazaki M, Oka S, Sasaki M, Honmou O. [Treatment of cerebral ischemia using mesenchymal stem cell-clinical trial phase III]. Nippon Jibiinkoka Gakkai Kaiho 2015, 118: 93. PMID: 26495511, DOI: 10.3950/jibiinkoka.118.93.Peer-Reviewed Original Research
2011
Development of a Middle Cerebral Artery Occlusion Model in the Nonhuman Primate and a Safety Study of I.V. Infusion of Human Mesenchymal Stem Cells
Sasaki M, Honmou O, Radtke C, Kocsis JD. Development of a Middle Cerebral Artery Occlusion Model in the Nonhuman Primate and a Safety Study of I.V. Infusion of Human Mesenchymal Stem Cells. PLOS ONE 2011, 6: e26577. PMID: 22039510, PMCID: PMC3200343, DOI: 10.1371/journal.pone.0026577.Peer-Reviewed Original ResearchConceptsMiddle cerebral arteryMagnetic resonance imagingM1 occlusionCollateral circulationSevere groupVenous bloodOcclusion modelMesenchymal stem cellsMiddle cerebral artery occlusion modelM1 branchNHP stroke modelsExtensive collateral circulationArtery occlusion modelPost-operative courseMCA territory infarctionGreater functional improvementLeft sylvian fissureNonhuman primate modelFronto-temporal craniotomyExperimental stroke researchAfrican green monkeysStem cellsHMSC infusionTerritory infarctionNeurological examination