1997
Mutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts
Nguyen H, Estacion M, Gargus J. Mutations Causing Achondroplasia and Thanatophoric Dysplasia Alter bFGF-Induced Calcium Signals in Human Diploid Fibroblasts. Human Molecular Genetics 1997, 6: 681-688. PMID: 9158142, DOI: 10.1093/hmg/6.5.681.Peer-Reviewed Original ResearchMeSH KeywordsAchondroplasiaBradykininCalciumCell LineDiploidyFibroblast Growth Factor 2FibroblastsHomozygoteHumansMutationProtein-Tyrosine KinasesReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptor, Fibroblast Growth Factor, Type 2Receptor, Fibroblast Growth Factor, Type 3Receptors, Fibroblast Growth FactorRNA SplicingSignal TransductionThanatophoric DysplasiaConceptsThanatophoric dysplasiaCalcium signalsBasic fibroblast growth factorCell linesCalcium-imaging analysesFibroblast growth factorFibroblast growth factor receptor (FGFR) gene familyHuman diploid fibroblastsPathophysiological mechanismsIntracellular calciumBlinded studyFGFR3 mutationsUnresponsive linesReceptor functionHereditary disorderTransient increaseGrowth factorDiploid fibroblastsControl cellsBone developmentFibroblast cell lineCalcium wavesReceptor gene familyHeterogeneous signalsAchondroplasia
1991
Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts.
Estacion M. Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts. The Journal Of Physiology 1991, 436: 603-620. PMID: 1648132, PMCID: PMC1181524, DOI: 10.1113/jphysiol.1991.sp018569.Peer-Reviewed Original ResearchConceptsFura-2 fluorescencePipette solutionIon substitution experimentsGTP gamma SReversal potentialBradykinin stimulationPresence of GTPHuman dermal fibroblastsGamma SPatch-clamp techniqueNon-specific cation channelsBradykinin additionDermal fibroblastsIntracellular calciumLittle voltage dependencePatch-clamped cellsTail current analysisAcute responseInward currentsCalcium ionophoreOutward currentsNear baselineCation channelsElectrophysiological responsesBradykinin