2023
Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imaging
2022
Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projections
2021
Postoperative spinal cord ischaemia: magnetic resonance imaging and clinical features
Yasuda N, Kuroda Y, Ito T, Sasaki M, Oka S, Ukai R, Nakanishi K, Mikami T, Shibata T, Harada R, Naraoka S, Kamada T, Kawaharada N. Postoperative spinal cord ischaemia: magnetic resonance imaging and clinical features. European Journal Of Cardio-Thoracic Surgery 2021, 60: 164-174. PMID: 33517384, DOI: 10.1093/ejcts/ezaa476.Peer-Reviewed Original ResearchMeSH KeywordsHumansInfarctionMagnetic Resonance ImagingSpinal CordSpinal Cord InjuriesSpinal Cord IschemiaConceptsIschemic spinal cord injurySpinal cord injuryMagnetic resonance imagingSpinal cord levelArtery territoryAortic surgeryAortic eventsCord levelCardiovascular surgeryAnterior spinal artery territoryPosterior spinal artery territoryResonance imagingThoracic spinal cord levelLumbar spinal cord levelsSpinal cord infarctionT2-weighted MRI scansSite of infarctionT2-weighted magnetic resonance imagesHigh-intensity areaCord infarctionInfarction patternArterial territoriesSerious complicationsClinical featuresCord injuryIntravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model
Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model. Brain Research 2021, 1757: 147296. PMID: 33516815, DOI: 10.1016/j.brainres.2021.147296.Peer-Reviewed Original ResearchConceptsBlood-spinal cord barrierQuantitative reverse transcription polymerase chain reactionIntravenous infusionDisease progressionMotor neuronsMSC groupLocomotor functionOpen-field locomotor functionPreservation of microvasculatureHind limb functionCommon clinical featuresEvans blue leakageMotor neuron lossReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionDevastating neurodegenerative diseaseBBB scoringBSCB functionRotarod testingClinical featuresNeuron lossLimb functionNeurotrophic factorCurative strategiesSpinal cord
2016
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury
Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury. Neuroscience 2016, 335: 221-231. PMID: 27586052, DOI: 10.1016/j.neuroscience.2016.08.037.Peer-Reviewed Original ResearchConceptsSpinal cord injuryIntravenous infusionMesenchymal stem cellsCord injuryBone marrowBlood-spinal cord barrier integrityPhases of SCIChronic spinal cord injuryOpen-field locomotor functionContusive spinal cord injurySevere Contusive Spinal Cord InjuryVehicle-treated groupStem cellsAdult bone marrowBSCB leakageMSC infusionExtensive remyelinationMotor recoveryFunctional recoverySerotonergic fibersCorticospinal tractSystemic infusionFunctional improvementSpinal cordRat model
2010
Focal experimental autoimmune encephalomyelitis in the lewis rat induced by immunization with myelin oligodendrocyte glycoprotein and intraspinal injection of vascular endothelial growth factor
Sasaki M, Lankford KL, Brown RJ, Ruddle NH, Kocsis JD. Focal experimental autoimmune encephalomyelitis in the lewis rat induced by immunization with myelin oligodendrocyte glycoprotein and intraspinal injection of vascular endothelial growth factor. Glia 2010, 58: 1523-1531. PMID: 20645414, DOI: 10.1002/glia.21026.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAntibodiesBlood-Brain BarrierCD3 ComplexDisease Models, AnimalEncephalomyelitis, Autoimmune, ExperimentalEnzyme-Linked Immunosorbent AssayFemaleFreund's AdjuvantInjections, SpinalLipidsMicroscopy, Electron, TransmissionMyelin ProteinsMyelin-Associated GlycoproteinMyelin-Oligodendrocyte GlycoproteinRatsRats, Inbred LewSpinal CordTime FactorsVascular Endothelial Growth Factor AConceptsMyelin oligodendrocyte glycoproteinVascular endothelial growth factorExperimental autoimmune encephalomyelitisIncomplete Freund's adjuvantBlood-brain barrierInflammatory demyelinating lesionsLewis ratsEndothelial growth factorDemyelinating lesionsEAE modelAutoimmune encephalomyelitisFreund's adjuvantIntraspinal injectionOligodendrocyte glycoproteinRecombinant rat myelin oligodendrocyte glycoproteinCentral nervous system locationsGrowth factorSensitized T cellsFocal experimental autoimmune encephalomyelitisRat myelin oligodendrocyte glycoproteinSite of injectionMyelin-forming cellsMOG immunizationExtensive demyelinationLymphocyte infiltration
2009
Convergence of Cells from the Progenitor Fraction of Adult Olfactory Bulb Tissue to Remyelinating Glia in Demyelinating Spinal Cord Lesions
Markakis EA, Sasaki M, Lankford KL, Kocsis JD. Convergence of Cells from the Progenitor Fraction of Adult Olfactory Bulb Tissue to Remyelinating Glia in Demyelinating Spinal Cord Lesions. PLOS ONE 2009, 4: e7260. PMID: 19787061, PMCID: PMC2747269, DOI: 10.1371/journal.pone.0007260.Peer-Reviewed Original Research
2008
Olfactory ensheathing cells exhibit unique migratory, phagocytic, and myelinating properties in the X‐irradiated spinal cord not shared by Schwann cells
Lankford KL, Sasaki M, Radtke C, Kocsis JD. Olfactory ensheathing cells exhibit unique migratory, phagocytic, and myelinating properties in the X‐irradiated spinal cord not shared by Schwann cells. Glia 2008, 56: 1664-1678. PMID: 18551623, DOI: 10.1002/glia.20718.Peer-Reviewed Original ResearchConceptsCentral nervous systemSpinal cordSchwann cellsOligodendrocyte progenitor cellsNormal central nervous systemMigratory propertiesOX-42 stainingNormal spinal cordOlfactory Ensheathing CellsMyelin-forming cellsDemyelinated lesionsHost axonsPhagocytic phenotypePoor survivalSubsequent lesionsNervous systemWhite matterCordLimited survivalOECsMigratory capabilityProgenitor cellsTransplantationX-irradiationLesions
2006
Molecular Reconstruction of Nodes of Ranvier after Remyelination by Transplanted Olfactory Ensheathing Cells in the Demyelinated Spinal Cord
Sasaki M, Black JA, Lankford KL, Tokuno HA, Waxman SG, Kocsis JD. Molecular Reconstruction of Nodes of Ranvier after Remyelination by Transplanted Olfactory Ensheathing Cells in the Demyelinated Spinal Cord. Journal Of Neuroscience 2006, 26: 1803-1812. PMID: 16467529, PMCID: PMC2605396, DOI: 10.1523/jneurosci.3611-05.2006.Peer-Reviewed Original ResearchConceptsDemyelinated spinal cordSpecific membrane domainsSpinal cordVoltage-gated ion channelsGreen fluorescent proteinTransplanted Olfactory Ensheathing CellsMature NodalMembrane domainsSpinal cord demyelinationOlfactory Ensheathing CellsVivo electrophysiological recordingsFluorescent proteinJuxtaparanodal domainsMolecular reconstructionIon channelsMature nodesRemyelinated axonsExtensive remyelinationUninjured axonsKv channelsDemyelinated axonsDonor ratsCentral axonsGlial cellsJuxtaparanodal region
2004
Autologous transplantation of expanded neural precursor cells into the demyelinated monkey spinal cord
Oka S, Honmou O, Akiyama Y, Sasaki M, Houkin K, Hashi K, Kocsis JD. Autologous transplantation of expanded neural precursor cells into the demyelinated monkey spinal cord. Brain Research 2004, 1030: 94-102. PMID: 15567341, DOI: 10.1016/j.brainres.2004.09.062.Peer-Reviewed Original ResearchConceptsNeural precursor cellsAutologous transplantationSpinal cordPrecursor cellsSubventricular zoneAdult primate brainMonkey spinal cordCentral nervous system axonsPresence of mitogensAdult nonhuman primatesNon-human primatesNeurological deficitsDemyelinated lesionsDorsal columnsIntraspinal injectionElectron microscopic examinationControl lesionsLesion sitePrimate brainPeripheral patternMyelinated axonsTransplantationLesionsNonhuman primatesCordIdentified Olfactory Ensheathing Cells Transplanted into the Transected Dorsal Funiculus Bridge the Lesion and Form Myelin
Sasaki M, Lankford KL, Zemedkun M, Kocsis JD. Identified Olfactory Ensheathing Cells Transplanted into the Transected Dorsal Funiculus Bridge the Lesion and Form Myelin. Journal Of Neuroscience 2004, 24: 8485-8493. PMID: 15456822, PMCID: PMC2605369, DOI: 10.1523/jneurosci.1998-04.2004.Peer-Reviewed Original ResearchConceptsSprague-DawleyOlfactory bulbTransection siteMyelinated axonsLesion zoneForm myelinOpen field locomotor behaviorAdult olfactory bulbTransection lesionTransplantation groupPattern of repairSD ratsSpinal cordLesion areaLesionsCellular elementsImproved locomotionAxonsLocomotor behaviorMyelinGFP cellsGreen fluorescent proteinCellsAnti-GFP antibodyDiscrete bundles
2001
Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons
Sasaki M, Honmou O, Akiyama Y, Uede T, Hashi K, Kocsis J. Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons. Glia 2001, 35: 26-34. PMID: 11424189, PMCID: PMC2605363, DOI: 10.1002/glia.1067.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBeta-GalactosidaseBone Marrow TransplantationCells, CulturedEthidiumGlial Fibrillary Acidic ProteinImmunohistochemistryMiceMice, TransgenicMyeloid Progenitor CellsNerve Fibers, MyelinatedNerve RegenerationNeurogliaRatsRats, WistarRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsBone marrow cellsSpinal cordMyelin-forming cellsMarrow cellsDemyelinated rat spinal cordRat spinal cord axonsDorsal column lesionBone marrow cell fractionRat spinal cordX-irradiation treatmentSpinal cord axonsLacZ transgenic miceSchwann cell myelinationCell fractionCell transplantation techniquesDorsal funiculusPeripheral patternTransgenic miceTransplantation techniquesHematopoietic stem cellsIsolated cell fractionsCordFemoral bonePrecursor cellsTransplantation
2000
[Remyelination by Schwann cell transplantation for CNS demyelinated axons: functional comparison with developmental myelination].
Imaizumi T, Tsushima S, Sasaki M, Akiyama Y, Hashi K. [Remyelination by Schwann cell transplantation for CNS demyelinated axons: functional comparison with developmental myelination]. Brain And Nerve 脳と神経 2000, 52: 701-7. PMID: 11002480.Peer-Reviewed Original ResearchConceptsCompound action potentialConduction velocitySC transplantationAdult ratsDemyelinated axonsHigh-frequency stimulationDay old pupsLower conduction velocityNormal adult ratsSC myelinationSpinal cordFrequency stimulationRat dorsalHistological examinationStimulus trainsTransplantationOld pupsRemyelinationAmplitude decrementAction potentialsNormal adultsAxonsRatsNormal DCsMyelination[Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons].
Imaizumi T, Lankford K, Kocsis J, Sasaki M, Akiyama Y, Hashi K. [Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons]. Brain And Nerve 脳と神経 2000, 52: 609-15. PMID: 10934721.Peer-Reviewed Original ResearchConceptsDemyelinated CNS axonsSchwann cellsBrain cellsSpinal cordConduction velocityCNS axonsAdult rat spinal cordFronto-temporal lobesRat spinal cordAdult Schwann cellsDemyelination of axonsMyelin-forming cellsBlock of conductionAdult olfactoryDemyelinated diseasesDemyelinated axonsLess myelinationHistological examinationCNS tissueTherapeutic approachesStimulus trainsDay 28Transplanted OECsAmplitude decrementRemyelination