2024
Cotranslational molecular condensation of cochaperones and assembly factors facilitates axonemal dynein biogenesis
Li Y, Xu W, Cheng Y, Djenoune L, Zhuang C, Cox A, Britto C, Yuan S, Wang S, Sun Z. Cotranslational molecular condensation of cochaperones and assembly factors facilitates axonemal dynein biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2402818121. PMID: 39541357, PMCID: PMC11588059, DOI: 10.1073/pnas.2402818121.Peer-Reviewed Original ResearchConceptsDynein axonemal assembly factorsAssembly factorsCytosolic fociOuter dynein armsMacromolecular machinesAxonemal dyneinsAssembly hubDynein armsMolecular condensateLiquid-liquid phase separationCochaperoneEncoding mRNAFoci formationCiliary motilityStable interactionLRRC6Functional significanceRUVBL1DyneinMRNAAssembly of multiple componentsAssemblyPotential mechanismsRUVBL2Biogenesis
2009
Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.
Yuan S, Sun Z. Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos. Journal Of Visualized Experiments 2009 PMID: 19488022, PMCID: PMC2762915, DOI: 10.3791/1113.Peer-Reviewed Original ResearchConceptsZebrafish embryosOverexpression of EGFPMorpholino oligonucleotide injectionKnockdown of PKD2Microinjection of RNAGerm-line chimerasMicroinjection of mRNACell fate mappingMorpholino microinjectionAutosomal dominant polycystic kidney diseaseGene functionMisexpression studiesMorpholino antisenseMorpholino oligosGene knockdownTransgenic animalsGFP expressionOligonucleotide injectionEGFP mRNAPhenotype analysisEmbryosGene screeningMRNABroad applicationsPKD2