2010
Oral N-acetylcysteine rescues lethality of hepatocyte-specific Gclc-knockout mice, providing a model for hepatic cirrhosis
Chen Y, Johansson E, Yang Y, Miller ML, Shen D, Orlicky DJ, Shertzer HG, Vasiliou V, Nebert DW, Dalton TP. Oral N-acetylcysteine rescues lethality of hepatocyte-specific Gclc-knockout mice, providing a model for hepatic cirrhosis. Journal Of Hepatology 2010, 53: 1085-1094. PMID: 20810184, PMCID: PMC2970663, DOI: 10.1016/j.jhep.2010.05.028.Peer-Reviewed Original ResearchAcetylcysteineAdministration, OralAnimalsAntioxidantsBase SequenceCytokinesDisease Models, AnimalDNA PrimersGene Expression ProfilingGlutamate-Cysteine LigaseGlutathioneHepatocytesLiverLiver CirrhosisMiceMice, KnockoutMicroscopy, Electron, TransmissionMitochondria, LiverOxidative StressRNA, Messenger
2008
Knock-In Mouse Lines Expressing either Mitochondrial or Microsomal CYP1A1: Differing Responses to Dietary Benzo[a]pyrene as Proof of Principle
Dong H, Dalton TP, Miller ML, Chen Y, Uno S, Shi Z, Shertzer HG, Bansal S, Avadhani NG, Nebert DW. Knock-In Mouse Lines Expressing either Mitochondrial or Microsomal CYP1A1: Differing Responses to Dietary Benzo[a]pyrene as Proof of Principle. Molecular Pharmacology 2008, 75: 555-567. PMID: 19047483, PMCID: PMC2684908, DOI: 10.1124/mol.108.051888.Peer-Reviewed Original ResearchConceptsMitochondrial importCryptic targeting signalMitochondrial-targeting signalSignal recognition particleInner mitochondrial membraneDifferent substrate specificitiesMouse linesCYP1A1 proteinTargeting signalsRecognition particleCYP1A1 enzymeSubstrate specificityMitochondrial membraneEndoplasmic reticulumBaP toxicityPhysiological functionsTerminal processingProteinCytosolic peptidasesProof of principleMutationsEnzymeInducer propertiesCYP1B1 mRNAMicrosomal CYP1A1